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Effects of β-carotene on cell viability and antioxidant status of hepatocytes from chronically ethanol-fed rats

Published online by Cambridge University Press:  09 March 2007

Suh-Ching Yang ,
Chi-Chang Huang ,
Jan-Show Chu  and
Jiun-Rong Chen
Suh-Ching Yang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsin Street, Taipei 110, Taiwan, Republic of China
Chi-Chang Huang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsin Street, Taipei 110, Taiwan, Republic of China
Jan-Show Chu
Affiliation:
Department of Pathology, Taipei Medical University, Taipei, Taiwan, Republic of China
Jiun-Rong Chen*
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsin Street, Taipei 110, Taiwan, Republic of China
*
*Corresponding author: fax +886 2 2737 3112, email [email protected]
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Abstract

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The purpose of the present study was to evaluate the effects of β-carotene on the cell viability and antioxidant status of hepatocytes from chronically ethanol-fed rats. Rats in the ethanol group were given an ethanol-containing liquid diet that provided 36% of total energy as ethanol, while rats in the control group were fed an isoenergetic diet without ethanol. After 4 weeks, hepatocytes were taken out and cultured for 24 h. Hepatocytes from the rats in the control and ethanol groups were cultured in medium without (HC, HE) or with β-carotene (HC+B, HE+B). The results showed that lactate dehydrogenase leakage was significantly increased in the HE compared with that in the HC group. However, lactate dehydrogenase leakage of the HE+B group was similar to that of the HC group. When compared with the HC group, activities of glutathione peroxidase and catalase in the HE group were significantly decreased by 54 and 31%, respectively. Catalase activity in the HE+B group was significantly increased by 61% compared with that in the HE group. However, activities of glutathione reductase and superoxide dismutase showed no difference among the groups. The level of glutathione in the HC+B and HE+B groups was significantly increased to 155 and 143% compared with those in the HC and HE groups, respectively. The concentration of lipid peroxides showed no difference among the groups. The present results demonstrate that β-carotene improved the cell viability of hepatocytes, and increased catalase activities and glutathione levels in hepatocytes from chronically ethanol-fed rats.

Keywords

β-CaroteneChronic ethanol feedingAntioxidant statusHepatocytes
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 2 , August 2004 , pp. 209 - 215
Copyright
Copyright © The Nutrition Society 2004

References

Ahmed, S, Leo, MA & Lieber, CSInteraction between alcohol and beta-carotene in patients with alcoholic liver disease. Am J Clin Nutr (1994) 60, 430–436.CrossRefGoogle ScholarPubMed
Alam, SQ & Alam, BSLipid peroxide, α-tocopherol and retinoid levels in plasma and liver of rats fed diets containing β-carotene and 13-cis-retinoic acids. J Nutr (1983) 113, 2608–2614.CrossRefGoogle Scholar
Anderson, MEEnzymatic and chemical methods for the determination of glutathione. In Glutathione: Chemical, Biochemical and Medical Aspec, vol. A. pp. 339–365 [Dolphin, D, Poulson, R & Avramovic, O editors]. New York: John Wiley & Sons. (1989)Google Scholar
Bailey, SM & Cunningham, CCEffect of dietary fat on chronic ethanol-induced oxidative stress in hepatocytes. Alcohol Clin Exp Res (1999) 23, 1210–1218.CrossRefGoogle ScholarPubMed
Berry, MN & Friend, DSHigh-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol (1969) 43, 506–520.CrossRefGoogle Scholar
Bertram, JS, Pung, A, Churley, M, Kappock, TJ, Wilkins, LR & Conney, RVDiverse carotenoids protect against chemically induced neoplastic transformation. Carcinogenesis (1991) 12, 671–678.CrossRefGoogle ScholarPubMed
Comport, M, Benedetti, A & Cheli, FStudies on in vitro peroxidation of liver lipids in ethanol-treated rats. Lipids (1973) 8, 498–502.CrossRefGoogle Scholar
Cunningham, CC & Spach, PIThe effect of chronic ethanol consumption on the lipids in liver mitochondria. Ann N Y Acad Sci (1987) 492, 181–192.CrossRefGoogle ScholarPubMed
Department of Health Taiwan Analysis of the Main Causes of Death in Taiwan for the Year 2002 Taiwan: Department of Health (2003)Google Scholar
Diplock, ATAntioxidant nutrients and disease prevention: an overview. Am J Clin Nutr (1991) 53, 189S–193S.Google ScholarPubMed
Dufour, DR, Lott, JA, Nolte, FS, Gretch, DR, Koff, RS & Seeff, LBDiagnosis and monitoring of hepatic injury. I Performance characteristics of laboratory tests. Clin Chem (2000) 46, 2027–2049.CrossRefGoogle ScholarPubMed
Ek, B, Halberg, C, Sjogren, KG, & Hjalmarson, AReoxygenation-induced cell damage of isolated neonatal rat ventricular myocytes can be reduced by chain-breaking antioxidants. Free Radic Biol Med (1994) 16, 117–121.CrossRefGoogle ScholarPubMed
Esterbauer, H & Cheeseman, KHDetermination of aldehydic lipid peroxidation products: malonaldehyde and 4–hydroxynonenal. Methods Enzymol (1990) 186, 407–421.CrossRefGoogle ScholarPubMed
Ishii, H, Kurose, I & Kato, S, Pathogenesis of alcoholic liver disease with particular emphasis on oxidative stress. J Gastroenterol Hepatol (1997) 12, 272–282.CrossRefGoogle ScholarPubMed
Kawase, T, Kato, S & Lieber, CS, Lipid peroxidation and antioxidant defense system in rat liver after chronic ethanol feeding. Hepatology (1989) 10, 815–821.CrossRefGoogle ScholarPubMed
Kim-Jun, HInhibitory effects of a and β–carotene in croton oil–induced or enzymatic lipid peroxidation and hydroperoxide production in mouse skin epidermis. Int J Biochem (1993) 25, 911–915.CrossRefGoogle ScholarPubMed
Krinsky, NIAntioxidant functions of carotenoids. Free Radic Biol Med (1989) 7, 617–635.CrossRefGoogle ScholarPubMed
Krinsky, NI & Deneke, SMInteraction of oxygen and oxyradicals with carotenoids. J Natl Cancer Inst (1982) 69, 205–210.Google ScholarPubMed
Kunert, KJ & Tappel, ALThe effect of vitamin C and bcarotene on in vitro lipid peroxidation in guinea pig as measured by pentane and ethane production. Lipids (1983) 11, 271–274.CrossRefGoogle Scholar
Lawlor, SM & O'Brien, NMModulation of paraquat toxicity by β–carotene at low oxygen partial pressure in chicken embryo fibroblasts. Br J Nutr (1997) 77, 133–140.CrossRefGoogle ScholarPubMed
Leo, MA, Rosman, AS & Lieber, CS, Differential depletion of carotenoids and tocopherol in liver disease. Hepatology (1993) 17, 977–986.Google ScholarPubMed
Lieber, CSHepatic metabolic and toxic effect of ethanol: 1991 update. Alcohol Clin Exp Res (1991) 15, 573–592.CrossRefGoogle ScholarPubMed
Lieber, CSRoles of oxidative stress and antioxidant therapy in alcoholic and nonalcoholic liver diseases. Adv Pharmacol (1997) 38, 601–628.CrossRefGoogle ScholarPubMed
Lieber, CS & DeCarli, LMAnimal models of chronic ethanol toxicity. Methods Enzymol (1994) 233, 585–594.CrossRefGoogle ScholarPubMed
Lomnitski, L, Bar-Natan, R, Sklan, D, & Grossman, SThe interaction between β–carotene and lipoxygenase in plant and animal systems. Biochim Biophys Acta (1993) 1167, 331–338.CrossRefGoogle ScholarPubMed
Lowry, OH, Rosebrough, NJ, Farr, A, & Randall, RJProtein measurement with the Folin phenol reagent. J Biol Chem (1951) 193, 265–275.CrossRefGoogle ScholarPubMed
Lück, HCatalase. In Methods of Enzymatic Analysis, pp. 885–888. [Bergmeyer, HU, editor]. New York: Academic Press (1963)Google Scholar
Martin, KR, Faila, ML & Smith, JCBeta-carotene and lutein protect HepG2 human liver cells against oxidant-induced damage. J Nutr (1996) 126, 2098–2106.CrossRefGoogle ScholarPubMed
Moldeus, P, Hogberg, J & Orrenius, SIsolation and use of liver cells. Methods Enzymol (1978) 52, 60–71.CrossRefGoogle ScholarPubMed
Morton, S & Mitchell, MCEffects of chronic ethanol feeding on glutathione turnover in the ra. Biochem Pharmacol (1985) 34, 1559–1563.CrossRefGoogle Scholar
Müller, A, Sies, HRole of alcohol dehydrogenase activity and the acetaldehyde in ethanol-induced ethane and pentane production by isolated perfused rat liver. Biochem J (1982) 206, 153–156.CrossRefGoogle ScholarPubMed
Navder, KP, Baraona, E & Lieber, CSPolyenylphosphatidylcholine attenuates alcohol-induced fatty liver and hyperlipidemia in rats. Nutrition (1997) 127, 1800–1806.Google ScholarPubMed
Nebot, C, Moutet, M, Huet, P, Xu, JZ, Yadan, JC & Chaudiere, JSpectrophotometric assay of superoxide dismutase activity based on the activated autoxidation of a tetracyclic catechol. Anal Biochem (1993) 214, 442–451.CrossRefGoogle ScholarPubMed
Nordmann, R, Ribiere, C & Rouach, HImplication of free radical mechanisms in ethanol-induced cellular injury. Free Radic Biol Med (1992) 12, 219–240.CrossRefGoogle ScholarPubMed
Oh, SI, Kim, CI, Chun, HJ & Park, SCChronic ethanol consumption affects glutathione status in rat liver. J Nutr (1998) 128, 758–763.CrossRefGoogle ScholarPubMed
Paglia, DE & Valentine, WNStudies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med (1967) 70, 158–169.Google ScholarPubMed
Palozza, P & Krinsky, NIThe inhibition of radical-initiated peroxidation of microsomal lipids by both a-tocopherol and bcarotene. Free Radic Biol Med (1991) 11, 407–414.CrossRefGoogle Scholar
Palozza, P & Krinsky, NIAntioxidant effects of carotenoids in vivo and in vitro: an overview. Methods Enzymol (1992) 213, 403–420.CrossRefGoogle ScholarPubMed
Pirola, RC & Lieber, CSEnergy wastage in rats given drugs that induce microsomal enzyme. J Nutr (1975) 105, 1544–1548.CrossRefGoogle Scholar
Polavarapu, R, Spitz, DR, Sim, JEFollansbee, MH, Oberley, LW, Rahemtulla, A & Nanji, AAIncreased lipid peroxidation and impaired antioxidant enzyme function is associated with pathological liver injury in experimental alcoholic liver disease in rats fed diets high in corn oil and fish oil. Hepatology (1998) 27, 1317–1323.CrossRefGoogle ScholarPubMed
Reinke, LA, Lai, EK, DuBose, CM& MacCay, PBReactive free radical generation in vivo in heart and liver of ethanol-fed rats: correlation with radical formation in vitro. Proc Natl Acad Sci U S A (1987) 84, 9223–9227.CrossRefGoogle ScholarPubMed
Rouach, H, Fataccioli, V, Gentil, MFrench, SW, Morimoto, M & Nordmann, REffect of chronic ethanol feeding on lipid peroxidation and protein oxidation in relation to liver pathology. Hepatology (1997) 25, 351–355.CrossRefGoogle ScholarPubMed
Schisler, NJ & Singh, SMEffect of ethanol in vivo on enzymes which detoxify oxygen free radicals. Free Radic Biol Med (1989) 7, 117–123.CrossRefGoogle ScholarPubMed
Shaw, S, Jayatilleke, E, Ross, WAGordon, ER & Lieber, CSEthanol-induced lipid peroxidation: potentiation by long-term alcohol feeding and attenuation by methionine. J Lab Clin Med (1981) 98, 417–422.Google ScholarPubMed
Wei, RR, Wamer, WG, Lambert, LA& Kornhauser, Aβ–Carotene uptake and effects on intracellular levels of retinal in vitro. Nutr Cancer (1998) 30, 53–58.CrossRefGoogle Scholar
Williams, AJ & Barry, REFree radical generation by neutrophils: a potential mechanism of cellular injury in acute alcoholic hepatitis. Gut (1987) 28, 1157–1161.CrossRefGoogle ScholarPubMed