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Effects of calcium ionophore on vitamin E-deficient rat muscle

Published online by Cambridge University Press:  09 March 2007

Joanne Phoenix ,
Richard H. T. Edwards ,
Malcolm J. Jackson  and
Malcolm J. Jackson
Joanne Phoenix
Affiliation:
Muscle Research Centre, Department of Medicine, University of Liverpool, Liverpool L69 3BX
Richard H. T. Edwards
Affiliation:
Muscle Research Centre, Department of Medicine, University of Liverpool, Liverpool L69 3BX
Malcolm J. Jackson
Affiliation:
Muscle Research Centre, Department of Medicine, University of Liverpool, Liverpool L69 3BX
Malcolm J. Jackson
Affiliation:
Muscle Research Centre, Department of Medicine, University of Liverpool, Liverpool L69 3BX
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Abstract

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Damage to skeletal muscles may be mediated via free radicals or intracellular calcium overload. To look for inter-relationships between these pathways we have examined the effect of intracellular Ca overload on muscles from rats fed on either a vitamin E-deficient or vitamin E-sufficient diet and assessed the non-enzymic lipid peroxidation in these muscles by examining the production of thiobarbituric acid reactive substances by homogenates. Vitamin E-deficient muscles were more susceptible to Ca-induced intracellular enzyme efflux and this was acutely corrected by supplementation of the external medium with 230 μmol α-tocopherol/l. Vitamin E-deficient muscles showed increased levels of basal lipid peroxides and were more susceptible to iron-catalysed lipid peroxidation. Addition of the Ca ionophore A23187 increased lipid peroxidation in vitamin E-deficient muscle homogenates, but had the opposite effect in vitamin E-sufficient muscles. These results demonstrate that vitamin E-deficient muscle has an increased susceptibility to intracellular Ca overload, but that this effect cannot be explained by a direct stimulatory effect of the ionophore on non-enzymic lipid peroxidation.

Keywords

Calcium ionophoreVitamin E deficiencyMuscleRat
Type
Micronutrients
Information
British Journal of Nutrition , Volume 64 , Issue 1 , July 1990 , pp. 245 - 256
Copyright
Copyright © The Nutrition Society 1990

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