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Effects of age, and protein malnutrition followed by a balanced diet on the non-parallel change in digestive enzymes in the pancreas and their secretion in the rat

Published online by Cambridge University Press:  09 March 2007

Josiane Prost ,
Jacques Belleville  and
Carole Valantin-Rollet
Josiane Prost
Affiliation:
Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 – 21004 Dijon Cedex, France
Jacques Belleville
Affiliation:
Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 – 21004 Dijon Cedex, France
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Abstract

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1. Ninety male Wistar rats were divided into two groups. A control group (C) was fed on a balanced diet, containing 200 g protein/kg for 51 d. An experimental group (E) was fed on a low-protein diet containing 50 g protein/kg for 28 d (PM), and then on a balanced diet for 23 d (BR). At different days of PM and BR, the pancreas and the pancreatic juice were collected 40 min after injection of 0.1 mCi [3H]leucine. The amounts of amylase (EC 3.2.1.1), trypsinogen 2 (EC 3.4.21.4), chymotrypsinogen 1 (EC 3.4.21.1) and lipase (EC 3.1.1.3) were determined after separation by the isoelectric focussing technique. Incorporation of [3H]leucine into the four hydrolases of pancreatic juice and pancreas was also determined.

2. In control rats a progressive increase in the concentration of digestive enzymes and the amounts secreted were observed with age. Maturation was reached when the rats were 9 weeks old. In rats E, PM inhibited maturation of the pancreas. However, individual enzymes were not affected to the same extent and at the same time. As soon as re-feeding was initiated, pancreas maturation took place and a significant increase in these variables was observed. The increases varied according to the hydrolase and did not appear at the same time.

3. In control rats, a preferential secretion of newly synthesized enzymes was observed in young rats, whereas with age, the proportion of newly synthesized enzymes excreted decreased slowly. In group E rats, at the beginning of PM, the proportion of newly synthesized enzymes secreted was very low and increased with time.

4. In rats C and E, our results indicated a non-parallelism between pancreatic enzyme levels and amounts secreted. This non-parallelism was different in both groups, it was changed with age and pancreas maturation in group C, and according to nutritional state in group E.

Type
General Nutrition papers
Information
British Journal of Nutrition , Volume 60 , Issue 3 , November 1988 , pp. 619 - 631
Copyright
Copyright © The Nutrition Society 1988

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