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The effect of triacylglycerol-fatty acid positional distribution on postprandial metabolism in subcutaneous adipose tissue

Published online by Cambridge University Press:  09 March 2007

Lucinda K. M. Summers ,
Barbara A. Fielding ,
Vera Ilic ,
Paul T. Quinlan  and
Keith N. Frayn
Lucinda K. M. Summers
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Barbara A. Fielding
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Vera Ilic
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Paul T. Quinlan
Affiliation:
Unilever Research Colworth Laboratory, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK
Keith N. Frayn*
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
*
*Corresponding author:Dr K. N. Frayn, fax +44 (0)1865 224652, email [email protected]
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Abstract

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We hypothesized that fatty acids at the sn−2 position of chylomicron triacylglycerol are preferentially released into the venous plasma (rather than being taken up and stored in the adipocytes) after hydrolysis by lipoprotein lipase (EC 3.1.1.34) in adipose tissue. Arterio–venous differences across adipose tissue were studied in eight healthy subjects on two occasions for 6 h after ingestion of different structured triacylglycerols rich in palmitic acid either at the sn−2 or the sn−1,3 positions. In particular the specific fatty acids making up lipoprotein fractions and plasma non-esterified fatty acids were analysed. After the different meals there were no differences between either postprandial arterialized or venous plasma metabolite concentrations. Chylomicron triacylglycerol extraction in adipose tissue was the same following the two types of fat. There was no difference between the specific fatty acid composition of the postprandial non-esterified fatty acid release from adipose tissue after ingestion of the two triacylglycerols, indicating that there was no preferential release of a saturated fatty acid at the sn−2 position.

Keywords

TriacylglycerolsAdipose tissueLipoprotein lipasePostprandial metabolism
Type
Human and Clinical Nutrition
Information
British Journal of Nutrition , Volume 79 , Issue 2 , February 1998 , pp. 141 - 147
Copyright
Copyright © The Nutrition Society 1998

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