Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-26T01:44:26.316Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of triacylglycerol-fatty acid positional distribution on postprandial metabolism in subcutaneous adipose tissue

Published online by Cambridge University Press:  09 March 2007

Lucinda K. M. Summers ,
Barbara A. Fielding ,
Vera Ilic ,
Paul T. Quinlan  and
Keith N. Frayn
Lucinda K. M. Summers
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Barbara A. Fielding
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Vera Ilic
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
Paul T. Quinlan
Affiliation:
Unilever Research Colworth Laboratory, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK
Keith N. Frayn*
Affiliation:
Oxford Lipid Metabolism Group, Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford OX2 6HE, UK
*
*Corresponding author:Dr K. N. Frayn, fax +44 (0)1865 224652, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

We hypothesized that fatty acids at the sn−2 position of chylomicron triacylglycerol are preferentially released into the venous plasma (rather than being taken up and stored in the adipocytes) after hydrolysis by lipoprotein lipase (EC 3.1.1.34) in adipose tissue. Arterio–venous differences across adipose tissue were studied in eight healthy subjects on two occasions for 6 h after ingestion of different structured triacylglycerols rich in palmitic acid either at the sn−2 or the sn−1,3 positions. In particular the specific fatty acids making up lipoprotein fractions and plasma non-esterified fatty acids were analysed. After the different meals there were no differences between either postprandial arterialized or venous plasma metabolite concentrations. Chylomicron triacylglycerol extraction in adipose tissue was the same following the two types of fat. There was no difference between the specific fatty acid composition of the postprandial non-esterified fatty acid release from adipose tissue after ingestion of the two triacylglycerols, indicating that there was no preferential release of a saturated fatty acid at the sn−2 position.

Keywords

TriacylglycerolsAdipose tissueLipoprotein lipasePostprandial metabolism
Type
Human and Clinical Nutrition
Information
British Journal of Nutrition , Volume 79 , Issue 2 , February 1998 , pp. 141 - 147
Copyright
Copyright © The Nutrition Society 1998

References

Åkesson, B, Gronowitz, S, Herslof, B & Ohlson, R (1978) Absorption of synthetic, stereochemically defined acylglycerols in the rat. Lipids 13, 338–343.CrossRefGoogle ScholarPubMed
Bergman, EN, Havel, RJ, Wolfe, BM & Bohmer, T (1971) Quantitative studies of the metabolism of chylomicron triglycerides and cholesterol by liver and extrahepatic tissues of sheep and dogs. Journal of Clinical Investigation 50, 1831–1839.CrossRefGoogle Scholar
Braun, J & Severson, D (1992) Regulation of the synthesis, processing and translocation of lipoprotein lipase. Biochemical Journal 287, 337–347.CrossRefGoogle ScholarPubMed
Carnielli, VP, Luijendijk, IHT, van Beek, RHT, Boerma, GJM, Degenhart, HJ & Sauer, PJJ (1995) Effect of dietary triacylgly-cerol fatty acid positional distribution on plasma lipid classes and their fatty acid composition in preterm infants. American Journal of Clinical Nutrition 62, 776–781.CrossRefGoogle ScholarPubMed
Coppack, SW, Frayn, KN, Humphreys, SM, Whyte, PL & Hockaday, TD (1990) Arteriovenous differences across human adipose tissue and forearm tissues after overnight fast. Metabolism 39, 384–390.CrossRefGoogle ScholarPubMed
de Fouw, N, Kivits, GAA, Quinlan, PT & van Nielen, WGL (1994) Absorption of isomeric, palmitic acid-containing triacylgly-cerols resembling human milk fat in the adult rat. Lipids 29, 765–770.CrossRefGoogle ScholarPubMed
Ebenbichler, CF, Kirchmair, R, Egger, C & Patsch, JR (1995) Postprandial state and atherosclerosis. Current Opinion in Lipidology 6, 286–290.CrossRefGoogle ScholarPubMed
Fielding, B, Humphreys, S, Shadid, S & Frayn, K (1995) Plasma mono-, di- and triacylglycerol measurements in a study of fat uptake by human adipose tissue in vivo. Biochemical Society Transactions 23, 487S.CrossRefGoogle Scholar
Fielding, BA, Callow, J, Owen, M, Samra, JS, Matthews, DR & Frayn, KN (1996) Postprandial lipemia: the origin of an early peak studied by specific dietary fatty acid intake during sequential meals. American Journal of Clinical Nutrition 63, 36–41.CrossRefGoogle ScholarPubMed
Filer, LJ, Mattson, FH & Fomon, SJ (1969) Triglyceride configuration and fat absorption by the human infant. Journal of Nutrition 99, 293–298.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Sloane-Stanley, G (1956) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle Scholar
Frayn, KN, Coppack, SW, Fielding, BA & Humphreys, SM (1995) Coordinated regulation of hormone-sensitive lipase and lipo-protein lipase in human adipose tissue in vivo: implications for the control of fat storage and fat mobilization. Advances in Enzyme Regulation 35, 163–178.CrossRefGoogle Scholar
Frayn, KN, Coppack, SW & Humphreys, SM (1993) Subcutaneous adipose tissue metabolism studied by local catheterization. International Journal of Obesity 17, S18–S21.Google ScholarPubMed
Frayn, KN, Coppack, SW, Humphreys, SM & Whyte, PL (1989) Metabolic characteristics of human adipose tissue in vivo. Clinical Science 76, 509–516.CrossRefGoogle ScholarPubMed
Frayn, KN, Shadid, S, Hamlani, R, Humphreys, SM, Clark, ML, Fielding, BA, Boland, O & Coppack, SW (1994) Regulation of fatty acid movement in human adipose tissue in the post-absorptive-to-postprandial transition. American Journal of Physiology 266, E308–E317.Google ScholarPubMed
Heimberg, M, Dunn, GD & Wilcox, HG (1974) The derivation of plasma-free fatty acids from dietary neutral fat in man. Journal of Laboratory and Clinical Medicine 83, 393–402.Google ScholarPubMed
Humphreys, SM, Fisher, RM & Frayn, KN (1990) Micro-method for measurement of sub-nanomole amounts of triacylglycerol. Annals of Clinical Biochemistry 27, 597–598.CrossRefGoogle ScholarPubMed
Larsen, OA, Lassen, NA & Quaade, F (1966) Blood flow through human adipose tissue determined with radioactive xenon. Acta Physiologica Scandinavica 66, 337–345.CrossRefGoogle ScholarPubMed
Mortimer, B-C, Holthouse, DJ, Martins, IJ, Stick, RV & Redgrave, TG (1994) Effects of triacylglycerol-saturated acyl chains on the clearance of chylomicron-like emulsions from the plasma of the rat. Biochimica et Biophysica Acta 1211, 171–180.CrossRefGoogle ScholarPubMed
Mortimer, B-C, Simmonds, WJ, Joll, CA, Stick, RV & Redgrave, TG (1988) Regulation of the metabolism of lipid emulsion model lipoproteins by a saturated acyl chain at the 2-position of triacylglycerol. Journal of Lipid Research 29, 713–720.CrossRefGoogle Scholar
Myher, JJ, Kuksis, A, Yang, LY & Marai, L (1987) Stereochemical course of intestinal absorption and transport of mustard-seed oil triacylglycerols in the rat. Biochemistry and Cell Biology 65, 811–821.CrossRefGoogle ScholarPubMed
Patsch, JR (1994) Triglyceride-rich lipoproteins and atherosclerosis. Atherosclerosis 110 Suppl., S23–S26.CrossRefGoogle ScholarPubMed
Potts, JL, Fisher, RM, Humphreys, SM, Coppack, SW, Gibbons, GF & Frayn, KN (1991) Peripheral triacylglycerol extraction in the fasting and post-prandial states. Clinical Science 81, 621–626.CrossRefGoogle ScholarPubMed
Pufal, DA, Quinlan, PT & Salter, AM (1995) Effect of dietary triacylglycerol structure on lipoprotein metabolism: A comparison of the effects of dioleoylpalmitoylglycerol in which palmitate is esterified to the 2- or 1(3)-position of the glycerol. Biochimica et Biophysica Acta 1258, 41–48.CrossRefGoogle ScholarPubMed
Redgrave, TG, Kodali, DR & Small, DM (1988) The effect of triacyl-sn−glycerol structure on the metabolism of chylomicrons and triacylglycerol-rich emulsions in the rat. Journal of Biological Chemistry 263, 5118–5123.CrossRefGoogle Scholar
Samra, JS, Frayn, KN, Giddings, JA, Clark, ML & Macdonald, IA (1995) Modification and validation of a commercially available portable detector for measurement of adipose tissue blood flow. Clinical Physiology 15, 241–248.CrossRefGoogle ScholarPubMed
Scow, RO (1977) Metabolism of chylomicrons in perfused adipose and mammary tissue of the rat. Federation Proceedings 36, 182–185.Google ScholarPubMed
Summers, LKM, Fielding, BA, Ilic, V & Frayn, KN (1997) The effect of body mass index on postprandial non-esterified fatty acid suppression. Proceedings of the Nutrition Society 56, 95A.Google Scholar
Summers, LKM, Samra, JS, Humphreys, SM, Morris, RJ & Frayn, KN (1996) Subcutaneous abdominal adipose tissue blood flow: variation within and between subjects and relationship to obesity. Clinical Science 91, 679–683.CrossRefGoogle ScholarPubMed
Tuten, T, Robinson, KA & Sgoutas, DS (1993) Discordant results for determinations of triglycerides in pig sera. Clinical Chemistry 39, 125–128.CrossRefGoogle ScholarPubMed
Yang, LY & Kuksis, A (1991) Apparent convergence (at 2-monoacylglycerol level) of phosphatidic acid and 2-monoacylglycerol pathways of synthesis of chylomicron triacylglycerols. Journal of Lipid Research 32, 1173–1186.CrossRefGoogle ScholarPubMed
Zampelas, A, Williams, CM, Morgan, LM & Wright, J (1994) The effect of triacylglycerol fatty acid positional distribution on postprandial plasma metabolite and hormone responses in normal adult men. British Journal of Nutrition 71, 401–410.CrossRefGoogle ScholarPubMed