Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-31T23:09:18.408Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of starch on the cariogenic potential of sucrose

Published online by Cambridge University Press:  08 March 2007

Cecilia C. C. Ribeiro ,
Cínthia P. M. Tabchoury ,
Altair A. Del Bel Cury ,
Livia M. A. Tenuta ,
Pedro L. Rosalen  and
Jaime A. Cury
Cecilia C. C. Ribeiro
Affiliation:
Faculty of Dentistry of Piracicaba, University of Campinas, Av. Limeira 901, CEP 13414-903, Piracicaba, São Paulo, Brazil
Cínthia P. M. Tabchoury
Affiliation:
Faculty of Dentistry of Piracicaba, University of Campinas, Av. Limeira 901, CEP 13414-903, Piracicaba, São Paulo, Brazil
Altair A. Del Bel Cury
Affiliation:
Faculty of Dentistry of Piracicaba, University of Campinas, Av. Limeira 901, CEP 13414-903, Piracicaba, São Paulo, Brazil
Livia M. A. Tenuta
Affiliation:
Faculty of Dentistry of Piracicaba, University of Campinas, Av. Limeira 901, CEP 13414-903, Piracicaba, São Paulo, Brazil
Pedro L. Rosalen
Affiliation:
Faculty of Dentistry of Piracicaba, University of Campinas, Av. Limeira 901, CEP 13414-903, Piracicaba, São Paulo, Brazil
Jaime A. Cury*
Affiliation:
Faculty of Dentistry of Piracicaba, University of Campinas, Av. Limeira 901, CEP 13414-903, Piracicaba, São Paulo, Brazil
*
*Corresponding author: Professor Jaime A. Cury, fax +55 19 3412 5218, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Since in vitro and animal studies suggest that the combination of starch with sucrose may be more cariogenic than sucrose alone, the study assessed in situ the effects of this association applied in vitro on the acidogenicity, biochemical and microbiological composition of dental biofilm, as well as on enamel demineralization. During two phases of 14 d each, fifteen volunteers wore palatal appliances containing blocks of human deciduous enamel, which were extra-orally submitted to four groups of treatments: water (negative control, T1); 2 % starch (T2); 10 % sucrose (T3); and 2 % starch+10 % sucrose (T4). The solutions were dripped onto the blocks eight times per day. The biofilm formed on the blocks was analysed with regard to amylase activity, acidogenicity, and biochemical and microbiological composition. Demineralization was determined on enamel by cross-sectional microhardness. The greatest mineral loss was observed for the association starch+sucrose (P<0·05). Also, this association resulted in the highest lactobacillus count in the biofilm formed (P<0·05). In conclusion, the findings suggest that a small amount of added starch increases the cariogenic potential of sucrose.

Keywords

StarchSucroseDental biofilmDemineralizationEnamel
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 1 , July 2005 , pp. 44 - 50
Copyright
Copyright © The Nutrition Society 2005

References

Bernfeld, P (1955) Amylases α and β. Meth Enzymol 1, 149–159.CrossRefGoogle Scholar
Bowen, WH, Amsbaugh, SM, Monell-Torrens, S, Brunelle, J, Kuzmiak-Jones, H & Cole, MF (1980) A method to assess cariogenic potential of foodstuffs. J Am Dent Assoc 100, 677–681.CrossRefGoogle ScholarPubMed
Bowen, WH, Pearson, S, Young, D & Thibodeau, E (1986) The effect of partial desalivation on coronal and root surface caries in the rat. In Factors Relating to Demineralization and Remineralization of the Teeth, pp.243–250 [Leach, SA, editor].Oxford: IRL Press.Google Scholar
Box, GEP, Hunter, WG & Hunter, JS (1978) Statistics for Experimenters. New York: John Wiley & Sons Inc.Google Scholar
Cury, JA, Hashizume, LN, Del Bel Cury, AA & Tabchoury, CPM (2001) Effects of dentifrice containing fluoride and/or baking soda on enamel demineralization/remineralization: an in situ study. Caries Res 35, 106–110.CrossRefGoogle ScholarPubMed
Cury, JA, Rebello, MA & Del Bel Cury, AA (1997) In situ relationship between sucrose exposure and the composition of dental plaque. Caries Res 31, 356–360.CrossRefGoogle ScholarPubMed
Cury, JA, Rebelo, MAB, Del Bel Cury, AA, Derbyshire, MTVC & Tabchoury, CPM (2000) Biochemical composition and cariogenicity of dental plaque formed in the presence of sucrose or glucose and fructose. Caries Res 34, 491–497.CrossRefGoogle ScholarPubMed
Dibdin, GH & Shellis, RP (1988) Physical and biochemical studies of Streptococcus mutans sediments suggest new factors linking the cariogenicity of plaque with its extracellular polysaccharide content. J Dent Res 67, 890–895.CrossRefGoogle ScholarPubMed
Dodds, MWJ & Edgar, WM (1986) Effects of dietary sucrose levels on pH fall and acid–anion profile in human dental plaque after a starch mouth-rinse. Arch Oral Biol 31, 509–512.CrossRefGoogle ScholarPubMed
Featherstone, JDB, ten Cate, JM, Shariati, M & Arends, J, (1983) Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. Caries Res 17, 385–391.CrossRefGoogle ScholarPubMed
Firestone, AR, Schmid, R & Mühlemann, HR (1982) Cariogenic effects of cooked wheat starch alone or with sucrose and frequency-controlled feedings in rats. Arch Oral Biol 27, 759–763.CrossRefGoogle ScholarPubMed
Fisher, FJ (1968) A field survey of dental caries, periodontal disease and enamel defects in Tristan da Cunha. Br Dent J 125, 447–453.Google ScholarPubMed
Gold, OG, Jordan, HV & Van Houte, J (1973) A selective medium for Streptococcus mutans. Arch Oral Biol 18, 1357–1364.CrossRefGoogle ScholarPubMed
Green, RM & Hartles, RL (1967) The effect of uncooked and roll-dried maize starch, alone and mixed in equal quantity with sucrose, on dental caries in the albino rat. Br J Nutr 21, 225–230.CrossRefGoogle ScholarPubMed
Gustaffson, BE, Quensel, CE, Lanke, LS, Lundqvist, C, Grahnen, H, Bonow, BE & Krasse, B (1954) The Vipeholm dental caries study; the effect of different levels of carbohydrate intake on caries activity in 436 individuals observed for five years. Acta Odontol Scand 11, 232–264.CrossRefGoogle Scholar
Hara, AT, Queiroz, CS, Paes Leme, AF, Serra, MC & Cury, JA (2003) Caries progression and inhibition in human and bovine root dentine in situ. Caries Res 37, 339–344.CrossRefGoogle ScholarPubMed
Hefti, A & Schmid, T (1979) Effect on caries incidence in rats of increasing dietary sucrose levels. Caries Res 13, 298–300.CrossRefGoogle ScholarPubMed
Imfeld, T (1977) Evaluation of the cariogenicity of confectionery by intra-oral wire-telemetry. SSO Schweiz Monatsschr Zahnheilkd 87, 437–464.Google ScholarPubMed
König, KG & Grenby, TH (1965) The effect of wheat grain fractions and sucrose mixtures on rat caries developing in two strains of rats maintained on different regimes and evaluated by two different methods. Arch Oral Biol 10, 143–153.CrossRefGoogle Scholar
Kopec, LK, Vacca-Smith, AM & Bowen, WH (1997) Structural aspects of glucans formed in solution and on the surface of hydroxyapatite. Glycobiology 7, 929–934.CrossRefGoogle ScholarPubMed
Larsen, MJ & Pearce, EI (1997) A computer program for correlating dental plaque pH values, cH +, plaque titration, critical pH, resting pH and the solubility of enamel apatite. Arch Oral Biol 42, 475–480.CrossRefGoogle ScholarPubMed
Lingström, P, Birkhed, D, Ruben, J & Arends, J (1994) Effect of frequent consumption of starchy food items on enamel and dentine demineralization and on plaque pH in situ. J Dent Res 73, 652–660.CrossRefGoogle ScholarPubMed
Lingström, P, Holm, J, Birkhed, D & Björck, I (1989) Effects of variously processed starch on pH of human dental plaque. Scand J Dent Res 97, 392–400.Google ScholarPubMed
Lingström, P, Van Houte, J & Kashket, S (2000) Food starches and dental caries. Crit Rev Oral Biol Med 11, 366–380.CrossRefGoogle ScholarPubMed
Marsh, PD (1994) Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res 8, 263–271.CrossRefGoogle ScholarPubMed
Marthaler, TM & Froesch, ER (1967) Deficient dental caries attack in persons with hereditary fructose intolerance. Hel Med Acta Suppl 47, 126.Google ScholarPubMed
Mattos-Graner, RO, Smith, DJ, King, WF & Mayer, MPA (2000) Water-insoluble glucan synthesis by mutans streptococcal strains correlates with caries incidence in 12- to 30-month-old children. J Dent Res 79, 1371–1377.CrossRefGoogle ScholarPubMed
Mattos-Graner, RO, Zelante, F, Line, RC & Mayer, M (1998) Association between caries prevalence and clinical, microbiological and dietary variables in 1·0 to 2·5-year-old Brazilian children. Caries Res 32, 319–323.CrossRefGoogle Scholar
Mundorff-Shrestha, SA, Featherstone, JD, Eisenberg, AD, Cowles, E, Curzon, ME, Espeland, MA & Shields, CP (1994) Cariogenic potential of foods. II. Relationship of food composition, plaque microbial counts, and salivary parameters to caries in the rat model. Caries Res 28, 106–115.CrossRefGoogle ScholarPubMed
Newbrun, E, Hoover, C, Mettraux, G & Graf, H (1980) Comparison of dietary habits and dental health of subjects with hereditary fructose intolerance and control subjects. J Am Dent Assoc 101, 619–626.CrossRefGoogle ScholarPubMed
Nobre, Dos Santos M, Melo, Dos Santos L, Francisco, SB & Cury, JA (2002) Relationship among dental plaque composition, daily sugar exposure and caries in the primary dentition. Caries Res 36, 347–352.CrossRefGoogle Scholar
Paes Leme, AF, Dalcico, R, Tabchoury, CP, Del Bel Cury, AA, Rosalen, PL & Cury, JA (2004) In situ effect of frequent sucrose exposure on enamel demineralization and on plaque composition after APF application and F dentifrice use. J Dent Res 83, 71–75.CrossRefGoogle ScholarPubMed
Pecharki, GD, Cury, JA, Paes Leme, AF, Tabchoury, CP, Del Bel Cury, AA, Rosalen, PL & Bowen, WH (2005) Effect of sucrose containing iron (II) on dental biofilm and enamel demineralization in situ. Caries Res 39, 123–129.CrossRefGoogle ScholarPubMed
Rölla, G, Scheie, AA & Ciardi, JE (1985) Role of sucrose in plaque formation. Scand J Dent Res 93, 105–111.Google ScholarPubMed
Stephan, RM (1940) Changes in hydrogen-ion concentration on tooth surfaces and in caries lesion. J Am Dent Assoc 27, 718–723.CrossRefGoogle Scholar
Vacca-Smith, AM, Venkitaraman, AR, Quivey, RG Jr & Bowen, WH (1996) Interactions of streptococcal glucosyltransferases with α-amylase and starch on the surface of saliva-coated hydroxyapatite. Arch Oral Biol 41, 291–298.CrossRefGoogle ScholarPubMed
White, DJ & Featherstone, JDB (1987) A longitudinal microhardness analysis of fluoride dentifrice effects on lesion progression in vivo. Caries Res 21, 502–512.CrossRefGoogle Scholar
Zylber, LJ & Jordan, H (1982) Development of a selective medium for detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. J Clin Microbiol 15, 253–259.CrossRefGoogle ScholarPubMed