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Effect of selenium deficiency on hepatic lipid and lipoprotein metabolism in the rat

Published online by Cambridge University Press:  09 March 2007

F. Nassir ,
C. Moundras ,
D. Bayle ,
C. Sérougne ,
E. Gueux ,
E. Rock ,
Y. Rayssiguier  and
A. Mazur
F. Nassir
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
C. Moundras
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
D. Bayle
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
C. Sérougne
Affiliation:
Laboratoire de Physiologie de la Nutrition, Université Paris Sud, 91405 Orsay, France
E. Gueux
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
E. Rock
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
Y. Rayssiguier
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
A. Mazur
Affiliation:
Centre de Recherche en Nutrition Humaine, Unité Maladies Métaboliques et Micronutriments, INRA Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France
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Abstract

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Since experimental Se deficiency results in a significant increase in plasma cholesterol concentration the present investigation was undertaken to assess further the influence of this deficiency on the expression of proteins involved in hepatic lipid metabolism. Se deficiency was induced by feeding weanling male Wistar rats on a deficient diet for 6 weeks. Hypercholesterolaemia associated with Se deficiency was related to increased 3-hydroxy-3-methylglutaryl-coA (HMG-CoA) reductase (EC 1.1.1.34) activity in liver microsomes as compared with control animals. Hepatic lipoprotein receptor levels (LDL-receptor and HDL-binding proteins, HB1 and HB2) were not significantly affected by Se deficiency, as assessed by immunoblotting. Plasma triacylglycerol concentrations tended to decrease in Se-deficient rats in concert with their reduced post-Triton secretion. There was no significant effect of Se deficiency on the hepatic synthesis of apolipoproteins. These results point to the need for further investigations into the mechanism related to the increased activity of HMG-CoA reductase and the enhanced cholesterogenesis in the liver of Se-deficient rats likely to result from this.

Selenium: Cholesterol: Triacylglycerol: HMG-CoA reductase

Keywords

SeleniumCholesterolTriacylglycerolHMG-CoA reductase
Type
General Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 3 , September 1997 , pp. 493 - 500
Copyright
Copyright © The Nutrition Society 1997

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