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The effect of protein and energy deficiency on skin glycosaminoglycan levels in the rat

Published online by Cambridge University Press:  09 March 2007

Jane S. N. Kaggwa
Jane S. N. Kaggwa
Affiliation:
MRC Dunn Nutrition Unit, Dunn Nutritional Laboratory, University of Cambridge and Medical Research Council, Milton Road, Cambridge CB4 1 XJ
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Abstract

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1. The effects of protein-deficient and energy-deficient diets on the concentrations of skin glycosaminoglycans (GAGs) and collagen were studied in young rats.

2. In Expt 1, comparisons were made between animals fed on a control (C) diet (protein:energy (P:E) 0.210), a low-protein (LP) diet (P:E 0.032) and a very-low-protein (VLP) diet (P:E 0005) ad lib. Skin GAGS and collagen concentrations were determined at intervals as the animals became malnourished and when some of those fed on the VLP diet started showing signs of oedema. Concurrently, plasma albumin concentrations were determined to assess the extent of hypoalbuminaemia. With prolonged severe protein deficiency all GAGS species were reduced below normal levels. Collagen concentration decreased with prolonged protein deficiency. Protein-deficient rats developed hypoalbuminaemia.

3. In Expt 2, comparisons were made between animals fed on the C, LP and VLP diets ad lib. and others given the C diet in restricted amounts sufficient to maintain body-weights identical to those fed on LP or VLP diets ad lib. Energy-deficient rats, wasting at rates similar to protein-deficient ones, had higher skin GAGs concentrations than those in protein-deficient or control animals. With prolonged malnutrition, collagen concentrations were similar in protein- and energy-deficient animals and lower than those observed in control animals. Plasma albumin concentrations in energy-deficient animals were above starting values, except in severely energy-deficient animals at the end of the experiment. Protein-deficient animals developed hypoalbuminaemia.

4. In conclusion, the present study has shown that the main difference between prolonged severe protein deficiency and energy deficiency in the rat is that skin hyaluronic acid concentration is higher than normal in energy deficiency but below normal levels in prolonged severe protein deficiency. Initially skin collagen concentrations are higher than normal in both protein- and energy-deficient animals. However, with prolonged malnutrition the concentrations are reduced.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 56 , Issue 2 , September 1986 , pp. 329 - 339
Copyright
Copyright © The Nutrition Society 1986

References

REFERENCES

Alleyne, G. A. O., Hay, R. W., Picou, D. I., Stanfield, J. P. & Whitehead, R. G. (1977). In Protein-Energy Malnutrition. London: Edward Arnold.Google Scholar
Anasuya, A. & Narasinga Rao, B. S. (1970). British Journal of Nutrition 24, 97–107.CrossRefGoogle Scholar
Angeleli, A. Y. O., Burini, R. C. & Campana, A. O. (1978). Journal of Nutrition 108 1147–1154.CrossRefGoogle Scholar
Arumugham, R. & Bose, S. M. (1979). Acta Biochimica Polonica 26, 295–301.Google Scholar
Bitter, T. & Muir, H. M. (1962). Analytical Biochemistry 4, 330–334.CrossRefGoogle Scholar
Cabak, V., Dickerson, J. W. T. & Widdowson, E. M. (1963). British Journal of Nutrition 17, 601–606.CrossRefGoogle Scholar
Coward, W. A., Whitehead, R. G. & Lunn, P. G. (1977). British Journal of Nutrition 38, 115–126.CrossRefGoogle Scholar
Halliday, D. (1967). Clinical Science 33, 365–370.Google Scholar
Kivirikko, K. I., Laitinen, O.&, Prockop, D. J. (1967). Analytical Biochemistry 19, 249–255.CrossRefGoogle Scholar
Newton, D. J., Scott, J. E. & Whiteman, P. (1974). Analytical Biochemistry 62, 268–273.CrossRefGoogle Scholar
Northam, B. E. & Widdowson, G. W. (1967). Association of Clinical and Biochemical Technical Bulletin 11, 1–6.Google Scholar
Picou, D., Halliday, D. & Garrow, J. S. (1966). Clinical Science 30, 345–351.Google Scholar
Scott, J. E. (1960). Methods in Biochemical Analysis 8, 145–197.CrossRefGoogle Scholar
Szirmai, J. A. (1970). In Advances in Biology of Skin, vol. 10, pp. 1–15. [Montagna, W., Bantley, J. p. and Dobson, R. L., editors]. New york: Appleton-Century-Crofts.Google Scholar
Vasantha, L. (1970). American Journal of Clinical Nutrition 23, 99–104.CrossRefGoogle Scholar
Vasantha, L., Srikantia, S. G. & Gopalan, C. (1970). American Journal of Clinical Nutrition 23, 78–82.CrossRefGoogle Scholar
Williams, C. D. (1935). Lancet ii, 1151–1152.CrossRefGoogle Scholar