Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-29T21:48:34.656Z Has data issue: false hasContentIssue false

The effect of ouabain on metabolic rate in guinea-pigs: estimation of energy cost of sodium pump activity

Published online by Cambridge University Press:  09 March 2007

R. Swaminathan
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Ellen L. P. CHAN
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Liu Yuet Sin
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Ng Siu King
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Ng Sui Fun
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Amy Y. S. Chan
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The effect of different doses of ouabain, an inhibitor of the sodium pump, or saline (9 g sodium chloride/1; the vehicle) on the metabolic rate of guinea-pigs weighing 500 g was measured by indirect calorimetry for 120 min.

2. Ouabain (0·02–0·07 nmol/g body-weight) decreased the oxygen consumption in a dose-related manner. When higher doses of ouabain (0·10 nmol/g body-weight or greater) were injected the animals were observed to shiver. Ouabain (0.07 nmol/g body-weight) reduced Na+, K+-ATPase (EC 3. 6. 1. 3) activity of liver, kidney and skeletal muscle by 18·0 (se 6·6), 21.5 (se 6·0) and 21·9 (se 6·8)% respectively. An Eadie-Hofstee plot of percentage decrease in O2 consumption v. percentage inhibition ÷ dose of ouabain showed that maximal inhibition of O2 consumption was 39·4 %.

3. It is concluded that Na pump activity contributed to about 40 % of the resting O2 consumption.

Type
Energy Metabolism
Copyright
Copyright © The Nutrition Society 1989

References

Arumanayagam, M., MacDonald, D. & Swaminathan, R. (1987). Differences in erythrocyte cation (sodium) transport between Chinese and non-Chinese males. Hypertension Clinical and Experimental A9, 719739.Google Scholar
Asano, Y., Liberman, U. A. & Edelman, I. S. (1976). Thyroid thermogenesis. Relationships between Na+- dependent respiration and Na+ + K+-adenosine triphosphatase activity in rat skeletal muscle. Journal of Clinical Investigation 57, 368379.CrossRefGoogle ScholarPubMed
Biron, R., Burger, A., Chinet, A., Clausen, T. & Dubois-Ferrière, R. (1979). Thyroid hormones and the energetics of active sodium-potassium transport in mammalian skeletal muscles. Journal of Physiology 297, 4760.CrossRefGoogle ScholarPubMed
Clark, D. G., Brinkman, M., Filsell, O. H., Lewis, S. J. & Berry, M. N. (1982). No major thermogenic role for (Na+ + K+)-dependent adenosine triphosphatase apparent in hepatocytes from hyperthyroid rats. Biochemical Journal 202, 661665.CrossRefGoogle Scholar
Daly, J. A. & Ertingshausen, G. (1972). Direct method for determining inorganic phosphate in serum with the ‘centrifichem’. Clinical Chemistry 18, 263265.CrossRefGoogle ScholarPubMed
Gregg, V. A. & Milligan, L. P. (1982). In vitro energy costs of Na+, K+-ATPase activity and protein synthesis in muscle from calves differing in age and breed. British Journal of Nutrition 48, 6571.CrossRefGoogle ScholarPubMed
Gstraunthaler, G., Pfaller, W. & Kotanko, P. (1985). Interrelation between oxygen consumption and Na-K-ATPase activity in rat renal proximal tubule suspension. Renal Physiology 8, 3844.Google ScholarPubMed
Guernsey, D. L. & Morishige, W. K. (1979). Na+ pump activity and nuclear T3 receptors in tissues of genetically obese (ob/ob) mice. Metabolism 28, 629632.CrossRefGoogle ScholarPubMed
Himms-Hagen, J. (1976). Cellular thermogenesis. Annual Review of Physiology 38, 315351.CrossRefGoogle ScholarPubMed
Kjeldson, K., Nørgaard, A., Hansen, O. & Clausen, T. (1985). Significance of skeletal muscle digitalis receptor for [3H]ouabain distribution in the guinea pig. Journal of Pharmacology and Experimental Therapeutics 234, 720727.Google Scholar
Lin, M. H., Romsos, D. R., Akera, T. & Leveille, G. A. (1981). Functional correlates of Na+, K+-ATPase in lean and obese (ob/ob) mice. Metabolism 30, 431438.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265275.CrossRefGoogle ScholarPubMed
McBride, B. W. & Milligan, L. P. (1985a). Magnitude of ouabain-sensitive respiration in the livers of growing, lactating and starved sheep. British Journal of Nutrition 54, 293303.CrossRefGoogle ScholarPubMed
McBride, B. W. & Milligan, L. P. (1985b). Influence of feed intake and starvation on the magnitude of Na+, K+-ATPase (EC 3. 6. 1. 3)-dependent respiration in duodenal mucosa of sheep. British Journal of Nutrition 53, 605614.CrossRefGoogle Scholar
Milligan, L. P. & McBride, B. W. (1985). Shifts in animal energy requirements across physiological and alimentational states. Energy costs of ion pumping by animal tissues. Journal of Nutrition 115, 13741382.CrossRefGoogle Scholar
Saddlier, S. & DeLuise, M. (1986). Mouse soleus muscle Na-K pump activity: direct correlation with in vitro and in vivo oxygen consumption. Hormone and Metabolic Research 18, 757760.CrossRefGoogle ScholarPubMed
Smith, T. J. & Edelman, I. S. (1979). The role of sodium transport in thyroid thermogenesis. Federation Proceedings 38, 21502153.Google ScholarPubMed
Swaminathan, R., Burrows, G. & McMurray, J. (1982). Energy cost of sodium pump activity in man: an in vivo study of metabolic rate in human subjects given digoxin. IRCS Medical Science 10, 949.Google Scholar
Sweadner, K. J. & Goldin, S. M. (1980). Active transport of sodium and potassium ions: mechanism, function and regulation. New England Journal of Medicine 302, 777783.CrossRefGoogle ScholarPubMed
Wardlaw, G. M. (1986). The effect of ouabain on basal and thyroid hormone-stimulated muscle oxygen consumption. International Journal of Biochemistry 18, 279281.CrossRefGoogle ScholarPubMed
Wollenberger, A. (1947). Metabolic action of cardiac glycoside. Influence on respiration of heart muscle and brain cortex. Journal of Pharmacology and Experimental Therapeutics 91, 3951.Google Scholar