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The effect of ouabain on metabolic rate in guinea-pigs: estimation of energy cost of sodium pump activity

Published online by Cambridge University Press:  09 March 2007

R. Swaminathan
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Ellen L. P. CHAN
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Liu Yuet Sin
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Ng Siu King
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Ng Sui Fun
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
Amy Y. S. Chan
Affiliation:
Department of Chemical Pathology, Prince of Wales Hospital, Shatin, N. T., Hong Kong
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Abstract

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1. The effect of different doses of ouabain, an inhibitor of the sodium pump, or saline (9 g sodium chloride/1; the vehicle) on the metabolic rate of guinea-pigs weighing 500 g was measured by indirect calorimetry for 120 min.

2. Ouabain (0·02–0·07 nmol/g body-weight) decreased the oxygen consumption in a dose-related manner. When higher doses of ouabain (0·10 nmol/g body-weight or greater) were injected the animals were observed to shiver. Ouabain (0.07 nmol/g body-weight) reduced Na+, K+-ATPase (EC 3. 6. 1. 3) activity of liver, kidney and skeletal muscle by 18·0 (se 6·6), 21.5 (se 6·0) and 21·9 (se 6·8)% respectively. An Eadie-Hofstee plot of percentage decrease in O2 consumption v. percentage inhibition ÷ dose of ouabain showed that maximal inhibition of O2 consumption was 39·4 %.

3. It is concluded that Na pump activity contributed to about 40 % of the resting O2 consumption.

Type
Energy Metabolism
Copyright
Copyright © The Nutrition Society 1989

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