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Effect of leucine at different levels of pyridoxine on hepatic quinolinate phosphoribosyl transferase (EC 2.4.2.19) and leucine aminotransferase (EC 2.6.1.6) in rats

Published online by Cambridge University Press:  24 July 2007

Kamala Krishnaswamy  and
S. Bapurao
Kamala Krishnaswamy
Affiliation:
National Institute of Nutrition, Indian Council of Medical Research, Jamai-Osmania, Hyderabad-500 007, India
S. Bapurao
Affiliation:
National Institute of Nutrition, Indian Council of Medical Research, Jamai-Osmania, Hyderabad-500 007, India
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Abstract

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1. Effects of incorporating 30 g leucine/kg into diets on quinolinate phosphoribosyl transferase (QPRT; EC 2.4.2.19) activity and leucine aminotransferase (EC 2.6.1.6) activity were studied in groups of rats receiving 5, 30 and 60 μg of pyridoxine/10 g diet.

2. The results indicated that 30 g leucine/kg diet significantly reduced the QPRT activity when the diets provided 5 μg pyridoxine/10 g and that the effect was only marginal when the diet included 30 μg pyridoxine/ 10 g. The inhibitory effect was completely absent when the diet provided higher amounts of pyridoxine (60 μg/10g).

3. These results suggest that additional amounts of pyridoxine are necessary to counteract the effects of excess of leucine in the diet.

4. Leucine aminotransferase activity was increased in rats given diets containing higher amounts of pyridoxine; supplementary leucine also increased the enzyme activity.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 39 , Issue 1 , January 1978 , pp. 61 - 64
Copyright
Copyright © The Nutrition Society 1978

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