Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-26T11:39:50.777Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of high intakes of casein and casein phosphopeptide on calcium absorption in the rat

Published online by Cambridge University Press:  09 March 2007

Teresa Bennett ,
Audrey Desmond ,
Maria Harrington ,
David McDonagh ,
Richard FitzGerald ,
Albert Flynn  and
Kevin D. Cashman
Teresa Bennett
Affiliation:
Department of Nutrition, University College, Cork, Ireland
Audrey Desmond
Affiliation:
Department of Nutrition, University College, Cork, Ireland
Maria Harrington
Affiliation:
Department of Nutrition, University College, Cork, Ireland
David McDonagh
Affiliation:
Dairy Quality Department, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland
Richard FitzGerald
Affiliation:
Life Science Department, University of Limerick, Limerick, Ireland
Albert Flynn
Affiliation:
Department of Nutrition, University College, Cork, Ireland
Kevin D. Cashman*
Affiliation:
Department of Nutrition, University College, Cork, Ireland
*
*Corresponding author: Dr Kevin Cashman, fax +353 21 270244, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effect of the level or source of dietary protein or protein-derived peptides on Ca absorption is not well understood. We determined, therefore, the influence of habitual dietary casein level, meal casein and meal casein phosphopeptide (CPP) on Ca absorption in the rat. True fractional Ca absorption was investigated in male 7-week-old rats, Wistar strain, in three separate studies using a faecal 47Sc : 47Ca ratio method. In studies A and C, rats (n 8 per group) were fed on a purified diet containing 200 g casein/kg for 2 weeks. Rats were then given a 47Ca-labelled meal (10 g) containing (per kg) either 0, 100, 200, or 300 g casein (study A) or 0, 100, 200, 350 or 500 g CPP (study C). In study B, rats (n 24 per group) were fed on a purified diet containing (per kg) either 200, 350 or 500 g casein for 2 weeks. Each group was then further randomized into three groups (n 8 per group) and given a 47Ca-labelled meal (10 g of the same diet) containing (per kg) either 200, 350 or 500 g casein. Ca absorption from a meal was unaffected by increasing meal casein concentration from 0 to 300 g/kg (study A), but was increased with a meal casein content of 500 g/kg (study B). Fractional Ca absorption decreased with increasing usual dietary casein intake in the range 200–500 g/kg (study B), suggesting intestinal adaptation. Ca absorption was unaffected by inclusion of 100 g CPP/kg in a single meal but was significantly (P < 0·001) reduced by 200, 350 and 500 g CPP/kg meal, with no evident dose-relationship. Thus, while Ca absorption was enhanced by high-casein meals, the mechanism remains unclear.

Keywords

Calcium absorptionCaseinCasein phosphopeptide
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 6 , June 2000 , pp. 673 - 680
Copyright
Copyright © The Nutrition Society 2000

References

Allen, LH and Hall, TE (1978) Calcium metabolism, intestinal calcium-binding protein, and bone growth of rats fed high protein diets Journal of Nutrition 108, 967–972.CrossRefGoogle ScholarPubMed
Allen, LHOddoye, EA and Margen, S (1979) Protein-induced hypercalciuria: a longer term study American Journal of Clinical Nutrition 32, 741–749.CrossRefGoogle ScholarPubMed
American Institute of Nutrition (1977) , Report of the American Institute ad hoc committee on standards for nutritional studies Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Berrocal, RChanton, SJuillerat, MAPavillard, BScherz, JC and Jost, R (1989) Tryptic phosphopeptides from whole casein. II. Physiochemical properties related to the solubilization of calcium Journal of Dairy Research 56, 335–341.CrossRefGoogle Scholar
Brommage, R & Binacua, C (1991) Intestinal calcium absorption varies during the rat estrous cycle. In Nutritonal Aspects of Osteoporosis, pp. 147–155 [Burckhardt, P & Heaney, RP, editors]. New York, NY: Raven Press.Google Scholar
Brommage, RJuillerat, MA and Jost, R (1991) Influence of casein phosphopeptides and lactulose on intestinal calcium absorption in adult female rats Lait 71, 173–180.CrossRefGoogle Scholar
Bronner, F (1987) Intestinal calcium absorption: Mechanisms and applications Journal of Nutrition 117, 1347–1352.CrossRefGoogle ScholarPubMed
Brule, G, Roger, L, Fauquant, J & Piot, M (1982) Casein phosphopeptide from casein-based material. US Patent no. 4,358,465.Google Scholar
Carrié, AL, Kastenmayer, P, Vigo, M & Binacua, C (1994) Influence of various proteins on intestinal calcium absorption in man. Ares-Serona Symposia Switzerland. Abstr.Google Scholar
Cashman, K and Flynn, A (1996) Effect of dietary calcium intake and meal calcium content on calcium absorption in the rat British Journal of Nutrition 76, 463–470.CrossRefGoogle ScholarPubMed
Hansen, MSandstrom, B, Jensen, M and Sørensen, SS (1997) Casein phosphopeptides improve zinc and calcium absorption from rice-based but not from whole-grain infant cereal Journal of Pediatric Gastroenterology and Nutrition 24, 56–62.Google Scholar
Hansen, MSandstrom, B, Jensen, M and Sørensen, SS (1997) Effect of casein phosphopeptides on zinc and calcium absorption from bread meals Journal of Trace Elements in Medicine and Biology 11, 143–149.CrossRefGoogle ScholarPubMed
Hansen, MSandstrom, B and Lonnerdal, B (1996) The effect of casein phosphopeptides on zinc and calcium absorption from high phytate infant diets assessed in rat pups and Caco-2 cells Pediatric Research 40, 547–552.CrossRefGoogle ScholarPubMed
Heaney, RPSaito, Y and Orimo, H (1994) Effect of casein phosphopeptides on absorbability of co-ingested calcium in normal postmenopausal women Journal of Bone Mineral Metabolism 12, 77–81.CrossRefGoogle Scholar
Hegsted, MSchuette, SAZemel, MB and Linkswiler, HM (1981) Urinary calcium and calcium balance in young men as affected by level of protein and phosphorus intake Journal of Nutrition 111, 553–562.CrossRefGoogle ScholarPubMed
Howe, JC and Beecher, GR (1981) Effect of dietary protein and phosphorus levels on calcium and phosphorus metabolism of the young growing rat Journal of Nutrition 111, 708–712.CrossRefGoogle Scholar
International Dairy Federation (1990) International Dairy Federation (IDF)-approved method for phosphorus determination (Provisional IDF Standard 42B: 1990).Google Scholar
International Dairy Federation (1993) International Dairy Federation (IDF)-approved Micro-Kjeldahl Method for protein determination (Provisional IDF Standard 20B: 1993).Google Scholar
Ireland, P and Fordtran, JS (1973) Effect of dietary calcium and age on jejunal calcium absorption in humans studied by intestinal perfusion Journal of Clinical Investigation 52, 2672–2681.CrossRefGoogle ScholarPubMed
Kim, Y and Linkswiler, HM (1979) Effect of level of protein intake on calcium metabolism and parathyroid and renal function in adult human male Journal of Nutrition 109, 1399–1404.CrossRefGoogle ScholarPubMed
Kitts, DD and Yuan, YV (1992) Caseinophosphopeptides and calcium bioavailability Trends in Food Science and Technology 3, 31–35.CrossRefGoogle Scholar
Kitts, DDYuan, YVNagasawa, T and Moriyama, Y (1992) Effect of casein, casein phosphopeptides and calcium intake on ileal 45Ca disappearance and temporal systolic blood pressure in spontaneously hypertensive rats British Journal of Nutrition 68, 765–781.CrossRefGoogle ScholarPubMed
Kopra, N, Scholz-Ahrens, KE & Barth, CA (1990) Influence of casein phosphopeptides on calcium bioavailability. Brief Communications of the XXIII International Dairy Congress, vol 1, pp. 184. Montreal, October 8–12.Google Scholar
Kopra, NScholz-Ahrens, KE and Barth, CA (1992) Effect of casein phosphopeptides on utilization of calcium in vitamin D-replete and vitamin D-deficient rats Milchwissenschaft 47, 488–493.Google Scholar
Lee, YSNoguchi, T and Naito, H (1979) An enhanced intestinal absorption of calcium in the rat directly attributed to dietary casein Agricultural and Biological Chemistry 43, 2009–2011.Google Scholar
Lee, YSNoguchi, T and Naito, H (1980) Phosphopeptides and soluble calcium in the small intestine of rat given a casein diet British Journal of Nutrition 43, 457–467.CrossRefGoogle Scholar
Lee, YSPark, G and Naito, H (1992) Effect of casein phosphopeptides (CPP) supplementation on the calcium balance of growing rats Nippon Eiyo Shokuryo Gakkaishi 4, 333–338.CrossRefGoogle Scholar
Li, YTomé, D and Desjeux, JF (1989) Indirect effect of casein phosphopeptides on calcium absorption in rat ileum in vitro Reproduction Nutrition Development 29, 227–233.CrossRefGoogle ScholarPubMed
McCredie, DA, Troehler, U and Bonjour, JP (1984) In vivo determination of intestinal calcium absorption, with scandium-47 used as a marker Journal of Laboratory and Clinical Medicine 103, 354–362.Google Scholar
McDonagh, D and FitzGerald, RJ (1998) Production of caseinophosphopeptides (CPPs) from sodium caseinate using a range of commercial protease preparations International Dairy Journal 8, 39–45.CrossRefGoogle Scholar
Mahalko, JRSandstead, HHJohnson, LK and Milne, DB (1983) Effect of a moderate increase in dietary protein on the retention and excretion of Ca, Cu, Fe, Mg, P, and Zn by adult males American Journal of Clinical Nutrition 37, 8–14.CrossRefGoogle ScholarPubMed
Mahoney, AW and Hendricks, DG (1978) Some effects of different compounds on iron and calcium absorption Journal of Food Science 43, 1473–1476.CrossRefGoogle Scholar
Mahoney, AW & Hendricks, DG (1985) Assaying calcium bioavailability in foods (applicability of the rat as a model). In Nutritional Bioavailability of Calcium, pp. 17–27 [Kies, C, editor]. Washington D.C.: American Chemical Society.Google Scholar
Mellander, O (1950) The physiologic importance of the casein phosphopeptide calcium salt, II: peroral calcium dosage of infants Acta Societae Medica (Uppsala) 55, 247–255.Google Scholar
Mellander, O and Olsson, N (1956) The influence of peptide bound calcium and phosphorus in bone calcification in rickets Biologica Medicus Del Hospital De Mexico 13, 243–246.Google ScholarPubMed
Miller, DD (1989) Calcium in the diet: Food sources, recommended intakes, and nutritional bioavailability Advances in Food and Nutrition Research 33, 103–156.CrossRefGoogle ScholarPubMed
Mykkanen, HM and Wasserman, RH (1980) Enhanced absorption of calcium by casein phosphopeptides in rachitic and normal chicks Journal of Nutrition 110, 2141–2148.CrossRefGoogle ScholarPubMed
Nagasawa, TYuan, YV and Kitts, DD (1991) Casein phosphopeptides enhance paracellular calcium absorption but do not alter temporal blood pressure in normotensive rats Nutrition Research 11, 819–830.CrossRefGoogle Scholar
Norman, AW (1990) Intestinal calcium absorption: a vitamin D-hormone mediated adaptive response American Journal of Clinical Nutrition 51, 290–300.CrossRefGoogle ScholarPubMed
Pannemans, DLESchaafsma, G and Westererp, KR (1997) Calcium excretion, apparent calcium absorption and calcium balance in young and elderly subjects: influence of protein intake British Journal of Nutrition 77, 721–729.CrossRefGoogle Scholar
Pansu, DBellaton, C and Bronner, F (1981) Effect of Ca intake on saturable and nonsaturable components of duodenal Ca transport American Journal of Physiology 240, G32–G37.Google ScholarPubMed
Pointillart, A and Guégen, L (1989) Effect of casein phosphopeptides on calcium and phosphorus utilization in growing pigs Reproduction Nutrition Development 29, 477–486.CrossRefGoogle Scholar
Saito, YLee, YS and Kimura, S (1998) Minimum effective dose of casein phosphopeptides (CPP) for enhancement of calcium absorption in growing rats International Journal of Vitamin and Nutrition Research 68, 335–340.Google ScholarPubMed
Sato, RNoguchi, T and Naito, H (1986) Casein phosphopeptide (CPP) enhances calcium absorption from the ligated segment of rat small intestine Journal of Nutritional Science and Vitaminology 32, 67–76.CrossRefGoogle ScholarPubMed
Scholz-Ahrens, KKopra, N and Barth, CA (1990) Effect of casein phosphopeptides on utilization of calcium in minipigs and vitamin-D-deficient rats Zeitschrift fur Ernahrungswissenschaft 29, 295–298.CrossRefGoogle ScholarPubMed
Schuette, SAZemel, MB and Linkswiler, HM (1980) Studies on the mechanism of protein induced hypercalciuria in older men and women Journal of Nutrition 110, 305–315.CrossRefGoogle ScholarPubMed
Shah, BGBelonje, B and Paquet, A (1990) The lack of effect of synthetic phosphoseryl peptide on calcium absorption by the rat Nutrition Research 10, 1331–1336.CrossRefGoogle Scholar
Shi, GLeray, LScarpignato, C, Bentouimou, NBruley des Varannes, SCherbut, C and Galmiche, JP (1997) Specific adaptation of gastric emptying to diets with differing protein content in the rat: is endogenous cholecystokinin implicated? Gut 41, 612–618.CrossRefGoogle ScholarPubMed
Snedecor, GW & Cochran, WG (1967) Statistical Methods. Ames: Iowa State University Press.Google Scholar
Spencer, HKramer, LDeBartolo, M, Norris, C and Osis, D (1983) Further studies of the effect of a high protein diet as meat on calcium metabolism American Journal of Clinical Nutrition 37, 924–929.CrossRefGoogle ScholarPubMed
Srinivasan, MR and Rao, MVL (1979) Availability of calcium and phosphate in calcium caseinate Journal of Food Science and Technology — Mysore 16, 95–99.Google Scholar
Tremaine, WJNewcomer, ADRiggs, BL and McGill, BD (1986) Calcium absorption from milk in lactase-deficient and lactase-sufficient adults Digestive Diseases and Sciences 31, 376–378.CrossRefGoogle ScholarPubMed
Tsuchita, HGoto, TShimizu, T, Yonehara, Y and Kuwata, T (1996) Dietary casein phosphopeptides prevent bone loss in aged ovariectomised rats Journal of Nutrition 126, 86–93.CrossRefGoogle Scholar
Tsuchita, HSekiguchi, IKuwata, T, Igarashi, C and Ezawaw, I (1993) The effect of casein phosphopeptides on calcium utilisation in young ovariectomised rats Zeitschrift fur Ernahrungswissenschaft 32, 121–130.CrossRefGoogle Scholar
Van der Meer, RDe Vries, HT and Van Tintelen, G (1988) The phosphorylation state of casein and the species-dependency of its hypercholesterolaemic effect British Journal of Nutrition 59, 467–473.CrossRefGoogle ScholarPubMed
Walker, RM and Linkswiler, HM (1972) Calcium retention in the adult human male as affected by protein intake Journal of Nutrition 102, 1297–1302.CrossRefGoogle ScholarPubMed
Whiting, SJ and Draper, HH (1980) The role of sulfate in the calciuria of high protein diets in adult rats Journal of Nutrition 110, 212–222.CrossRefGoogle ScholarPubMed
Yuan, YV and Kitts, DD (1991) Confirmation of calcium absorption and femoral utilization in spontaneously hypertensive rats fed casein phosphopeptide supplemented diets Nutrition Research 11, 1257–1272.CrossRefGoogle Scholar
Yuan, YV and Kitts, DD (1994) Calcium absorption and bone utilization in spontaneously hypertensive rats fed on native and heat-damaged casein and soyabean protein British Journal of Nutrition 71, 583–603.CrossRefGoogle Scholar
Zemel, B (1988) Calcium utilization: effect of varying level and source of dietary protein American Journal of Clinical Nutrition 102, 1297–1302.Google Scholar