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The effect of dietary guar gum on the activities of some key enzymes of carbohydrate and lipid metabolism in mouse liver

Published online by Cambridge University Press:  24 July 2007

John C. Stanley  and
Eric A. Newsholme
John C. Stanley
Affiliation:
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU
Eric A. Newsholme
Affiliation:
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU
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Abstract

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1. The effects of a 100 g/kg substitution of guar gum on the body-weight gain, food consumption and faecal dry weight of mice fed on a high-sucrose diet and on the activities of hepatic glucose-6-phosphate dehydrogenase (EC 1. 1. 1. 49), 6-phosphogluconate dehydrogenase (EC 1. I. 1. 44), malate dehydrogenase (oxaloacetate- decarboxylating) (NADP+) (EC I. 1. 1. 40), ATP-citrate (pro-3S)-lyase (EC 4. I.3.8), 6-phosphofructokinase (EC 2.7. 1. 11), pyruvate kinase (EC 2. 7. 1. 40)and fructose-1, Qbisphosphatase (EC 3. 1. 3. 11) were studied.

2. Guar gum had no effect on body-weight gain or food consumption but increased faecal dry weight.

3. Guar gum increasedtheactivitiesofglucose-6-phosphatedehydrogenase, malatedehydrogenase(oxaloacetate- decarboxylating) (NADP+) and 6-phosphofructokinase expressed on a wet-liver-weight basis.

4. Guar gum increased the activities of glucose-6-phosphate dehydrogenase, malate dehydrogenase (oxaloacetate- decarboxylating)(NADP+), ATP-citrate (pro-3S)-lyase and 6-phosphofructokinase expressed on a liver-protein basis.

5. Guar gum increased the activities of glucose-6-phosphate dehydrogenase and malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) expressed on a body-weight basis.

6. These results suggest that guar gum increases the flux through some pathways of hepatic lipogenesis when mice are fed on high-sucrose diets.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 53 , Issue 2 , March 1985 , pp. 215 - 222
Copyright
Copyright © The Nutrition Society 1985

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