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The effect of dietary calcium on the activity of 25-hydroxycholecalciferol-l-hydroxylase and Ca absorption in vitamin D-replete chicks

Published online by Cambridge University Press:  24 October 2018

R. Swaminathan ,
Barbara A. Sommerville  and
A. D. Care
R. Swaminathan
Affiliation:
Department of Animal Physiology and Nutrition, University of Leeds, Leeds LS2 9JF
Barbara A. Sommerville
Affiliation:
Department of Animal Physiology and Nutrition, University of Leeds, Leeds LS2 9JF
A. D. Care
Affiliation:
Department of Animal Physiology and Nutrition, University of Leeds, Leeds LS2 9JF
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Abstract

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  1. 1. As most of the studies on the regulation of renal 25-hydroxycholecalciferol-1-hydroxylase (25-HCC-1-hydroxylase) activity have been done in marginally-vitamin D-defieient animals and as it is known that vitamin D administration suppresses the specific activity of the 25-HCC-1-hydroxylase, it was decided to study the effect of dietary calcium on the activity of 25-HCC-1-hydroxylase and on Ca absorption in vitamin Dreplete chicks.

  2. 2. Chicks, 10 d old, were given diets differing in their Ca contents (65 nmol cholecalciferol/kg diet) for 10 d and the activity of 25-HCC-1-hydroxylase in kidney homogenates, Ca absorption from the duodenum, Cabinding protein (CaBP) activity in the duodenal mucosa and plasma Ca and phosphate concentrations were all determined.

  3. 3. The CaBP activity and the efficiency of Ca absorption both decreased with increasing dietary intake of Ca. Ca absorption and CaBP activity were significantly correlated (r 0.995, P < 0.01).

  4. 4. The activity of 25-HCC-1-hydroxylase decreased as the dietary level of Ca increased and was significantly correlated with Ca absorption (r0.900, P < 005). The plasma Ca concentration and the activity of 25-HCC-1-hydroxylase were inversely related (r-0.940, P < 0.01).

  5. 5. It is concluded that in the vitamin D-replete chick the efficiency of duodenal Ca absorption is regulated by the renal 25-HCC-1-hydroxylase activity via production of 1,25-dihydroxycholecalciferol and CaBP synthesis.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 38 , Issue 1 , July 1977 , pp. 47 - 54
Copyright
Copyright © The Nutrition Society 1977

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