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Effect of defaunation on the metabolism of rumen micro-organisms

Published online by Cambridge University Press:  09 March 2007

D. I. Demeyer  and
C. J. Van Nevel
D. I. Demeyer
Affiliation:
Laboratoriurn voor Voeding en Hygiene, Rijksuniversiteit Gent Proefhoevestraat, 10 B-9230 Melle, Belgium
C. J. Van Nevel
Affiliation:
Laboratoriurn voor Voeding en Hygiene, Rijksuniversiteit Gent Proefhoevestraat, 10 B-9230 Melle, Belgium
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Abstract

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1. Rumen contents of a fasted fistulated wether, obtained in a faunated, defaunated and refaunated period were incubated in vitro with a mixture of cellobiose and maltose, in the presence of ammonium bicarbonate and 32PO43−. Total synthesis of microbial N (Nt) was calculated from 32P incorporation and N:P determined in microbial matter. The N:P value was not affected by defaunation. Net synthesis of microbial N (Nn) was calculated from ammonia-N incorporation. An estimate of degradation of microbial N was calculated as Nt–Nn. Energetic efficiency of synthesis was calculated from the volatile fatty acids produced during incubation, as g N incorporated per kg organic matter fermented (g N/kg OMf).

2. Defaunation decreased the proportions of acetate, butyrate and methane and increased those of propionate in fermentation end-products. Fermentation rate when expressed per mg microbial N was not affected by defaunation.

3. Expressed per unit volume of rumen contents, Nn was increased by defaunation whereas Nf remained unchanged. Thus, a decrease in degradation can be calculated. Energetic efficiences of total and net synthesis were increased from 35 and 13 to 47 and 30 g N/kg OMf respectively.

4. Specific rates of both total and net synthesis of microbial N were significantly increased by defaunation whereas the specific rate of degradation was not affected.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 42 , Issue 3 , November 1979 , pp. 515 - 524
Copyright
Copyright © The Nutrition Society 1979

References

Abou Akkada, A. R. & El-Shazly, K. (1964). Appl. Microbiol. 12, 384.CrossRefGoogle Scholar
Bird, S. H. & Leng, R. A. (1978). Br. J. Nutr. 40, 163.CrossRefGoogle Scholar
Bryant, M. P. & Small, N. (1960). J. Dairy Sci. 43, 654.CrossRefGoogle Scholar
Campbell, R. C. (1967). Statistics for Biologists, p. 134. London: Cambridge University Press.Google Scholar
Christiansen, W. C., Kawashima, R. & Burroughs, W. (1965). J. Anim. Sci. 24, 730.CrossRefGoogle Scholar
Coleman, G. S. (1975). In Digestion and Metabolism in the Ruminant, p. 149 [McDonald, I. W. and Warner, A. C. I., editors]. Armidale, New South Wales: University of New England Publishing Unit.Google Scholar
Demeyer, D. I. & Henderickx, H. K. (1967). Biochem. J. 105, 271.CrossRefGoogle Scholar
Demeyer, D. I. & Van Nevel, C. J. (1975 a).In Digestion and Metabolism in the Ruminant, p. 366 [McDonald, I. W. and Warner, A. C. I., editors]. Armidale, New South Wales: University of New England Publishing Unit.Google Scholar
Demeyer, D. I. & Van Nevel, C. J. (1975 b). Misc. Papers, Landhouwhogeschool Wageningen 11, 31.Google Scholar
Eadie, J. M. & Gill, J. C. (1971). Br. J. Nutr. 26, 155.CrossRefGoogle Scholar
Eadie, J. M. & Hobson, P. N. (1962). Nature, Lond. 193. 503.CrossRefGoogle Scholar
Elias, A. (1972). PhD Thesis cited by P. Hobson, Proc. Nutr. Soc. 31, 135.Google Scholar
Grubb, J. A. & Dehority, B. A. (1976). Appl. Env. Microbiol. 31, 262.CrossRefGoogle Scholar
Herbert, D., Phipps, P. J. & Strange, R. E. (1971). Meth. Microbiol. 5B, 209.CrossRefGoogle Scholar
Hoogenraad, N. J. & Hird, F. J. R. (1970). J. gen. Microbiol. 62, 261.CrossRefGoogle Scholar
Hungate, R. E. (1966). The Rumen and its Microbes, p. 137. London and New York: Academic Press.Google Scholar
Hungate, R. E. (1969). Meth. Microbiol. 3B, 117.CrossRefGoogle Scholar
Hungate, R. E. (1972). Am. J. elin. Nutr. 25, 1480.CrossRefGoogle Scholar
Jarvis, B. D. W. (1968). Appl. Microbiol. 16, 714.CrossRefGoogle Scholar
Jouany, J. P. & Senaud, J. (1978). Annts Zoötech. 27, 61.CrossRefGoogle Scholar
Knight, R., Sutton, J. D., McAllan, A. B. & Smith, R. H. (1978). Proc. Nutr. Soc. 37, 14A.Google Scholar
Kurihara, Y., Eadie, J. M., Hobson, P. N. & Mann, S. O. (1968). J. gen. Microbiol. 51, 267.CrossRefGoogle Scholar
Lindsay, J. R. & Hogan, J. P. (1972). Aust. J. agric. Res. 23, 321.CrossRefGoogle Scholar
Males, J. R. & Purser, D. B. (1970). Appl. Microbiol. 19, 485.CrossRefGoogle Scholar
Marty, R. J. & Demeyer, D. I. (1973). Br. J. Nutr. 30, 369.CrossRefGoogle Scholar
Michailowski, T. & MusŻynski, P. (1978). J. agric. Sci., Camb. 90, 1.CrossRefGoogle Scholar
Nolan, J. V., Norton, W. B. & Leng, R. A. (1972). Tracer Studies on Norr-protein Nitrogenfor Ruminants p. 13. Vienna: International Atomic Energy Agency.Google Scholar
Orpin, C. G. (1977). J. appl. Bact. 43, 309.CrossRefGoogle Scholar
Paynter, M. J. B., Ewert, D. L. & Chalupa, W. (1969). Appl. Microbiol. 18, 942.CrossRefGoogle Scholar
Prins, R. A. (1967). Enkele microbiologische en biochemische aspecten van de stofwisseling in de pens. PhD Thesis, State University Utrecht, The Netherlands.Google Scholar
Prins, R. A. & van den Vorstenbosch, C. J. A. H. V. (1975). Misc. Papers Landbouwhogeschool Wageningen 11, 15.Google Scholar
Robinson, J. P. & Hungate, R. E. (1973). Int. J. Syst. Bact. 23, 171.CrossRefGoogle Scholar
Smith, R. H. & McAllan, A. B. (1974). Br. J. Nutr. 31, 27.CrossRefGoogle Scholar
Snedecor, G. W. (1955). Statistical Methods, p. 75. Ames, Iowa: Iowa State College Press.Google Scholar
Tempest, D. W., Herbert, D. & Phipps, P. J. (1967). In Microbial Physiolugy and Continuous Culture, p. 240 [Powell, E. O., Evans, C. G. T., Strange, R. E. and Tempest, D. W., editors]. London: H.M. Stationery Office.Google Scholar
Thomas, P. C. (1973). Proc. Nutr. Soc. 32, 85.CrossRefGoogle Scholar
Thompson, J. K. & Hobson, P. N. (1971). J. agric. Sci., Camb. 76, 423.CrossRefGoogle Scholar
Van Nevel, C. J. & Demeyer, D. I. (1977). Br. J. Nutr. 38, 101.CrossRefGoogle Scholar
Youssef, F. G. & Allen, D. M. (1968). Nature, Lond. 217, 777.CrossRefGoogle Scholar