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The effect of clenbuterol on basal protein turnover and endogenous nitrogen loss of sheep

Published online by Cambridge University Press:  09 March 2007

J. E. Inkster ,
F. D. Deb.Hovell ,
D. J. Kyle ,
D. S. Brown  and
G. E. Lobley
J. E. Inkster
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
F. D. Deb.Hovell
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
D. J. Kyle
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
D. S. Brown
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
G. E. Lobley
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

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Seven measurements of the effect of clenbuterol on basal nitrogen excretion (UNE), and protein turnover were made in six female sheep. The sheep were sustained by the intraruminal infusion of energy as volatile fatty acids to provide maintenance, but given no protein (N-free) for 12 d (6 d control, 6 d clenbuterol). Clenbuterol reduced UNE by 20 %, but only on day 2 of the 6 d subperiod. Protein flux (equivalent to degradation on N-free nutrition), measured on day 6 by the irreversible loss of leucine was significantly increased (12%) by clenbuterol. Amino-N oxidation measured by N excretion was unchanged and, therefore, protein synthesis was also increased. During the 12 d N-free period, the recovery of urinary total N (Kjeldahl) as the sum of urea, ammonia, creatinine and purine derivatives, declined from 87.7 to 74.2 %. The form of this missing N was not identified. The effect of clenbuterol of increasing both degradation and synthesis is unlike that reported in the literature for animals receiving protein when, in general, synthesis is unchanged and degradation reduced. This could be due to a different effect of clenbuterol in the N-free state, or to unchanged effects on protein pools other than muscle whose relative contribution to protein metabolism is different in the N-free state.

Keywords

Clenbuterol Nitrogen lossProtein turnoverNitrogen lossSheep
Type
Amino Acids and Proteins: Metabolism and Requirements
Information
British Journal of Nutrition , Volume 62 , Issue 2 , September 1989 , pp. 285 - 296
Copyright
Copyright © The Nutrition Society 1989

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