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Effect of ammonia concentration on rumen microbial protein production in vitro

Published online by Cambridge University Press:  24 July 2007

L. D Satter  and
L. L Slyter
L. D Satter
Affiliation:
Nutrition Institute, ARS, USDA, Beltsville, Maryland, USA
L. L Slyter
Affiliation:
Nutrition Institute, ARS, USDA, Beltsville, Maryland, USA
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Abstract

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1. The effect of ammonia concentration on microbial protein production was determined in continuous-culture fermentors charged with ruminal contents obtained from steers fed on either a protein-free purified diet, a maize-based all-concentrate diet, or a forage–concentrate (23:77) diet. Urea was infused into the fermentors to maintain various concentrations of ammonia in the incubating mixtures.

2. Under nitrogen-limiting conditions, microbial protein yield measured as tungstic acid-precipitable N (TAPN) increased linearly with supplementary urea until ammonia started to accumulate in the incubating ingesta. Increasing the ammonia concentration beyond 50 mg NH3-N/l had no effect on microbial protein production.

3. The molar proportions of volatile acids produced were not affected by the level of urea supplementation. Total acid production was decreased slightly under N-limiting conditions, but not to the same extent as microbial protein production.

4. Estimated yield of microbial dry matter/mol ATP produced averaged 15·6 when non-limiting N as urea was provided with the purified diet.

5. These results suggest that addition of non-protein N supplements to ruminant rations are warranted only if the prevailing concentration of ruminal ammonia is less than 50 mg NH3-N/l ruminal fluid.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 32 , Issue 2 , September 1974 , pp. 199 - 208
Copyright
Copyright © The Nutrition Society 1974

References

REFERENCES

Baldwin, R. L. (1970). Am. J. clin. Nutr. 23, 1508.CrossRefGoogle Scholar
Baurngardt, B. R. (1964). Wis. agric. exp. Stn Dairy Dep. Bull. No. 1.Google Scholar
Bryant, M. P. & Robinson, I. M. (1962). J. Bact. 84, 605.CrossRefGoogle Scholar
Campling, R. C., Freer, M. & Balch, C. C. (1962). Br. J. Nutr. 16, 115.CrossRefGoogle Scholar
Church, D. C. (1969). Digestive Physiology and Nutrition of Ruminants Vol. 1. Corvallis, Oregon: O.S.U. Book Stores Inc.Google Scholar
Cocimano, M. R. & Leng, R. A. (1967). Br. J. Nutr. 21, 353.CrossRefGoogle Scholar
Conway, E. J. (1950). Microdiffusion Analysis and Volumetric Error 2nd ed. London: Crosby and Lockwood.Google Scholar
Dehority, B. A., Johnson, R. R., Bentley, O. G. & Moxon, A. L. (1958). Archs Biochem. Biophys. 78, 15.CrossRefGoogle Scholar
Draper, N. R. & Smith, H. (1966). Applied Regression Analysis Ch. 5. New York: John Wiley and Sons Inc.Google Scholar
Esdale, W. J., Broderick, G. A. & Satter, L. D. (1968). J. Dairy Sci. 51, 1823.CrossRefGoogle Scholar
Henderson, C.Hobson, P. N. & Summers, R. (1969). In Continuous Cultivation of Microorganisms p. 189 [Malek, I, editor]. New York: Academic Press.Google Scholar
Hogan, J. P. & Weston, R. H. (1970). In Physiology of Digestion and Metabolism in the Ruminant p. 474 [Phillipson, A. T., editor] Newcastle upon Tyne: Oriel Press.Google Scholar
Hume, I. D., Moir, R. J. & Somers, M. (1970). Aust J. agric. Res. 21, 283.CrossRefGoogle Scholar
Hungate, R. E. (1965). In Physiology of Digestion in the Ruminant p. 314 [Dougherty, R. W., editor]. Washington, DC: Butterworts.Google Scholar
Hungate, R. E. (1966). The Rumen and Its Microbes Ch. 7. New York: Academic Press.Google Scholar
Oltjen, R. R., Slyter, L. L., Williams, E. E. jr & Kern, D. L. (1971). J. Nutr. 101, 101.CrossRefGoogle Scholar
Ørskov, E. R., Fraser, C. & McDonald, I (1971). Br. J. Nutr. 25, 243.CrossRefGoogle Scholar
Ørskov, E. R., Fraser, C. & McDonald, I. (1972). Br. J. Nutr. 27, 491.CrossRefGoogle Scholar
Shultz, T. A. & Shultz, E. (1970). J. Dairy Sci. 53, 781.CrossRefGoogle Scholar
Slyter, L. L., Kern, D. L., Weaver, J. M., Oltjen, R. R. & Wilson, R. L. (1971). J. Nutr. 101, 847.CrossRefGoogle Scholar
Slyter, L. L., Nelson, W. O. & Wolin, M. J. (1964). Appl. Microbiol. 12, 374.CrossRefGoogle Scholar
Slyter, L. L., Oltjen, R. R., Kern, D. L. & Weaver, J. M. (1968). J. Nutr. 94, 185.CrossRefGoogle Scholar
Slyter, L. L. & Putnam, P. A. (1967). J. Anim. Sci. 26, 1421.CrossRefGoogle Scholar
Walker, D. J. (1965). In Physiology of Digestion in the Ruminant Ch. 6 [Dougherty, R. W., editor]. Washington, DC: Buttenvorths.Google Scholar
Walker, D. J. & Nader, C. J. (1968). Appl. Microbiol. 16, 1124.CrossRefGoogle Scholar
Weston, R. H. (1967). Aust. J. agric. Res. 18, 983.CrossRefGoogle Scholar
Winter, K. A. & Pigden, W. J. (1971). Can. J. Anim. Sci. 51, 777.CrossRefGoogle Scholar