Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-26T16:42:43.077Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of acute carbohydrate load on the monophasic or biphasic nature of the postprandial lipaemic response to acute fat ingestion in human subjects

Published online by Cambridge University Press:  09 March 2007

Farideh Shishehbor ,
Helen M. Roche  and
Michael J. Gibney
Farideh Shishehbor
Affiliation:
Unit of Nutrition and Dietetics, Department of Clinical Medicine, Trinity Centre for Health Sciences, St James's Hospital, Dublin 8, Republic of Ireland
Helen M. Roche
Affiliation:
Unit of Nutrition and Dietetics, Department of Clinical Medicine, Trinity Centre for Health Sciences, St James's Hospital, Dublin 8, Republic of Ireland
Michael J. Gibney*
Affiliation:
Unit of Nutrition and Dietetics, Department of Clinical Medicine, Trinity Centre for Health Sciences, St James's Hospital, Dublin 8, Republic of Ireland
*
*Corresponding author:Professor Michael Gibney, fax +353 1 454 2043, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Previous studies in this laboratory have elicited a monophasic response in postprandial plasma triacylglycerol (TAG) level with fat intakes of 0.5 g fat/kg body weight accompanied by about 17 g carbohydrate as lactose. Recent studies involving the same level of fat with a higher level of carbohydrate, 136 g of which 60 g was sucrose, appeared to elicit a biphasic response. The present study compared these two test meals and showed a significant meal × time interaction for plasma total TAG (P = 0.0228) reflecting a monophasic response with the lower-carbohydrate test meal. The higher-carbohydrate meal induced significantly higher insulin and glucose-dependent insulinotropic polypeptide responses (P = 0.0009 and P = 0.0041 respectively). A significant meal × time interaction was seen for plasma non-esterified fatty acids (P = 0.0437). The biphasic plasma TAG response seen with the high-carbohydrate meal largely reflected the TAG-rich lipoprotein (TRL) or chylomicron fraction, which would tend to suggest a biphasic pattern of absorption. This was borne out by TRL-TAG fatty acid compositions. Both peaks in the biphasic response showed active incorporation of the main dietary fatty acids, 18:1n−9, 18:2n−6 and 18:3n−3 into TRL-TAG. These results indicate that under the specific test-meal conditions used in the present study, a biphasic pattern of fat absorption was seen.

Keywords

Postprandial metabolismCarbohydrateFat
Type
Research Article
Information
British Journal of Nutrition , Volume 80 , Issue 5 , November 1998 , pp. 411 - 418
Copyright
Copyright © The Nutrition Society 1998

References

Alam, MJ, Kerr, JI, Cormican, K & Buchanan, KD (1992) Gastric inhibitory polypeptide (GIP) response in diabetes using a highly specific antiserum. Diabetic Medicine 9, 542–545.CrossRefGoogle ScholarPubMed
Bergeron, H & Havel, RJ (1997) Assessment of postprandial lipaemia: nutritional influences. Current Opinion in Lipidology 8, 43–52.CrossRefGoogle ScholarPubMed
Cohen, JC & Schall, R (1988) Reassessing the effects of simple carbohydrates on the serum triglyceride responses to fat meals. American Journal of Clinical Nutrition 48, 1031–1034.CrossRefGoogle ScholarPubMed
Cohn, JS, McNamara, JR, Cohn, SD, Ordovas, JM & Schaefer, EJ (1988) Plasma apolipoprotein changes in the triglyceride-rich lipoprotein fraction of human subjects fed a fat-rich meal. Journal of Lipid Research 29, 925–936.CrossRefGoogle ScholarPubMed
Dubois, C, Armand, M, Azais–Braesco, V, Portugal, H, Pauli, A-M, Bernard, P-M, Latge, C, Lafont, H, Borel, P & Lairon, D (1994) Effects of moderate amounts of emulsified dietary fat on postprandial lipemia and lipoproteins in normo-lipidemic adults. American Journal of Clinical Nutrition 60, 374–382.CrossRefGoogle Scholar
Fielding, BA, Callow, J, Owen, RM, Samra, JM, Matthews, DR & Frayn, KN (1996) Postprandial lipaemia: the origin of an early peak studied by specific dietary fatty acid intake during sequential meals. American Journal of Clinical Nutrition 63, 36–41.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Stanley, GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Grant, KI, Marais, MP & Dhansay, MA (1994) Sucrose in a lipid-rich meal amplifies the postprandial excursion of serum and lipoprotein triglyceride and cholesterol concentration by decreasing triglyceride clearance. American Journal of Clinical Nutrition 59, 853–860.CrossRefGoogle Scholar
Grundy, SM & Mok, HYI (1976) Chylomicron clearance in normal and hyperlipidemic men. Metabolism 25, 1225–1239.CrossRefGoogle Scholar
Jeppesen, J, Chen, Y-DI, Zhou, M-Y, Schaaf, P, Coulston, A & Reaven, GM (1995) Postprandial triglyceride and retinyl ester responses to oral fat: effects of fructose. American Journal of Clinical Nutrition 61, 787–791.CrossRefGoogle ScholarPubMed
Kashyap, ML, Barnhurt, RL, Srivastava, LS, Perisutti, G, Allen, C, Hogg, E, Glueck, CJ & Jackson, RL (1983) Alimentary lipemia: plasma high-density lipoproteins and apolipoproteins CII and CIII in healthy subjects. American Journal of Clinical Nutrition 37, 233–243.CrossRefGoogle ScholarPubMed
Knapper, JME, Morgan, LM & Fletcher, JM (1996) Nutrient-induced secretion and metabolic effects of glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1. Proceedings of the Nutrition Society 55, 291–305.CrossRefGoogle ScholarPubMed
Mann, JI, Truswell, AS & Pimstone, BL (1971) Different effects of oral sucrose and glucose on alimentary lipaemia. Clinical Science 41, 123–129.CrossRefGoogle ScholarPubMed
Mattes, RD (1996) Oral fat exposure alters postprandial lipid metabolism in humans. American Journal of Clinical Nutrition 63, 911–917.Google ScholarPubMed
Matthews, JNS, Altman, DG, Campbell, MJ & Royston, P (1990) Analysis of serial measurements in medical research. British Medical Journal 300, 230–235.CrossRefGoogle ScholarPubMed
Mendeloff, AL (1954) The effects of eating and sham feeding upon the absorbance of vitamin A palmitate in man. Journal of Clinical Investigation 33, 1015–1021.CrossRefGoogle Scholar
Murphy, MC, Isherwood, S, Sethi, S, Gould, BJ, Wright, JW, Knapper, JA & Williams, CM (1995) Postprandial lipid and hormone responses to meals of varying fat contents: modulatory role of lipoprotein lipase? European Journal of Clinical Nutrition 49, 579–588.Google ScholarPubMed
Olefsky, JM, Crapo, P & Reaven, GM (1976) Postprandial plasma triglyceride and cholesterol responses to a low fat meal. American Journal of Clinical Nutrition 29, 535–539.CrossRefGoogle ScholarPubMed
Peel, AS, Zampelas, A, Williams, CM & Gould, BJ (1993) A novel antiserum specific to apolipoprotein B-48: application in the investigation of postprandial lipidaemia in humans. Clinical Science 85, 521–524.CrossRefGoogle ScholarPubMed
Roche, HM & Gibney, MJ (1995) Postprandial triacylglycerolaemia – nutritional implications. Progress in Lipid Research 34, 249–266.CrossRefGoogle ScholarPubMed
Roche, HM & Gibney, MJ (1996) Postprandial triacylglycerolaemia: the effect of low-fat dietary treatment with and without fish oil supplementation. European Journal of Clinical Nutrition 50, 617–624.Google ScholarPubMed
Roche, HM & Gibney, MJ (1997) Postprandial coagulation factor VII activity: the effect of monounsaturated fatty acids. British Journal of Nutrition 77, 537–549.CrossRefGoogle ScholarPubMed
Roche, HM, Zampelas, A, Jackson, KG, Williams, CM & Gibney, MJ (1998 a) The effect of test meal MUFA:SFA ratio on postprandial lipid metabolism. British Journal of Nutrition 79, 419–424.CrossRefGoogle Scholar
Roche, HM, Zampelas, A, Knapper, JME, Webb, D, Brooks, C, Jackson, KG, Wright, JW, Gould, BJ, Kafatos, A, Gibney, MJ & Williams, CM (1998 b) Effect of long-term olive oil dietary intervention on postprandial triacylglyerol and factor VII metabolism. American Journal of Clinical Nutrition (In the Press).CrossRefGoogle Scholar
Sethi, S, Gibney, MJ & Williams, CM (1993) Postprandial lipoprotein meabolism. Nutrition Research Reviews 6, 161–183.CrossRefGoogle Scholar
Van Amelsvoort, JMM, Van Stratum, P, Kraal, JH, Lussenburg, RN & Houtsmuller, UMT (1989) Effects of varying the carbohydrate: fat ratio in a hot lunch on postprandial variables in male volunteers. British Journal of Nutrition 61, 267–283.CrossRefGoogle Scholar
Van Greevenbroek, MMJ, van Meer, G, Erkelens, DW & de Bruin, TWA (1996) Effects of saturated, mono- and polyunsaturated fatty acids on the secretion of apo B containing lipoprotein by Caco-2 cells. Atherosclerosis 121, 139–150.CrossRefGoogle ScholarPubMed
Zampelas, A, Peel, AS, Gould, BJ & Williams, CM (1994) Polyunsaturated fatty acids of the n−6 and n−3 series: effects on postprandial lipid and lipoprotein levels in healthy men. European Journal of Clinical Nutrition 48, 842–848.Google ScholarPubMed
Zampelas, A, Roche, HM, Kapsolefalou, M, Knapper, JME, Jackson, KG, Pentaris, E, Tornaritis, M, Hatzis, C, Gibney, MJ, Kafatos, A, Gould, BJ, Wright, J & Williams, CM (1998) Differences in postprandial lipaemic responses, but not traditional plasma lipid coronary risk indicators between Northern and Southern Europeans. Atherosclerosis (In the Press).CrossRefGoogle ScholarPubMed