Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-05T04:55:23.979Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of a methionine-supplemented diet on the blood pressure of Sprague–Dawley and deoxycorticosterone acetate–salt hypertensive rats

Published online by Cambridge University Press:  09 March 2007

Sophie Robin ,
Véronique Maupoil ,
Pascal Laurant ,
Alain Jacqueson  and
Alain Berthelot
Sophie Robin*
Affiliation:
Laboratoire de Physiologie, Pharmacologie et Nutrition Préventive Expérimentale, UFR Médecine et Pharmacie, 4 Place Saint-Jacques, 25030, Besançon, cedex, France
Véronique Maupoil
Affiliation:
Laboratoire de Physiologie, Pharmacologie et Nutrition Préventive Expérimentale, UFR Médecine et Pharmacie, 4 Place Saint-Jacques, 25030, Besançon, cedex, France
Pascal Laurant
Affiliation:
Laboratoire de Physiologie, Pharmacologie et Nutrition Préventive Expérimentale, UFR Médecine et Pharmacie, 4 Place Saint-Jacques, 25030, Besançon, cedex, France
Alain Jacqueson
Affiliation:
Laboratoire de Toxicologie, UFR Médecine et Pharmacie, 4 Place Saint-Jacques, 25030, Besançon, cedex, France
Alain Berthelot
Affiliation:
Laboratoire de Physiologie, Pharmacologie et Nutrition Préventive Expérimentale, UFR Médecine et Pharmacie, 4 Place Saint-Jacques, 25030, Besançon, cedex, France
*
*Corresponding author: Dr Sophie Robin, fax +33 3 81 66 56 91, email [email protected].
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The objectives of the present study were to evaluate the effects of a methionine-supplemented diet on systolic blood pressure (BP) and vasomotor functions in Sprague–Dawley (SD) and deoxycorticosterone acetate (DOCA)–salt hypertensive rats. SD and DOCA rats were fed a normal or a methionine (8 g/kg)-supplemented diet for 10 weeks. Systolic BP was monitored and plasma homocysteine, methionine and cysteine levels were determined at the end of the experiment. Vasoconstriction and vasodilatation of aortic rings were measured. The methionine-supplemented diet induced a greater increase in homocysteinaemia concentration in DOCA rats than in SD rats and an increase in plasma cysteine concentration in DOCA rats. This diet was associated with an increase in systolic BP in SD rats and with a lesser development of DOCA–salt hypertension. An enhanced aortic constriction and a decreased responsiveness to acetylcholine, bradykinin and sodium nitroprusside in the SD rats fed the methionine-rich diet were consistent with the elevated systolic BP. In DOCA rats the increased responsiveness to bradykinin was in accordance with the systolic BP-lowering effect. In conclusion, the methionine-enriched diet cannot simply be considered as model of hyperhomocysteinaemia, since other metabolites and mechanisms seemed to be implicated in these complex interactions. The differential vasopressive effect of the methionine supplementation in SD and DOCA rats, and in particular the lowering of systolic BP obtained with a greater degree of hyperhomocysteinaemia in DOCA rats, suggest that more complex interactions exist between hyperhomocysteinaemia and BP than the simple positive association described previously.

Keywords

Deoxycorticosterone acetate–salt hypertensive ratsHomocysteineAortic rings
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 6 , June 2004 , pp. 857 - 865
Copyright
Copyright © The Nutrition Society 2004

References

Charpiot, P, Bescond, A, Augier, T, Chareyre, C, Fraterno, M, Rolland, PH & Garcon, D (1998) Hyperhomocysteinemia induces elastolysis in minipig arteries: structural consequences, arterial site specificity and effect of captopril-hydrochlorothiazide. Matrix Biol 17, 559–574.CrossRefGoogle ScholarPubMed
Fiorina, P, Lanfredini, M, Montanari, A, Peca, MG, Veronelli, A, Mello, A, Astorri, E & Craveri, A (1998) Plasma homocysteine and folate are related to arterial blood pressure in type 2 diabetes mellitus. Am J Hypertens 11, 1100–1107.CrossRefGoogle ScholarPubMed
Hanratty, CG, McGrath, LT, McAuley, DF, Young, IS & Johnston, GD (2001) The effects of oral methionine and homocysteine on endothelial function. Heart 85, 326–330.CrossRefGoogle ScholarPubMed
Kanani, PM, Sinkey, CA, Browning, RL, Allaman, M, Knapp, HR & Haynes, WG (1999) Role of oxidant stress in endothelial dysfunction produced by experimental hyperhomocyst(e)inemia in humans. Circulation 100, 1161–1168.CrossRefGoogle ScholarPubMed
McCully, KS (1969) Vascular pathology of homocysteinemia: implications for the pathogenesis of arteriosclerosis. Am J Pathol 56, 111–128.Google ScholarPubMed
Majors, A, Ehrhart, LA & Pezacka, EH (1997) Homocysteine as a risk factor for vascular disease enhanced collagen production and accumulation by smooth muscle cells. Arterioscler Thromb Vasc Biol 17, 2074–2081.CrossRefGoogle ScholarPubMed
Mansoor, MA, Bergmark, C, Haswell, SJ, Savage, IF, Evans, PH, Berge, RK, Svardal, AM & Kristensen, O (2000) Correlation between plasma total homocysteine and copper in patients with peripheral vascular disease. Clin Chem 46, 385–391.CrossRefGoogle ScholarPubMed
Matthias, D, Becker, CH, Riezler, R & Kindling, PH (1996) Homocysteine induced arterosclerosis-like alterations of the aorta in normotensive and hypertensive rats following application of high doses of methionine. Atherosclerosis 122, 201–216.CrossRefGoogle ScholarPubMed
Militante, JD & Lombardini, JB (2002) Treatment of hypertension with oral taurine: experimental and clinical studies. Amino Acids 23, 381–393.CrossRefGoogle ScholarPubMed
Millette, E, de Champlain, J & Lamontagne, D (2000) Altered coronary dilatation in deoxycorticosterone acetate-salt hypertension. J Hypertens 18, 1783–1793.CrossRefGoogle ScholarPubMed
Nakata, Y, Katsuya, T, Takami, S, Sato, N, Fu, Y, Ishikawa, K, Takiuchi, S, Rakugi, H, Miki, T, Higaki, J & Ogihara, T (1998) Methylenetetrahydrofolate reductase gene polymorphism: relation to blood pressure and cerebrovascular disease. Am J Hypertens 11, 1019–1023.CrossRefGoogle ScholarPubMed
Pinto, Y, Buikema, H, Van Gilst, WH, Scholtens, E, Van Geel, PP, de Graeff, PA, Wagner, J & Paul, M (1997) Cardiovascular end-organ damage in Ren-2 transgenic rats compared to spontaneously hypertensive rats. J Mol Med 75, 371–377.CrossRefGoogle ScholarPubMed
Robin, S, Maupoil, V, Groubatch, F, Laurant, P, Jacqueson, A & Berthelot, A (2003) Effect of a methionine-supplemented diet on the blood pressure of Wistar–Kyoto and spontaneously hypertensive rats. Br J Nutr 89, 539–548.CrossRefGoogle ScholarPubMed
Rolland, PH, Friggi, A, Barlatier, A, Piquet, P, Latrille, V, Faye, MM, Guillou, J, Charpiot, P, Bodard, H & Ghiringhelli, O (1995) Hyperhomocysteinemia-induced vascular damage in the minipig. Circulation 91, 1161–1174.CrossRefGoogle ScholarPubMed
Stockand, JD, Zeltwanger, S, Bao, HF, Becchetti, A, Worrell, RT & Eaton, DC (2001) S -adenosyl- l -homocysteine hydrolase is necessary for aldosterone-induced activity of epithelial Na(+) channels. Am J Physiol Cell Physiol 281, C773–C785.CrossRefGoogle ScholarPubMed
Sutton-Tyrrell, K, Bostom, A, Selhub, J & Zeigler-Johnson, C (1997) High homocysteine levels are independently related to isolated systolic hypertension in older adults. Circulation 96, 1745–1749.CrossRefGoogle ScholarPubMed
Taylor, LM Jr, Moneta, GL, Sexton, GJ, Schuff, RA & Porter, JM (1999) Prospective blinded study of the relationship between plasma homocysteine and progression of symptomatic peripheral arterial disease. J Vasc Surg 29, 8–19.CrossRefGoogle ScholarPubMed
Ungvari, Z, Pacher, P, Rischak, K, Szollar, L & Koller, A (1999) Dysfunction of nitric oxide mediation in isolated rat arterioles with methionine diet-induced hyperhomocysteinemia. Arterioscler Thromb Vasc Biol 19, 1899–1904.CrossRefGoogle ScholarPubMed
Yen, CH & Lau, YT (2002) Vascular responses in male and female hypertensive rats with hyperhomocysteinemia. Hypertension 40, 322–328.CrossRefGoogle ScholarPubMed