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Docosahexaenoic acid enhances the antioxidant response of human fibroblasts by upregulating γ-glutamyl-cysteinyl ligase and glutathione reductase

Published online by Cambridge University Press:  08 March 2007

Khelifa Arab ,
Adrien Rossary ,
Françoise Flourié ,
Yves Tourneur  and
Jean-Paul Steghens
Khelifa Arab
Affiliation:
UF 21455 Oxidative Stress and Vitamins,Biochemistry Federation, E. Herriot Hospital, Lyon, France EA 3090, Claude Bernard University Lyon 1, France
Adrien Rossary
Affiliation:
UF 21455 Oxidative Stress and Vitamins,Biochemistry Federation, E. Herriot Hospital, Lyon, France EA 3090, Claude Bernard University Lyon 1, France
Françoise Flourié
Affiliation:
UF 21455 Oxidative Stress and Vitamins,Biochemistry Federation, E. Herriot Hospital, Lyon, France
Yves Tourneur
Affiliation:
Quantimetry Center, Pharmacy Faculty, Claude Bernard University Lyon 1, France
Jean-Paul Steghens*
Affiliation:
UF 21455 Oxidative Stress and Vitamins,Biochemistry Federation, E. Herriot Hospital, Lyon, France EA 3090, Claude Bernard University Lyon 1, France
*
*Corresponding author: Dr Jean-Paul Steghens, fax +33 4 72 11 06 75, email [email protected]
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Abstract

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The chemopreventive effects of dietary n-3 PUFA in various pathologies has so far remained controversial, and we were interested in studying their potential influence on cell redox status. DHA (22:6n-3), a typical highly unsaturated n-3 PUFA, was used at 30µmol/l in a model of human fibroblast cell culture. A dose–response effect, roughly linear, was checked for DHA between 0 and 60µmol/l, and was accompanied by a large increase in intracellular GSH content. A time course study of this effect shows that, after a short fall, as soon as 4h after the beginning of the experiment, the large increase in the GSH content was associated with elevated catalytic activities of γ-glutamyl-cysteinyl ligase, glutathione reductase and glutathione S-transferase. This coordinated response is characteristic of an antioxidant response and was confirmed by the induction of expression of mRNA for γ-glutamyl-cysteinyl ligase, glutathione reductase and haem-oxygenase. This large increase in the GSH content contributes to decreasing the reactive oxygen species level, as assessed by the decreased accumulation of dichlorofluorescein inside cells. To our knowledge, this is the first report on a specific and potent effect of DHA for decreasing the oxidative stress of human fibroblasts.

Keywords

Docosahexaenoic acidChemopreventionThiol-redox statusFibroblastsGlutathioneOxidative stress
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 1 , January 2006 , pp. 18 - 26
Copyright
Copyright © The Nutrition Society 2006

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