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Disposition kinetics and dosage regimen of vitamin E administered intramuscularly to sheep

Published online by Cambridge University Press:  09 March 2007

M. Hidiroglou  and
K. Karpinski
M. Hidiroglou
Affiliation:
Animal Research Centre, Agriculture Canada, Central Experimental Farm, Ottawa, Ontario KI A 0C6, Canada
K. Karpinski
Affiliation:
Drugs Directorate, Health and Welfare, Banting Research Building, Ottawa, Ontario KIA 0C6, Canada
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Abstract

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Three experiments were conducted to estimate the effects of single intramuscular (IM) administrations of vitamin E on blood plasma and tissue concentrations of α-tocopherol in sheep. In Expt 1, plasma kinetics of α-tocopherol in sheep (n 30) were investigated following IM administration of three doses (ten sheep/dose) of DL-α-tocopheryl acetate, (20, 40 and 60 mg/kg live weight). Plasma profiles of α-tocopherol consisted of a lag phase followed by an apparent first-order absorption and elimination phase. The rate of absorption and elimination, as well as the lag phase, were independent of the dose, but the extent of absorption was directly proportional to dose. In Expt 2 (eighteen experimental and five control sheep), the animals were injected as in Expt 1 and were killed at 0, 80 and 176 h. Increases in α-tocopherol levels in organs were much higher than in plasma. Some tissues such as liver, spleen, lung and adrenal appeared to exhibit rapid absorption and elimination phases. The amount absorbed was proportional to the dose administered. Other organs such as heart, kidney and pancreas had a slow elimination rate. In Expt 3, D-α-tocopherol was injected IM into ten sheep at either 604 mg or 1208 mg. The mean hepatic α-tocopherol concentrations in both groups rose rapidly and after 4 weeks of dosing its concentrations were higher than the predosing levels. The increase in hepatic tocopherol concentrations were higher following 1208 mg dosing than 604 mg D-α-tocopherol. No simple relationship existed between plasma and hepatic α-tocopherol concentrations. This suggests a difference in body mechanisms controlling vitamin E in blood and liver.

Keywords

Intramuscular vitamin ESheep
Type
Vitamin Metabolism
Information
British Journal of Nutrition , Volume 65 , Issue 3 , May 1991 , pp. 465 - 473
Copyright
Copyright © The Nutrition Society 1991

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