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Differential effects of n-3 and n-6 polyunsaturated fatty acids on BRCA1 and BRCA2 gene expression in breast cell lines

Published online by Cambridge University Press:  09 March 2007

Dominique J. Bernard-Gallon ,
Cécile Vissac-Sabatier ,
David Antoine-Vincent ,
Pascale G. Rio ,
Jean-Claude Maurizis ,
Pierre Fustier  and
Yves-Jean Bignon
Dominique J. Bernard-Gallon
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
Cécile Vissac-Sabatier
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
David Antoine-Vincent
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
Pascale G. Rio
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France INSERM U 484, 63005 Clermont-Ferrand, France
Jean-Claude Maurizis
Affiliation:
INSERM U 484, 63005 Clermont-Ferrand, France
Pierre Fustier
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
Yves-Jean Bignon*
Affiliation:
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58, rue Montalembert - B.P. 392 - 63011 Clermont-Ferrand Cedex 1, France
*
*Corresponding author: Dr Yves-Jean Bignon, fax +33 4 73 27 80 42, email [email protected]
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Abstract

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Current evidence strongly supports a role for the breast tumour suppressor genes, BRCA1 and BRCA2, in both normal development and carcinogenesis. In vitro observations reported that BRCA1 and BRCA2 are expressed in a cell cycle-dependent manner. Interestingly, differences in the actions of n-3 and n-6 polyunsaturated fatty acids have been observed: while the n-3 polyunsaturated fatty acids have been described to reduce pathological cell growth, the n-6 polyunsaturated fatty acids have been found to induce tumour proliferation. Here, we examined the expression of BRCA1 and BRCA2 in breast cell lines after treatment with polyunsaturated fatty acids. Real-time quantitative polymerase chain reaction determinations conclusively demonstrated increases in BRCA1 and BRCA2 mRNA expressions in MCF7 and MDA-MB 231 tumour cell lines after treatment with n-3 polyunsaturated fatty acids (eicosapentaenoic acid and docosahexaenoic acid), but no variation was noticed with the n-6 polyunsaturated fatty acid (arachidonic acid). On the other hand, no variation of the expression of BRCA1 and BRCA2 mRNA was detected in MCF10a normal breast cell line treated by polyunsaturated fatty acids. The level of BRCA1 and BRCA2 proteins quantified by affinity chromatography remained unchanged in tumour (MCF7, MDA-MB 231) and normal (MCF10a) breast cell lines. We suggest the presence of a possible transcriptional or post-transcriptional regulation of BRCA1 and BRCA2 after n-3 polyunsaturated fatty acid treatment in breast tumour cells.

Keywords

BRCA1BRCA2Polyunsaturated fatty acidsMammary cell linesReal-time quantitative polymerase chain reaction
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 4 , April 2002 , pp. 281 - 289
Copyright
Copyright © The Nutrition Society 2002

References

Albino, AP, Juan, G, Traganos, F, Reinhart, L, Connolly, J, Rose, DP & Darzynkiewicz, Z (2000) Cell cycle arrest and apoptosis of melanoma cells by docosahexaenoic acid: association with decreased pRb phosphorylation. Cancer Research 60, 4139–4145.Google ScholarPubMed
Beck, SA, Smith, KL & Tisdale, MJ (1991) Anticachectic and antitumor effect of eicosapentaenoic acid and its effect on protein turnover. Cancer Research 51, 6089–6093.Google ScholarPubMed
Bernard-Gallon, DJ, Maurizis, JC, Rio, PG, Bougnoux, P & Bignon, YJ (1998) Effects of monounsaturated and polyunsaturated fatty acids (omega-3 and omega-6) on BRCA1 protein expression in breast cell lines. Journal of the National Cancer Institute 90, 1234–1235.CrossRefGoogle ScholarPubMed
Bieche, I, Nogues, C & Lidereau, R (1999) Overexpression of BRCA2 gene in sporadic breast tumours. Oncogene 18, 5232–5238.CrossRefGoogle ScholarPubMed
Blagosklonny, MV, An, WG, Melillo, G, Nguyen, P, Trepel, JB & Neckers, LM (1999) Regulation of BRCA1 by protein degradation. Oncogene 18, 6460–6468.CrossRefGoogle ScholarPubMed
Brouard, C & Pascaud, M (1993) Modulation of rat and human lymphocyte function by n-6 and n-3 polyunsaturated fatty acids and acetylsalicylic acid. Annals of Nutritional Metabolism 37, 146–159.CrossRefGoogle ScholarPubMed
Cailleau, R, Young, R, Olive, M & Reeves, WJ Jr (1974) Breast tumor cell lines from pleural effusions. Journal of the National Cancer Institute 53, 661–674.CrossRefGoogle ScholarPubMed
Danesch, U, Weber, PC & Sellmayer, A (1996) Differential effects of n-6 and n-3 polyunsaturated fatty acids on cell growth and early gene expression in Swiss 3T3 fibroblasts. Journal of Cellular Physiology 168, 618–624.3.0.CO;2-V>CrossRefGoogle ScholarPubMed
Deng, CX & Brodie, SG (2000) Roles of BRCA1 and its interacting proteins. Bioessays 22, 728–737.3.0.CO;2-B>CrossRefGoogle ScholarPubMed
Dobrovic, A & Simpfendorfer, D (1997) Methylation of the BRCA1 gene in sporadic breast cancer. Cancer Research 57, 3347–3350.Google ScholarPubMed
Favy, DA, Lafarge, S, Rio, P, Vissac, C, Bignon, YJ & Bernard-Gallon, D (2000) Real-time PCR quantification of full-length and exon 11 spliced BRCA1 transcripts in human breast cancer cell lines. Biochemical and Biophysical Research Communications 274, 73–78.CrossRefGoogle ScholarPubMed
Fink, L, Seeger, W, Ermert, L, Hanze, J, Stahl, U, Grimminger, F, Kummer, W & Bohle, RM (1998) Real-time quantitative RT-PCR after laser-assisted cell picking. Nature Medicine 4, 1329–1333.CrossRefGoogle ScholarPubMed
Galli, C & Marangoni, F (1997) Recent advances in the biology of n-6 fatty acids. Nutrition 13, 978–985.CrossRefGoogle ScholarPubMed
Grammatikos, SI, Subbaiah, PV, Victor, TA & Miller, WM (1994) n-3 and n-6 fatty acid processing and growth effects in neoplastic and non-cancerous human mammary epithelial cell lines. British Journal of Cancer 70, 219–227.CrossRefGoogle ScholarPubMed
Hizel, C, Maurizis, JC, Rio, P, Communal, Y, Chassagne, J, Favy, D, Bignon, YJ & Bernard-Gallon, DJ (1999) Isolation, purification and quantification of BRCA1 protein from tumour cells by affinity perfusion chromatography. Journal of Chromatography B 721, 163–170.CrossRefGoogle ScholarPubMed
Magdinier, F, Ribieras, S, Lenoir, GM, Frappart, L & Dante, R (1998) Down-regulation of BRCA1 in human sporadic breast cancer; analysis of DNA methylation patterns of the putative promoter region. Oncogene 17, 3169–3176.CrossRefGoogle ScholarPubMed
Mancini, DN, Rodenhiser, DI, Ainsworth, PJ, O'Malley, FP, Singh, SM, Xing, W & Archer, TK (1998) CpG methylation within the 5′ regulatory region of the BRCA1 gene is tumor specific and includes a putative CREB binding site. Oncogene 16, 1161–1169.CrossRefGoogle ScholarPubMed
Miki, Y, Swensen, J, Shattuck-Eidens, D, Futreal, PA, Harshman, K, Tavtigian, S, Liu, Q, Cochran, C, Bennett, LM & Ding, W (1994) A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science 266, 66–71.CrossRefGoogle Scholar
Rice, JC, Massey-Brown, KS & Futscher, BW (1998) Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17, 1807–1812.CrossRefGoogle ScholarPubMed
Rose, DP & Connolly, JM (1989) Stimulation of growth of human breast cancer cell lines in culture by linoleic acid. Biochemical and Biophysical Research Communications 164, 277–283.CrossRefGoogle ScholarPubMed
Rose, DP & Connolly, JM (1990) Effects of fatty acids and inhibitors of eicosanoid synthesis on the growth of a human breast cancer cell line in culture. Cancer Research 50, 7139–7144.Google ScholarPubMed
Rose, DP & Connolly, JM (1991) Effects of fatty acids and eicosanoid synthesis inhibitors on the growth of two human prostate cancer cell lines. Prostate 18, 243–254.CrossRefGoogle ScholarPubMed
Sellmayer, A, Uedelhoven, WM, Weber, PC & Bonventre, JV (1991) Endogenous non-cyclooxygenase metabolites of arachidonic acid modulate growth and mRNA levels of immediate-early response genes in rat mesangial cells. Journal of Biological Chemistry 266, 3800–3807.CrossRefGoogle ScholarPubMed
Shiina, T, Terano, T, Saito, J, Tamura, Y & Yoshida, S (1993) Eicosapentaenoic acid and docosahexaenoic acid suppress the proliferation of vascular smooth muscle cells. Atherosclerosis 104, 95–103.CrossRefGoogle ScholarPubMed
Skehan, P, Storeng, R, Scudiero, D, Monks, A, McMahon, J, Vistica, D, Warren, JT, Bokesch, H, Kenney, S & Boyd, MR (1990) New colorimetric cytotoxicity assay for anticancer-drug screening. Journal of the National Cancer Institute 82, 1107–1112.CrossRefGoogle ScholarPubMed
Soule, HD, Maloney, TM, Wolman, SR, Peterson, WD Jr, Brenz, R, McGrath, CM, Russo, J, Pauley, RJ, Jones, RF & Brooks, SC (1990) Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Research 50, 6075–6086.Google ScholarPubMed
Soule, HD, Vazguez, J, Long, A, Albert, S & Brennan, M (1973) A human cell line from a pleural effusion derived from a breast carcinoma. Journal of the National Cancer Institute 51, 1409–1416.CrossRefGoogle ScholarPubMed
Thakur, S, Zhang, HB, Peng, Y, Le, H, Carroll, B, Ward, T, Yao, J, Farid, LM, Couch, FJ, Wilson, RB & Weber, BL (1997) Localization of BRCA1 and a splice variant identifies the nuclear localization signal. Molecular and Cellular Biology 17, 444–452.CrossRefGoogle Scholar
Thompson, ME, Jensen, RA, Obermiller, PS, Page, DL & Holt, JT (1995) Decreased expression of BRCA1 accelerates growth and is often present during sporadic breast cancer progression. Nature Genetics 9, 444–450.CrossRefGoogle ScholarPubMed
Tisdale, MJ & Dhesi, JK (1990) Inhibition of weight loss by omega-3 fatty acids in an experimental cachexia model. Cancer Research 50, 5022–5026.Google Scholar
Vissac, C, Antoine-Vincent, D, Maurizis, J-C, Bignon, Y-J & Bernard-Gallon, DJ (2001) Determination of BRCA2 oncosuppressor protein expression in human mammary cells by affinity perfusion high-performance chromatography. Journal of Biochemical and Biophysical Methods 49, 657–663.CrossRefGoogle ScholarPubMed
Welsch, CW, Oakley, CS, Chang, CC & Welsch, MA (1993) Suppression of growth by dietary fish oil of human breast carcinomas maintained in three different strains of immune-deficient mice. Nutrition and Cancer 20, 119–127.CrossRefGoogle ScholarPubMed
Whitehouse, AS, Smith, HJ, Drake, JL & Tisdale, MJ (2001) Mechanism of attenuation of skeletal muscle protein catabolism in cancer cachexia by eicosapentaenoic acid. Cancer Research 61, 3604–3609.Google ScholarPubMed
Wickens, M, Anderson, P & Jackson, RJ (1997) Life and death in the cytoplasm: messages from the 3′ end. Current Opinion in Genetics and Development 7, 220–232.CrossRefGoogle Scholar
Wilson, CA, Payton, MN, Elliott, GS, Buaas, FW, Cajulis, EE, Grosshans, D, Ramos, L, Reese, DM, Slamon, DJ & Calzone, FJ (1997) Differential subcellular localization, expression and biological toxicity of BRCA1 and the splice variant BRCA1-delta11b. Oncogene 14, 1–16.CrossRefGoogle ScholarPubMed
Wooster, R, Bignell, G, Lancaster, J, Swift, S, Seal, S, Mangion, J, Collins, N, Gregory, S, Gumbs, C & Micklem, G (1995) Identification of the breast cancer susceptibility gene BRCA2. Nature 378, 789–792.CrossRefGoogle ScholarPubMed