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Different effects of whole milk and a fermented milk with the same fat and lactose content on gastric emptying and postprandial lipaemia, but not on glycaemic response and appetite

Published online by Cambridge University Press:  09 March 2007

K. M. Sanggaard ,
J. J. Holst ,
J. F. Rehfeld ,
B. Sandström ,
A. Raben  and
T. Tholstrup
K. M. Sanggaard
Affiliation:
Research Department of Human Nutrition, the Royal Veterinary and Agricultural University, DK-1958 Frederiksberg, Denmark
J. J. Holst
Affiliation:
Department of Medical Physiology, The Panum Institute, University of Copenhagen, DK-2400 Copenhagen N, Denmark
J. F. Rehfeld
Affiliation:
Department of Clinical Biochemistry, Rigshospitalet, DK-2100 Copenhagen, Denmark
B. Sandström
Affiliation:
Research Department of Human Nutrition, the Royal Veterinary and Agricultural University, DK-1958 Frederiksberg, Denmark
A. Raben
Affiliation:
Research Department of Human Nutrition, the Royal Veterinary and Agricultural University, DK-1958 Frederiksberg, Denmark
T. Tholstrup*
Affiliation:
Research Department of Human Nutrition, the Royal Veterinary and Agricultural University, DK-1958 Frederiksberg, Denmark
*
*Corresponding author: fax +45 35 28 69, Email [email protected]
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Abstract

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Longitudinal studies indicate that milk and fermented milk products lower basal plasma cholesterol concentrations, despite their high content of saturated fat, and therefore have favourable health effects. However, there have been few studies on the postprandial effects of milk products. The present study compared the effect of whole milk with a fermented milk, A-38, on postprandial carbohydrate and lipid metabolism, gastric emptying and appetite. Eight healthy young men participated. On the two test days, they arrived fasting for collection of baseline values before consuming the meals, which for a 75 kg subject consisted of 1·4 litre milk or fermented milk, plus 165 mg [13C]acetate (for later determination of gastric emptying by a [13C]acetate breath test). Lactose (15 g) was added to the A-38 meal to equalize the lactose content. Postprandially the A-38 meal resulted in a slower gastric emptying rate than milk (P>0·001). Furthermore, the A-38 meal resulted in a greater increase and a quicker decrease of the triacylglycerol content in all lipoprotein fractions (LDL-fraction, P>0·05; other fractions, P>0·001) and of the gastrointestinal hormones (cholecystokinin and peptide YY, P>0·05; gastric inhibitory polypeptide and glucagon-like polypeptide-1, P>0·001). There were no significant differences in appetite sensations (measured by visual analogue scale) or in the glucose and insulin response (P<0·10). The slower emptying rate of the liquid phase after the A-38 meal is probably due to the higher viscosity of A-38. The lower and more prolonged triacylglycerol response after the milk meal might be caused by coagulation of milk in the stomach.

Keywords

MilkFermented milkGastric emptying rateHungerSatietyLipid metabolismInsulinGlucose
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 3 , September 2004 , pp. 447 - 459
Copyright
Copyright © The Nutrition Society 2004

References

Agerbæk, M, Gerdes, LU & Richelsen, BHypocholesterolaemic effect of a new fermented milk product in healthy middle-aged men. Eur J Clin Nutr (1995) 49, 346–352.Google ScholarPubMed
Aitken, RCBMeasurement of feelings using visual analogue scales. Proc R Soc Med (1969) 62, 989–993.Google ScholarPubMed
Andersen, L, Dinesen, B, Jørgensen, PN, Poulsen, F & Røder, MEEnzyme immunoassay for intact human insulin in serum or plasma. Clin Chem (1993) 39, 578–582.CrossRefGoogle ScholarPubMed
Austin, MATriacylglyerol and coronary heart disease. Proc Nutr Soc (1997) 56, 667–670.CrossRefGoogle Scholar
Bergmann, JF, Chassany, O, Petit, A, Triki, R, Caulin, C & Segrestaa, JMCorrelation between echographic gastric emptying and appetite: influence of psyllium. Gut (1992) 33, 1042–1043.CrossRefGoogle ScholarPubMed
Braden, B, Adams, S, Duan, LP, Orth, KH, Maul, FD, Lembcke, B, Hör, G & Caspary, WFThe [ 13 C]acetate breath test accurately reflects gastric emptying of liquids in both liquid and semisolid test meals. Gastroenterology (1995) 108, 1048–1055.CrossRefGoogle ScholarPubMed
Clark, PMS & Hales, CNAssay of insulin. In Textbook of Diabetes, vol.1, 335–347 [JC, Pickup and G, Williamseditors]. Oxford: Blackwell Scientific Publications. (1991)Google Scholar
Collins, PJ, Houghton, LA, Read, NW, Horowitz, M, Chatterton, BE, Heddle, R & Dent, JRole of the proximal and distal stomach in mixed solid and liquid meal emptying. Gut (1991) 32, 615–619.CrossRefGoogle ScholarPubMed
Cortot, A, Phillips, SF & Malagelada, JRGastric emptying of lipids after ingestion of solid-liquid meal in humans. Gastroenterology (1981) 80, 922–927.CrossRefGoogle ScholarPubMed
Croset, M, Brossard, N, Lecerf, J, Pachiaudi, C, Normand, S, Chirouze, V, Riou, JP, Tayot, JL & Lagarde, MUtilization of stable isotopes to study the compartment metabolism of polyunsaturated fatty acids: in vivo study using [13CO2]- docosahexaenoic acid. In New Trends in Lipid and Lipoprotein Analyses pp.309–316 [JC, Sebedio and EG, Perkins, editors]. Champaign, IL: AOCS Press (1995)Google Scholar
Cullen, JJ & Kelly, KAGastric motor physiology and pathophysiology. Surg Clin North Am (1993) 73, 1145–1160.CrossRefGoogle ScholarPubMed
Deacon, CF, Nauck, MA, Meier, J, Hücking, K & Holst, JJDegradation of endogenous and exogenous gastric inhibitory polypeptide (GIP) in healthy and in type 2 diabetic subjects as revealed using a new assay for the intact peptide. J Clin Endocrinol Metab (2000) 85, 3575–3581.Google ScholarPubMed
Douglas, BR, Jansen, JBMJ, de Jong, AJL & Lamers, BHWEffect of various triglycerides on plasma cholecystokinin levels in rats. J Nutr (1990) 120, 686–690.CrossRefGoogle ScholarPubMed
Douglas, BR, Woutersen, RA, Jansen, JBMJ, de Jong, AJL & Lamers, CBHWThe influence of different nutrients on plasma cholecystokinin levels in the rat. Experientia (1988) 44, 21–22.CrossRefGoogle ScholarPubMed
Drøhse, HB & Foltmann, BSpecificity of milk-clotting enzymes towards bovine κ-casein. Biochim Biophys Acta (1989) 995, 221–224.CrossRefGoogle ScholarPubMed
Ebihara, K, Miyada, T & Mochizuki, SComparative effects of various organic acids on glucose-flattening activity in rats fed a glucose solution. Nutr Rep Int (1989) 40, 1041–1047.Google Scholar
Eckel, RH, Fujimoto, WY & Brunzell, JDGastric inhibitory polypeptide enhanced lipoprotein lipase activity in cultured preadipocytes. Diabetes (1979) 28, 1141–1142.CrossRefGoogle ScholarPubMed
Edelbroek, M, Horowitz, M, Maddox, A & Bellen, JGastric emptying and intragastric distribution of oil in the presence of a liquid or a solid meal. J Nucl Med (1992) 33, 1283–1290.Google ScholarPubMed
Eggstein, M & Kuhlmann, ETriglycerides and glycerol Determination after alkaline hydrolysis. In Methods of Enzymatic Analysis,2nd English ed. pp.1825–1831 [HU, Bergmeyereditor]. London and New York: Verlag Chemie Weinheim and Academic Press. (1974)Google Scholar
Ehrlein, HJ & Pröve, JEffect of viscosity of test meals on gastric emptying. Q J Exp Physiol (1982) 67, 419–425.CrossRefGoogle ScholarPubMed
Eichholzer, M & Stähelin, HIs there a hypocholesterolemic factor in milk and milk products? Int J Vitam Nutr Res (1993) 63, 159–167Google Scholar
Flint, A, Raben, A, Blundell, JE & Astrup, AReproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes (2000) 24 28–48CrossRefGoogle ScholarPubMed
Foltmann, BChymosin: a short review on foetal and neonatal gastric proteases. Scand J Clin Lab Invest (1992) 210 Suppl., 65–79CrossRefGoogle ScholarPubMed
Foltmann, BPepsin, chymosin and their related zymogens.In Molecular and Cellular Basis of Digestion 491–505 [Desnuelle, P, Sjöström, H and Norén, O, editors]. Amsterdam, New York, Oxford: Elsevier (1986)Google Scholar
Frayn, KNInsulin resistence and lipid metabolism. Curr Opin Lipidol (1993) 4 197–204.CrossRefGoogle Scholar
Gallati, H & Pracht, IPeroxidase aus meerrettich: kinetische studien und optimierung der peroxidase-aktivitätsbestimmung mit den substraten H2O2 und 3,3′5,5′-tetramethyl-benzidin (Horseradish peroxidase: kinetic studies and optimization of the peroxidase activity determination with the substrates H2O2 and 3,3′5,5′-tetramethylbenzidine). J Clin Chem Clin Biochem (1985) 23 453–460.Google Scholar
Ganong, WFRegulation of gastrointestinale function. In Review of Medical Physiology, 15th ed. 448–476East Norwalk, CT, USA: Prentice-Hall International Inc (1991)Google Scholar
Havel, R (1994) McCollum award lecture, 1993: triglyceride-rich lipoproteins and artherosclerosis-new prospectives. Am J Clin Nutr (1994) 59 795–799CrossRefGoogle Scholar
Havel, RJ (1997) Postprandial lipid metabolism: an overview Proc Nutr Soc 56 659–666CrossRefGoogle ScholarPubMed
Hill, AJ, Magson, LD & Blundell, JEHunger and palatability: tracking ratings of subjective experience before, during and after the consumption of preferred and less preferred food. Appetite (1984) 5 361–371CrossRefGoogle ScholarPubMed
Holst, JJGLP-1in NIDDM. Diabet Med (1996) 13 S156–S160CrossRefGoogle Scholar
Holst, JJ & Bersani, MAssays for peptide products of somatostatin gene expression. In Methods in Neuroscience, 5, 3–22 [Conn, PM, editor]. San Diego, CA: Academic Press. (1991)Google Scholar
Holt, S, Brand, J, Soveny, C & Hansky, JRelations of satiety to postprandial glycaemic, insulin and cholecystokinin responses. Appetite (1992) 18 129–141CrossRefGoogle ScholarPubMed
Houghton, LA, Read, NW, Heddle, R, Horowitz, M, Collins, PJ, Chatterton, B & Dent, JRelationship of the motor activity of the antrum, pylorus and duodenum to gastric emptying of a solid-liquid mixed meal. Gastroenterology (1988) 94, 1285–1291.CrossRefGoogle ScholarPubMed
Hunt, JN, Smith, JL & Jiang, CLEffect of meal volumen and energy density on gastric emptying of carbohydrates. Gastroenterology (1985) 89, 1326–1330.CrossRefGoogle Scholar
Jacobsen, BK & Stensvold, IMilk – a better drink?. Scand J Soc Med (1992) 20, 204–208.CrossRefGoogle ScholarPubMed
Jones, KL, Doran, SM, Hveem, K, Bartholomeusz, FDL, Morley, JE, Sun, WMS, Chatterton, BE & Horowitz, MRelation between postprandial satiation and antral area in normal subjects. Am J Clin Nutr (1997) 66, 127–132.CrossRefGoogle ScholarPubMed
Kaiyala, KJ, Woods, SC & Schwartz, MWNew model for the regulation of energy balance and adiposity by the central nerveous system. Am J Clin Nutr (1995) 62, Suppl.1123S–1134S.CrossRefGoogle Scholar
Karpe, FPostprandial lipid metabolism in relation to conorary heart disease. Proc Nutr Soc (1997) 56, 671–678.CrossRefGoogle Scholar
Kattermann, R, Jaworek, D, & Möller, G et al. . Multicentre study of a new enzymatic method of cholesterol determination. J Clin Chem Clin Biochem (1984) 22, 245–251.Google ScholarPubMed
Keys, ACoronary heart disease in seven countries. Circulation (1970) 41, 1–211.Google Scholar
Kostner, GM, Molinari, E & Pichler, PEvaluation of a new HDL2/HDL3 quantitation method based on precipation with polyethyleneglycol. Clin Chim Acta (1985) 148, 139–147.CrossRefGoogle Scholar
Krarup, T, Madsbad, S, Moody, AJ, Regeur, L, Faber, OK & Holst, JJDiminished immunoreactive gastric inhibitory polypeptide response to a meal in newly diagnosed type 1 (insulin-dependent) diabetes. J Clin Endocrinol Metab (1983) 56, 1306–1312.CrossRefGoogle Scholar
Lavin, JH & Read, NWThe effect on hunger and satiety of slowing the absorption of glucose: relationship with gastric emptying and postprandiel blood glucose and insulin responses. Appetite (1995) 25, 89–96.CrossRefGoogle ScholarPubMed
Liljeberg, HGM & Björck, IMEDelayed gastric emptying rate as a potential mechanism for lowered glycemia after eating sourdough bread: studies in humans and rats using test products with added organic acids or an organic salt. Am J Clin Nutr (1996) 64, 886–893.CrossRefGoogle ScholarPubMed
Mann, GV & Spoerry, AStudies of a surfactant and cholesteremia in the Maasai. Am J Clin Nutr (1974) 27, 464–469.CrossRefGoogle ScholarPubMed
Mata, P, Garrido, JA, Ordovas, JM, Blazquez, E, Alvarez-Sala, LA, Rubio, MJ, Alonso, R & de Oya, MEffect of dietary monounsaturated fatty acids on plasma lipoproteins and apolipoproteins in woman. Am J Clin Nutr (1992) 56, 77–83.CrossRefGoogle Scholar
Mayer, J & Thomas, DWRegulation of food intake and obesity. Science (1967) 156, 328–337.CrossRefGoogle ScholarPubMed
Meyer, JHThe physiology of gastric motility and gastric emptying. In Textbook of Gastroenterology, vol.1. 137–157 [T, Yamada, DH, Alpers, C, Owyang, DW, Powell and FE, Silverstein, editors]. Philadelphia, PA: JB Lippencott Co (1991)Google Scholar
Meyer, JH, Mayer, EA, Jehn, D, Gu, Y, Fink, AS & Fried, MGastric processing and emptying of fat. Gastroenterology (1986) 90, 1176–1187.CrossRefGoogle ScholarPubMed
Moran, TH & McHugh, PRGastric and nongastric mechanisms for satiety action of cholecystokinin. Am J Physiol (1988) 254, R628–R632.Google ScholarPubMed
Morgan, LMThe metabolic role of GIP: physiology and pathology. Biochem Soc Trans (1996) 24, 585–591.CrossRefGoogle ScholarPubMed
Møller, ALevnedsmiddeltabeller(Food Composition Tables), pp. 28–29, 76–77Søborg, Denmark: Danish Veterinary and Food Administration and Copenhagen, Denmark: Danish Ministry of Health. (1996)Google Scholar
Näslund, E, Grybäck, P, Hellström, PM, Jacobsson, H, Holst, JJ, Theodorsson, E & Backmann, LGastrointestinal hormones and gastric emptying 20 years after jejunoileal bypass for massive obesity. Int J Obes (1997) 21, 387–392.CrossRefGoogle ScholarPubMed
Näslund, E, Bogefors, J, Skogar, S, Grybäck, P, Jacobsson, H, Holst, JJ, Hellström, PMGLP-1 slows solid gastric empytying and inhibits insulin, glucagon, and PYY release in humans. Am J Physiol (1999) 277, R910–R916.Google ScholarPubMed
Nielsen, EWKasein, kaseinmiceller, udfældning af kasein (Casein, caseinmicells and precipitation of casein), pp.1–71. Copenhagen, Denmark: The Laboratorium of Milk, The Royal Veterinary and Agricultural University. (1983)Google Scholar
Ørskov, CReview: glucagon-like-peptide-1, a new hormone of the entero-insular axis. Diabetologia (1992) 35, 701–711.CrossRefGoogle ScholarPubMed
Ørskov, C & Holst, JJRadio-immunoassays for glucagon-like peptides 1 and 2 (GLP-1 and GLP-2). Scand J Clin Lab Invest (1987) 47, 165–174.CrossRefGoogle ScholarPubMed
Patsch, JR, Miesenböck, G, Hopferweiser, T, Mühlberger, V, Knap, E, Dunn, JK, Gotto, AM & Patsch, WRelation of triglyceride metabolism and coronary heart disease. Studies in the postprandial state. Arterioscler Thromb (1992) 12, 1336–1345.CrossRefGoogle ScholarPubMed
Pedersen-Bjergaard, U, Høst, U, Kelbæk, H, Schifter, S, Rehfeld, JF, Faber, J, Christensen, NJInfluence of meal composition on postprandial peripheral plasma concentration of vasoactive peptides in man. Scand J Clin Lab Invest (1996) 56, 497–503.CrossRefGoogle ScholarPubMed
Posati, LP, Kinsella, JF & Watt, BKComprehensive evaluation of fatty acids in foods. J Am Diet Assoc (1975) 66, 482–488.CrossRefGoogle ScholarPubMed
Rehfeld, JFAccurate measurement of cholecystokinin in plasma. Clin Chem (1998) 44, 991–1001.CrossRefGoogle ScholarPubMed
Richelsen, B, Kristensen, K & Pedersen, SBLong-term (6 months) effect of a new fermented milk product on the level of plasma lipoproteins–a placebo-controlled and double blind study. Eur J Clin Nutr (1996) 50, 811–815.Google ScholarPubMed
Rossouw, JE, Burger, E, Van Der Yver, P & Ferreira, JJThe effect of skim milk, yoghurt, and full cream milk on human serum lipids. Am J Clin Nutr (1981) 34, 351–356.CrossRefGoogle ScholarPubMed
Russek, MBrief communication. Demonstration of the influence of an hepatic glucosensitive mechanism on food-intake. Physiol Behav (1970) 5, 1207–1209.CrossRefGoogle Scholar
Sethi, S, Gibney, MJ & Williams, CMPostprandial lipoprotein metabolism. Nutr Res Rev (1993) 6, 161–183.CrossRefGoogle ScholarPubMed
Shimizu, N, Oomura, Y, Novin, D, Grijalva, CV & Cooper, PHFunctional correlations between lateral hypothalamic glucose-sensitive neurons and hepatic portal glucose-sensitive units in rat. Brain Res (1983) 265, 49–54.CrossRefGoogle ScholarPubMed
Siedel, J, Hägele, E, Ziegenhorn, J & Wahlefeld, WReagent for the enzymatic determination of serum total cholesterol with improved lipolytic efficiency. Clin Chem (1983) 29, 1075–1080.CrossRefGoogle ScholarPubMed
Siegel, JA, Urbain, JL, Adler, LP, Charkes, ND, Maurer, AH, Krevsky, B, Knight, LC, Fischer, RS & Malmud, LSBiphasic nature of gastric emptying. Gut (1988) 29, 85–89.CrossRefGoogle ScholarPubMed
Strandhagen, E, Lia Å, , Lindstrand, S, Bergström, P, Lundström, A, Fondén, R & Andersson, HFermented milk (ropy milk) replacing regular milk reduces glycemic responses and gastric emptying in healthy subjects. Scand J Nutr/Näringsforskning (1994) 38, 117–121.Google Scholar
Sykes, S, Morgan, LM, English, J & Marks, VEvidence for preferential stimulation of gastric inhibitory peptide secretion in the rat by actively transported carbohydrates and their analogues. J Endocrinol (1980) 85, 201–207.CrossRefGoogle ScholarPubMed
Taylor, IL & Mannon, PGastrointestinal hormones. In Textbook of Gastroenterology, vol. 1, pp. 24–49 [T, Yamada, DH, Alpers, C, Owyang, DW, Powell and FE, Silverstein, editors]. Philadelphia, PA:: JB Lippencott Co. (1991)Google Scholar
Tholstrup, T, Sandström, BM, Bysted, A & Hölmer, GEffect of 6 dietary fatty acids on the postprandial lipid profile, plasma fatty acids, lipoprotein lipase and cholesterol ester transfer activities in healthy young men. Am J Clin Nutr (2001) 73, 198–208.CrossRefGoogle ScholarPubMed
Thompson, LU, Jenkins, DJA, Vic Amer, MA, Reichert, R, Jenkins, A & Kamulsky, JThe effect of fermented and unfermented milks on serum cholesterol. Am J Clin Nutr (1982) 36 1106–1111CrossRefGoogle ScholarPubMed
Trinder, PDetermination of glucose in blood using glucoseoxidase with an alternative oxygen acceptor. Ann Clin Biochem (1969) 6 24–27CrossRefGoogle Scholar
Urbain, JLC, Siegel, JA, Charkes, D, Maurer, AH, Malmud, LS & Fischer, RSThe two-compartment stomach: effects of particle size on fundal and antral emptying. Eur J Nucl Med (1989) 15, 254–259CrossRefGoogle Scholar
Velchik, MG, Reynolds, JC & Alavi, AThe effect of meal energy content on gastric emptying. J Nucl Med (1989) 30, 1106–1110.Google ScholarPubMed
Wahlefeld, AWTriglycerides determination after enzymatic hydrolyses. In Methods of Enzymatic Analysis, 2nd English ed. 831–1835 [Bergmeyer, HU editor]. New York and London: Verlag Chemie Weinheim and Academic Press (1974)Google Scholar
Walsh, JHGastrointestinal hormones. In Physiology of the Gastrointestinal Tract, 3rd ed. 1–128 [Johnson, LR, Alpers, DH, Christensen, J, Jacobsen, ED and Walsh, JH, editors] New York: Raven Press. (1994)Google Scholar
Wasada, T, McCorkle, K, Harris, V & Kawai, KEffect of gastric inhibitory polypeptide on plasma levels of chylomicron triglycerid in dogs. J Clin Invest (1981) 68 1106–1107CrossRefGoogle ScholarPubMed