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Different effects of casein and soyabean protein on gastric emptying of protein and small intestinal transit after spontaneous feeding of diets in rats

Published online by Cambridge University Press:  09 March 2007

Hiroshi Hara ,
Hiroyuki Nishikawa  and
Shuhachi Kiriyama
Hiroshi Hara
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
Hiroyuki Nishikawa
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
Shuhachi Kiriyama
Affiliation:
Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
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Abstract

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The effects of dietary casein and soyabean-protein isolate (SPI) on gastric emptying and small intestinal transit were observed in rats fed on an 80 g casein or 80 g SPI/kg diet. After a 24 h fast, rats were given 2 g of both the test diets containing 10 g guanidinated casein/kg diet as a marker protein. The amounts of the marker protein remaining in the stomach of the rats fed on the casein or SPI diet were similar and decreased to about 50% after 20min. The emptying rate then slowed, especially in the casein group, so that the amount leaving the stomach after 1 h in the SPI group was slightly higher (P < 0.05). The small intestinal transit of chyme was estimated by a bolus injection of colloidal carbon suspension or of colloidal carbon and 3H-labelled polyethylene glycol through an implanted duodenal catheter 6 min before death. The average value of transit at 12, 20, 40 and 60 min after feeding of SPI diet was about 25% faster than that after casein diet. The transit velocity of the SPI group was also faster than that of the non-protein group 40 min after feeding. These findings reveal that SPI enhances the small intestinal transit of the liquid phase of chyme. There was no correlation between the gastric emptying of homoarginine and small intestinal transit. This result suggests that the small intestinal transit of lumen contents is controlled by the dietary protein regardless of the gastric emptying of protein.

Keywords

Gastric emptyingSmall intestinal transitDietary protein
Type
Diet and Intestinal Function
Information
British Journal of Nutrition , Volume 68 , Issue 1 , July 1992 , pp. 59 - 66
Copyright
Copyright © The Nutrition Society 1992

References

REFERENCES

American Institute of Nutrition (1977). Report of the American Institute of Nutrition ad hoc Committee on Standards for Nutritional Studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Bieri, J. G. (1977). Second report of the ad hoc Committee on Standards for Nutritional Studies. Journal of Nutrition 110, 1726.CrossRefGoogle Scholar
Bidlingmeyer, B. A., Cohen, S. A. & Tarvin, T. L. (1984). Rapid analysis of amino acids using pre-column derivatization. Journal of Chromatography 336, 93–104.CrossRefGoogle ScholarPubMed
Bouyssou, T., Pairet, M., Candau, M. & Ruckebusch, Y. (1988). Effects of intraluminal nutrients on intestinal myoelectric activity in rabbits. American Journal of Physiology 255, G12–G17.Google ScholarPubMed
Bull, J. S., Grundy, D. & Scratcherd, T. (1985). The effect of intraluminal tryptophan and phenylalanine on small intestinal motility in the conscious dog. Journal of Physiology 367, 353–362.CrossRefGoogle ScholarPubMed
Burn-Murdoch, R. A., Fisher, M. A. & Hunt, J. N. (1978). The slowing of gastric emptying by proteins in test meals. Journal of Physiology 274, 477–485.CrossRefGoogle ScholarPubMed
Cohen, S. A., Bidlingmeyer, B. A. & Tarvin, T. L. (1986). PITC derivatives in amino acid analysis. Nature 320, 769–770.CrossRefGoogle ScholarPubMed
Daniel, H., Vohwinkel, M. & Rehner, G. (1990). Effect of casein and β-casomorphins on gastrointestinal motility in rats. Journal of Nutrition 120, 252–257.CrossRefGoogle ScholarPubMed
Debas, H. T., Farooq, O. & Grossman, M. I. (1975). Inhibition of gastric emptying is a physiologic action of cholecystokinin. Gastroenterology 68, 1211–1217.CrossRefGoogle ScholarPubMed
Ebihara, K., Imanura, Y. & Kiriyama, S. (1979). Effect of dietary mineral composition on nutritional equivalency of amino acid mixtures and casein in rats. Journal of Nutrition 109, 2106–2116.CrossRefGoogle ScholarPubMed
Fioramonti, J., Fargeas, M. J. & Bueno, L. (1988). Involvement of endogenous opiates in regulation of gastric emptying of fat test meals in mice. American Journal of Physiology 255, G158–G161.Google ScholarPubMed
Green, G. M., Olds, B. A., Matthews, G. & Lyman, R. L. (1973). Protein, as a regulator of pancreatic enzyme secretion in the rat. Proceedings of the Society for Experimental Biology and Medicine 142, 1162–1167.CrossRefGoogle ScholarPubMed
Habeeb, A. F. S. A. (1960). A new reagent for guanidination of proteins. Canadian Journal of Biochemistry and Physiology 38, 493–501.CrossRefGoogle ScholarPubMed
Hara, H. & Kiriyama, S. (1991). Absorptive behaviors of oligo-l-methionine and dietary proteins in a casein or soybean protein diet: observations by porto-venous difference in unrestrained rats. Journal of Nutrition 121, 638–645.CrossRefGoogle ScholarPubMed
Harper, A. E. (1959). Amino acid balance and imbalance. 1. Dietary level of protein and amino acid imbalance. Journal of Nutrition 68, 405–418.CrossRefGoogle Scholar
Hunt, J. N. & Knox, M. T. (1972). The slowing of gastric emptying by four strong acids and three weak acids. Journal of Physiology 222, 187–208.CrossRefGoogle ScholarPubMed
Kinsman, R. I. & Read, N. W. (1984). Effect of naloxone on feedback regulation of small bowel transit by fat. Gastroenterology 87, 335–337.CrossRefGoogle ScholarPubMed
Konturek, S. J., Radecki, T., Thor, P. & Dembinski, A. (1973). Release of cholecystokinin by amino acids. Proceedings of the Society for Experimental Biology and Medicine 143, 305–309.CrossRefGoogle ScholarPubMed
Kromer, W. (1988). Endogenous and exogenous opioids in the control of gastrointestinal motility and secretion. Pharmacological Reviews 40, 121–162.Google ScholarPubMed
Liddle, R. A., Green, G. M., Conrad, C. K. & Williams, J. A. (1986). Proteins but not amino acids, carbohydrates, or fats stimulate cholecystokinin secretion in the rat. American Journal of Physiology 251, G243–G248.Google ScholarPubMed
Mangel, A. W. & Koegel, A. (1984). Effects of peptides on gastric emptying. American Journal of Physiology 246, G342–G345.Google ScholarPubMed
Moran, T. H. & McHugh, P. R. (1982). Cholecystokinin suppresses food intake by inhibiting gastric emptying. American Journal of Physiology 242, R491–R497.Google ScholarPubMed
Parr, N. J., Grime, S., Critchley, M., Baxter, J. N. & Mackie, C. R. (1988). Mechanisms governing the biphasic pattern of gastric emptying after truncal vagotomy and pyloroplasty. Gut 29, 1253–1257.CrossRefGoogle ScholarPubMed
Poulakos, L. & Kent, T. H. (1973). Gastric emptying and small intestinal propulsion in fed and fasted rats. Gastroenterology 64, 962–967.CrossRefGoogle ScholarPubMed
Read, N. W., Cammack, J., Edwards, C. & Holgate, A. M. (1982). Is the transit time of a meal through the small intestine related to the rate at which it leaves the stomach? Gut 23, 824–828.CrossRefGoogle Scholar
Rogers, Q. R., Chen, M.-L., Peraino, C. & Harper, A. E. (1960). Observations on protein digestion in vivo. III. Recovery of nitrogen from the stomach and small intestine at intervals after feeding diets containing different proteins. Journal of Nutrition 72, 331–339.CrossRefGoogle ScholarPubMed
Schemann, M. & Ehrlein, H.-J. (1986). Postprandial patterns of canine jejunal motility and transit of luminal contents. Gastroenterology 90, 991–1000.CrossRefGoogle Scholar
Spiller, R. C., Trotman, I. F., Adrian, T. E., Bloom, S. R., Misiewicz, J. J. & Silk, D. B. A. (1988). Further characterisation of the ‘ileal brake’ reflex in man – effect of ileal infusion of partial digests of fat, protein, and starch on jejunal motility and release of neurotensin, enteroglucagon, and peptide YY. Gut 29, 1042–1051.CrossRefGoogle ScholarPubMed
Summers, R. W., Kent, T. H., & Osborne, J. W. (1970). Effects of drugs, ileal obstruction, and irradiation on rat gastrointestinal propulsion. Gastroenterology 59, 731–739.CrossRefGoogle ScholarPubMed