Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-23T07:31:01.826Z Has data issue: false hasContentIssue false
  • English
  • Français

Difference in the metabolism of vitamin K between liver and bone in vitamin K-deficient rats

Published online by Cambridge University Press:  09 March 2007

Toshiro Sato ,
Yutaka Ohtani ,
Yoko Yamada ,
Sanshiroh Saitoh  and
Hiroshi Harada
Toshiro Sato*
Affiliation:
Chemicals Research Laboratories, Honen Corporation, Asaba-cho, Iwata-gun, Shizuoka 437-1111, Japan
Yutaka Ohtani
Affiliation:
Chemicals Research Laboratories, Honen Corporation, Asaba-cho, Iwata-gun, Shizuoka 437-1111, Japan
Yoko Yamada
Affiliation:
Chemicals Research Laboratories, Honen Corporation, Asaba-cho, Iwata-gun, Shizuoka 437-1111, Japan
Sanshiroh Saitoh
Affiliation:
Food Research Laboratories, Honen Corporation, Shimizu City, Shizuoka 424-0824, Japan
Hiroshi Harada
Affiliation:
Department of Nutritional Science, Faculty of Applied Biological Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
*
*Corresponding author: Dr T. Sato, fax +81 538 23 6772, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The difference between vitamin K metabolism in the liver and that in the bone of vitamin K-deficient rats was examined. After 17 d administration of vitamin K-deficient food, vitamin K in the liver was almost depleted, and prothrombin time (PT) was prolonged. Serum total osteocalcin level was slightly decreased by vitamin K deficiency, whereas serum undercarboxylated osteocalcin level did not change. The level of menaquinone (MK)-4 as well as that of phylloquinone was decreased, but approximately 40 % of the initial level still existed in the femur after the 17 d period. A single-dose administration of vitamin K (250 nmol/kg body weight) markedly increased vitamin K level in the liver but not in the femur. These results suggest that the turnover of vitamin K in the bone is slower than that in the liver, and bone metabolism may be little affected by the short period of intake of vitamin K-deficient food. However, intake of a larger amount of vitamin K is required for its accumulation in the bone than in the liver. Furthermore, the counteracting effect of MK-7 on prolonged PT in vitamin K-deficient rats was found to be higher than phylloquinone or MK-4.

Keywords

MenaquinonePhylloquinoneVitamin KCoagulationOsteocalcin
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 4 , April 2002 , pp. 307 - 314
Copyright
Copyright © The Nutrition Society 2002

References

Akiyama, Y, Hara, K, Matsumoto, A, Takahara, S & Tajima, T (1995) Comparison of intestinal absorption of vitamin K2 (menaquinone) homologues and their effects on blood coagulation in rats with hypoprothrombinaemia. Biochemical Pharmacology 49, 1801–1807.CrossRefGoogle ScholarPubMed
Barns, RH & Fiala, G (1959) Effects of prevention of coprophagy in the rat. VI. Vitamin K1. Journal of Nutrition 68, 603–614.CrossRefGoogle Scholar
Booth, SL & Suttie, JW (1998) Dietary intake and adequacy of vitamin K. Journal of Nutrition 128, 785–788.CrossRefGoogle ScholarPubMed
Collins, MD & Jones, D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiological Review 45, 316–354.CrossRefGoogle Scholar
Conly, JM & Stein, K (1992) Quantitative and qualitative measurements of K vitamins in human intestinal contents. American Journal of Gastroenterology 87, 311–316.Google ScholarPubMed
Craciun, AM, Groenen-van Dooren, MMCL, Thijssen, HHW & Vermeer, C (1998) Induction of prothrombin synthesis by K-vitamins compared in vitamin K-deficient and in brodifacoum-treated rats. Biochimica et Biophysica Acta 1380, 75–81.CrossRefGoogle ScholarPubMed
Davidson, RT, Foley, AL, Engelle, JA & Suttie, JW (1998) Conversion of dietary phylloquinone to tissue menaquinone-4 in rats is not dependent on gut bacteria. Journal of Nutrition 128, 220–223.CrossRefGoogle Scholar
Groenen-van Dooren, MMCL, Ronden, JE, Soute, BAM & Vermeer, C (1993) The relative effects of phylloquinone and menaquinone on the blood coagulation factor synthesis in vitamin K-deficient rats. Biochemical Pharmacology 50, 797–801.CrossRefGoogle Scholar
Groenen-van Dooren, MMCL, Ronden, JE, Soute, BAM & Vermeer, C (1995) Bioavailability of phylloquinone and menaquinones after oral and colorectal administration in vitamin K-deficient rats. Biochemical Pharmacology 50, 797–801.CrossRefGoogle ScholarPubMed
Haffa, A, Krueger, D, Bruner, J, Engelke, J, Gundberg, C, Akhter, M & Binkley, N (2000) Diet- or warfarin-induced vitamin K insufficiency elevates circulating undercarboxylated osteocalcin without altering skeletal status in growing female rats. Journal of Bone and Mineral Research 15, 872–878.CrossRefGoogle ScholarPubMed
Hara, K, Akiyama, Y, Nakamura, T, Murata, S & Morita, I (1995) The inhibitory effect of vitamin K2 (menatetrenone) on bone resorption may be related to its side chain. Bone 16, 179–184.CrossRefGoogle Scholar
Hirauchi, K, Sakano, T, Notsumoto, S, Nagaoka, T, Morimoto, A, Fujimoto, K, Masuda, S & Suzuki, Y (1989) Measurement of K vitamins in foods by high-performance liquid chromatography with fluorometric detection. Vitamins (Japan) 63, 147–151.Google Scholar
Hodges, SJ, Akesson, K, Vergnaud, P, Obrant, K & Delmas, PD (1993 a) Circulating levels of vitamins K1 and K2 decreased in elderly women with hip fracture. Journal Bone and Mineral Research 8, 1241–1245.CrossRefGoogle ScholarPubMed
Hodges, SJ, Bejui, J, Leclercq, M & Delmas, PD (1993 b) Detection and measurement of vitamins K1 and K2 in human cortical and trabecular bone. Journal of Bone and Mineral Research 8, 1005–1008.CrossRefGoogle ScholarPubMed
Kaneki, M, Mizuno, Y, Hosoi, T, Inoue, S, Hoshino, S, Akishita, M, Akedo, Y, Horiki, K, Ouchi, H & Orimo, H (1995) Taikouki-kotsusosyousyou niokeru kessei vitamin K noudo no kentou (Serum concentration of vitamin K in elderly women with involutional osteoporosis). Nihon Ronen Igaku Zasshi 32, 195–200.CrossRefGoogle Scholar
Koshihara, Y, Hoshi, K, Ishibashi, H & Shiraki, M (1996) Vitamin K2 promotes 1α,25(OH)2 vitamin D3-induced mineralization in human periosteal osteoblasts. Calcified Tissue International 59, 466–473.Google Scholar
Koshihara, Y, Hoshi, K & Shiraki, M (1992) Enhancement of mineralization of human osteoblasts by vitamin K2 (menatetrenone). Journal of Clinical and Experimental Medicine 161, 439–440.Google Scholar
Mathers, JC, Fernandes, F, Hill, MJ, McCarthy, PT, Shearer, MJ & Oxley, A (1990) Dietary modification of potential vitamin K supply from enteric bacterial menaquinones in rats. British Journal of Nutrition 63, 639–652.CrossRefGoogle ScholarPubMed
Merle, B & Delmas, PD (1990) Normal carboxylation of circulating osteocalcin (bone Gla protein) in Paget's disease of bone. Bone and Mineral 11, 237–245.CrossRefGoogle ScholarPubMed
Nakanishi, T, Furukawa, M, Okuda, H, Iizuka, B & Obata, H (1989) Comparison of effects of menaquinone-4 and menaquinone-7 assessed by decrease of PIVKA-II levels in human hepatoma cells in culture. Vitamins (Japan) 63, 253–257.Google Scholar
Poser, JW & Price, PA (1978) A method for decarboxylation of γ-carboxyglutamic acid in proteins. Journal of Biological Chemistry 254, 431–436.CrossRefGoogle Scholar
Price, PA (1985) Vitamin K-dependent formation of bone gla protein (osteocalcin) and its function. Vitamins and Hormones 42, 65–108.CrossRefGoogle ScholarPubMed
Ronden, JE, Drittij-Reijnders, MJ, Vermeer, C & Thijssen, HH (1998) Intestinal flora is not an intermediate in the phylloquinone–menaquinone-4 conversion in the rat. Biochimica et Biophysica Acta 1379, 69–75.CrossRefGoogle Scholar
Sakano, T, Notsumoto, S, Nagaoka, T, Morimoto, A, Fujimoto, K, Masuda, S, Suzuki, Y & Hirauchi, K (1998) Measurement of K vitamins in food by high performance liquid chromatography with fluorometric detection. Vitamins (Japan) 62, 393–398.Google Scholar
Sato, T, Yamada, Y, Ohtani, Y, Mitsui, N, Murasawa, H & Araki, S (2001) Production of menaquinone (vitamin K2)-7 by Bacillus subtilis. Journal of Bioscience and Bioengineering 91, 16–21.CrossRefGoogle ScholarPubMed
Shearer, MJ (1990) Vitamin K and vitamin K-dependent proteins. British Journal of Haematology 75, 156–162.Google ScholarPubMed
Shearer, MJ, Bach, A & Kohlemeier, M (1996) Chemistry, nutritional source, tissue distribution and metabolism of vitamin K with special reference to bone health. Journal of Nutrition 126(Suppl.), 1181S–1186S.CrossRefGoogle ScholarPubMed
Shiraki, M, Shiraki, Y, Aoki, C & Miura, M (2000) Vitamin K2 (menatetrenone) effectively prevents fractures and sustains lumbar bone mineral density in osteoporosis. Journal of Bone and Mineral Research 15, 515–521.CrossRefGoogle ScholarPubMed
Sokoll, JJ, Booth, SL, O'Brien, ME, Davidson, KW, Tsaioun, KI & Sadowski, JA (1997) Changes in serum osteocalcin, plasma phylloquinone, urinary γ-carboxyglutamic acid in response to altered intakes of dietary phylloquinone in human subjects. American Journal of Clinical Nutrition 65, 779–784.CrossRefGoogle ScholarPubMed
Suttie, JW (1995) The importance of menaquinones in human nutrition. Annual Reviews of Nutrition 15, 399–417.CrossRefGoogle ScholarPubMed
Szulc, P, Arlot, M, Chapuy, M-C, Duboeuf, F, Meunier, PJ & Desmas, PD (1994) Serum undercarboxylated osteocalcin correlates with hip bone mineral density in elderly women. Journal of Bone and Mineral Research 9, 1591–1595.CrossRefGoogle ScholarPubMed
Szulc, P, Chapuy, M-C, Meunier, PJ & Delmas, PD (1993) Serum undercarboxylated osteocalcin is a marker of the hip fracture in elderly women. Journal of Clinical Investigation 91, 1769–1774.CrossRefGoogle Scholar
Thijssen, HHW & Drittij-Reijnders, MJ (1994) Vitamin K distribution in rat tissues: dietary phylloquinone is a source of tissue menaquinone-4. British Journal of Nutrition 72, 415–425.CrossRefGoogle ScholarPubMed
Tsukamoto, Y, Ichise, H, Kakuda, H & Yamaguchi, M (2000) Intake of fermented soybean (natto) increases circulating vitamin K2 (menaquinone-7) and γ-carboxylated osteocalcin concentration in normal individuals. Journal of Bone and Mineral Metabolism 18, 216–222.CrossRefGoogle ScholarPubMed
Uchida, K, Nomura, Y, Takase, H, Harauchi, T, Yoshizaki, T & Nakao, H (1986) Effects of vitamin K-deficient diets and fasting on blood coagulation factors in conventional and germfree rats. Japan Journal of Pharmacology 40, 115–122.CrossRefGoogle Scholar
Vermeer, C, Jie, K-SG & Knapen, MHJ (1995) Role of vitamin K in bone metabolism. Annual Reviews of Nutrition 15, 1–22.CrossRefGoogle ScholarPubMed
Yamaguchi, M, Taguchi, H, Gao, YH, Igasshi, A & Tsukamoto, Y (1999) Effect of vitamin K2 (menaquinone-7) in fermented soybean (natto) on bone loss in ovariectomized rats. Journal of Bone and Mineral Metabolism 17, 23–29.CrossRefGoogle Scholar
Yamamoto, R, Komai, M, Kojima, K, Furukawa, Y & Kimura, S (1997) Menaquinone-4 accumulation in various tissues after an oral administration of phylloquinone in Wistar rats. Journal of Nutritional Science and Vitaminology (Tokyo) 42, 133–143.CrossRefGoogle Scholar