Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-22T23:38:36.409Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary sesame (Sesamum indicum cultivar Linn) oil inhibits iron-induced oxidative stress in rats

Published online by Cambridge University Press:  09 March 2007

S. Hemalatha ,
M. Raghunath  and
Ghafoorunissa
S. Hemalatha
Affiliation:
National Institute of Nutrition, Indian Council of Medical Research, Jamai Osmania P. O., Hyderabad-500 007. A. P., India
M. Raghunath
Affiliation:
National Institute of Nutrition, Indian Council of Medical Research, Jamai Osmania P. O., Hyderabad-500 007. A. P., India
Ghafoorunissa*
Affiliation:
National Institute of Nutrition, Indian Council of Medical Research, Jamai Osmania P. O., Hyderabad-500 007. A. P., India
*
*Corresponding author: Dr Ghafoorunissa, fax +91 40 27019074, email [email protected] and [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The high stability of sesame oil against oxidative deterioration is attributed to lignans in its non-glycerol fraction. The present study evaluates the effects of feeding sesame lignans (sesamin and sesamolin) on Fe2+-induced oxidative stress in rats. Three groups, each of sixteen male weanling WNIN rats, were fed diets containing 200 g casein/kg and 100 g oil/kg (group 1, groundnut oil; group 2, sesame oil; group 3, sesame oil+sesamin (0·4 g/kg). After 45 d of feeding, eight rats from each group were injected with saline (9 g Na Cl/l, controls) intraperitoneally while the remaining eight rats were injected with 30 mg Fe2+/kg body weight as ferrous sulfate in normal saline. The animals were killed after 90 min to evaluate hepatic function and antioxidant status. Compared with those fed groundnut oil (group 1), sesame oil-fed rats (groups 2 and 3) had lower levels of hepatic thiobarbituric acid-reactive substances, serum glutamate:oxaloacetate transaminase activities and serum glutamate pyruvate transaminase activities, indicating protection against Fe-induced oxidative stress. Despite similar tocopherol levels in the three diets, hepatic α-tocopherol levels were higher in rats fed the sesame-oil diets (groups 2 and 3) compared with controls (group 1). However, activities of hepatic antioxidant enzymes (superoxide dismutase and glutathione peroxidase) were significantly (P<0·05) increased only in rats fed higher levels of lignans (group 3). These observations suggest that sesame lignans may have sparing effects on tocopherols. The increased bioavailability of tocopherols in the presence of dietary lignans might be due to the regeneration of oxidized tocopherols. The synergistic effects of lignans with tocols has nutritional and therapeutic implications.

Keywords

Sesame lignansSesaminSesamolinIron-induced oxidative stress in vivoAntioxidant effects
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 4 , October 2004 , pp. 581 - 587
Copyright
Copyright © The Nutrition Society 2004

References

Aebi, HE (1983) Catalase. In Methods in Enzymatic Analysis, vol III, pp. 273–282 [Bergmeyer, HU, editors] Weinhein: Verlog ChemieGoogle Scholar
Akimoto, K, Kitagawa, Y, Akamatsu, T, Hirose, N, Sugano, M, Shimizu, S & Yamada, H (1993) Protective effects of sesamin caused by alcohol or carbon tetra chloride in rodents Ann Nutr Met 37, 218–224CrossRefGoogle Scholar
Asami, S, Akimoto, K, Abe, K, Akamatsu, T, Konishi, K, Shimizu, S, Sugano, M & Yamada, H (1993) Antioxidant activity of sesamin on NADPH dependent lipid peroxidation in liver microsomes.Nippon Nogeikagaku Kaishi 67, 265Google Scholar
Barrie, T & Linda, B (1989) Separation of tocopherol and tocotrienol isomers using normal and reverse phase liquid chromatography. Anal Biochem 180, 368–373Google Scholar
Bhattacharya, A, Ramanathan, M, Ghosal, S & Bhattacharya, SK (2000) Effect of Withania somnifera glycowithanolides on iron induced hepatotoxicity in rats. Phytother Res 14, 568–5703.0.CO;2-Q>CrossRefGoogle Scholar
Bligh, EG & Dyer, WJ (1959) A rapid method of total lipid extraction of lipids in tissues. Can J Biochem Physiol 37, 911–917CrossRefGoogle Scholar
Budowski, P (1950) Sesame oil. 111. Antioxidant properties of sesamol. J Am Oil Chem Soc 27, 264–267CrossRefGoogle Scholar
Budowski, P & Markley, KS (1951) The chemical and physiological properties of sesame oil. Chem Rev 48, 125–151CrossRefGoogle ScholarPubMed
Fukuda, Y, Nagata, M & Namiki, M (1986 a) Chemical aspects of the antioxidative activity of roasted sesame seed oil and the effect of using the oil for frying. Agric Biol Chem 50, 857–862Google Scholar
Fukuda, Y, Nagata, M, Osawa, T & Namiki, M (1986 b) Contribution of lignan analogues to antioxidant activity of refined unroasted sesame seed oil. J Am Oil Chem Soc 63, 1027–1030CrossRefGoogle Scholar
Fujiwara, FY, Sawada, UR, Kuzuyama, M & Igarashi, O (1995) Effects of sesamin on fatty acid composition of liver of rats fed n -6 and n -3 fatty acid rich diet. J Nutr Sci Vitaminol 41, 217–225CrossRefGoogle Scholar
Gao, ZL & Rao, EC (1993) Synthesis of formyl (acetyl) amino acid and dipeptide derivatives of amino ketones. Yao Xue Xue Bao 28, 744–757Google Scholar
Ghafoorunissa, , Hemalatha, S, Vishnu Vardhana Rao, M (2004) Sesame lignans enhance the antioxidant activity of vitamin E in lipid peroxidation systems. Mol Cell Biochem In the PressCrossRefGoogle Scholar
Gu, JL, Wakizono, Y, Tsujita, A, Lim, B, Nonaka, M, Yamada, K & Sugano, M (1995) Effects of sesamin and alpha tocopherol individually or in combination on the polyunsaturated fatty acid metabolism chemical mediator production and immuno globulin levels in Sprague-Dawley rats. Biosci Biotech Biochem 59, 2198–2202CrossRefGoogle Scholar
Gu, JY, Tsujitha, A, Wakizono, Y, Yamada, K & Sugano, M (1997) Combined effects of sesamin with alpha tocopherol or tocotrienols on lipid and immune indices in Brown Norway rats. Nutr Res 17, 339–350CrossRefGoogle Scholar
Gu, JY, Wakijano, Y, Dohi, A, Nonaka, M, Sugano, M & Yamada, K (1998) Effects of dietary fats and sesamin on lipid metabolism and immune function of Sprague-Dawley rats. Biosci Biotech Biochem 62, 1917–1924CrossRefGoogle Scholar
Hemalatha, S & Ghafoorunissa, (2004) Lignans and tocopherols in Indian sesame cultivars. J Am Oil Chem Soc In the PressCrossRefGoogle Scholar
Hirata, F, Fujita, K, Ishikura, Y, Hosada, K, Ishikawa, T & Nakamura, H (1996) Hypocholesterolemic effect of sesame lignan in humans. Atherosclerosis 122, 135–136CrossRefGoogle Scholar
Hirose, N, Doi, F, Ueki, T, Akazawa, K, Chijiiwa, K, Sugano, M, Akimoto, K, Shimizu, S & Yamada, K (1992) Suppressive effect of sesamin against 7,12-dimethyl benzanthracene induced rat mammary carcinogenesis. Anticancer Res 12, 1259–1266Google Scholar
Hou, RCW, Huang, HM, Tzen, JTC & Jeng, KCG (2003) Protective effects of sesamin and sesamolin on hypoxic neuronal PC12 cells. J Neurosci Res 74, 123–133CrossRefGoogle Scholar
Hu, ML, Edwin, NF & Tappel, AL (1990) Effect of dietary menhaden oil and vitamin E on the in vivo lipid peroxidation induced by iron. Lipids 25, 194–198CrossRefGoogle Scholar
Kamal-Eldin, A, Patterson, D & Appelqvist, LA (1995) Sesamin (a compound from sesame oil) increases tocopherol levels in rats fed ad libitum. Lipids 10, 499–505CrossRefGoogle Scholar
Kang, M, Oaito, M, Tsujihara, N & Osawa, T (1998) Sesamolin inhibits lipid peroxidation in rat liver and kidney. J Nutr 128, 1018–1022CrossRefGoogle Scholar
Lueng, HW, Vang, MJ & Maris, RD (1982) The cooperative interaction between vitamin E and vitamin C in suppression of peroxidation of membrane phospholipids. Biochem Biophys Acta 264, 266–272Google Scholar
Macrae, WD & Towers, NGH (1984) Biological activities of lignans. Phytochemistry 23, 1207–1220CrossRefGoogle Scholar
Marklund, S & Marklund, G (1974) Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47, 469–474CrossRefGoogle Scholar
Matsumura, Y, Kita, S, Morimoto, S, Akimoto, K, Furuya, M, Oka, N & Tanaka, T (1995) Antihypertensive effect of sesamin. i. Protection against deoxycorticosterone acetate-salt-induced hypertension and cardiovascular hypertrophy. Biol Pharm Bull 18, 1016–1019CrossRefGoogle Scholar
Matsumura, Y, Kita, S, Tanida, Y, Taguchi, Y, Morimoto, S, Akimoto, K & Tanaka, T (1998) Antihypertensive effect of sesamin. iii. Protection against development and maintenance of hypertension in stroke prone spontaneously hypertensive rats. Biol Pharm Bull 21, 469–473CrossRefGoogle Scholar
Meydani, M (1992) Protective role of dietary vitamin E on oxidative stress in aging. Age 15, 89–93CrossRefGoogle Scholar
Miyahara, Y, Hibasami, H, Katsuzaki, H, Imai, K & Komiya, T (2001) Sesamolin from sesame seed inhibits proliferation by inducing apoptosis in human lymphoid leukemia molt 4B cells. Int J Mol Med 7, 369–371Google Scholar
Nakabayashi, A, Kitagawa, Y, Suwa, Y, Akimoto, K, Asami, S, Shimizu, S, Hirose, N, Sugano, M & Yamada, H (1995) α-Tocopherol enhances the hypocholesterolemic action of sesamin in rats. Int J Vit Nutr Res 65, 162–168Google ScholarPubMed
Niederau, C, Fischer, R, Sonnenberg, A, Streemeel, W, Trampish, HJ & Strotimeye, G (1985) Survival and causes of death in cirrhotic and in non cirrhotic patients with primary hemochromatosis. N Engl J Med 313, 1256–1262CrossRefGoogle Scholar
Paglia, DE & Valentine, WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70, 158–169Google Scholar
Parker, SP, Timothy, JS & Joy, ES (2000) Cytochrome P450 3A-dependent metabolism of tocopherols and inhibition by sesamin. Biochem Biophys Res Commun 277, 531–534CrossRefGoogle Scholar
Press, M, Hartop, PJ & Proney, C (1974) Correction of essential fatty acid deficiency in man by cutaneous application of sunflower seed oil. Lancet 1, 597–598CrossRefGoogle Scholar
Pulla Reddy, ACH & Lokesh, BR (1994) Effect of dietary tumeric ( Curcuma longa ) on iron-induced lipid peroxidation in the rat liver. Food Chem Toxicol 32, 279–283CrossRefGoogle Scholar
Reeves, PG, Nielsen, FH & Fahey, GC (1993) AIN-93 purified diets for laboratory rodents. J Nutr 123, 1939–1951CrossRefGoogle Scholar
Satchithanandam, S, Chanderbhan, R, Kharroubi, AT, Calvert, RJ, Klufold, D, Tepper, SA & Kritchevsky, D (1996) Effect of sesame oil on serum and liver lipid profile in the rat. Int J Vit Nutr Res 66, 386–392Google Scholar
Schacterle, GR & Pollack, RL (1973) A simplified method for the quantative assay of small amounts of protein in biologic material. Anal Biochem 51, 654–655CrossRefGoogle Scholar
Scholz, KS, Liken, AD, Gumpricht, E & Reddy, CD (1997) Glutathione dependent factors and inhibition of rat liver microsomal lipid peroxidation. Free Radic Biol Med 23, 815–828CrossRefGoogle Scholar
Slater, TF & Sawyer, BC (1971) The stimulatory effects of carbon tetrachloride and other halogeno-alkanes on peroxidative reactions in rat liver fractions in vitro. Biochem J 123, 805–814CrossRefGoogle Scholar
Sugano, M, Inoule, T, Koba, K, Yoshida, K, Hirose, N, Shinmen, Y, Akimoto, K & Amachi, T (1990) Influence of sesame lignans on various lipid parameters in rats. Agric Biol Chem 54, 2669–2673Google Scholar
Sugano, M & Akimoto, K (1993) Sesamin: a multifunctional gift from nature. J Chin Nutr Sci 18, 1–11Google Scholar
Uchida, M, Nakajin, S, Toyoshima, S & Shinoda, M (1996) Antioxidant effect of sesamol and related compounds on lipid peroxidation. Biol Pharm Bull 19, 623–626CrossRefGoogle ScholarPubMed
Yamashita, K, Ikeda, S, Lizuka, Y & Ikeda, Y (2002) Effect of sesaminol on plasma and tissue α-tocopherol and α tocotrienol concentrations in rats fed a vitamin E concentrate rich in tocotrienols. Lipids 37, 351–358CrossRefGoogle Scholar
Yamashita, K, Kagaya, M, Higuti, N & Kiso, Y (2000) Sesamin and α-tocopherol synergistically suppress lipid peroxide in rats fed a high docosahexaenoic acid diet. Biofactors 11, 11–13CrossRefGoogle Scholar
Yamashita, K, Lizuka, Y, Imai, T & Namiki, M (1995) Sesame seed and its lignans produce marked enhancement of vitamin E activity in rats fed a low tocopherol diet. Lipids 30, 1019–1028CrossRefGoogle Scholar
Yamashita, K, Nohara, Y, Katayama, K & Namiki, M (1992) Sesame seed lignans and γ-tocopherol act synergistically to produce vitamin E activity in rats. J Nutr 122, 2440–2446CrossRefGoogle Scholar
Zhu, QY, Huang, YY & Chen, ZY (2000) Interaction between flavonoids and a tocopherol in human low density lipoprotein. J Nutr Biochem 11, 14–21CrossRefGoogle Scholar