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Dietary rhubarb (Rheum rhaponticum) stalk fibre does not lower plasma cholesterol levels in diabetic rats

Published online by Cambridge University Press:  09 March 2007

Sukhinder Kaur Cheema ,
Vinti Goel ,
Tapan K. Basu  and
Luis B. Agellon
Sukhinder Kaur Cheema*
Affiliation:
Department of Biochemistry, University of Alberta, Edmonton, Alberta, CanadaT6G 2S2 Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, CanadaA1B 3X9
Vinti Goel
Affiliation:
Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, Alberta, CanadaT6G 2P5
Tapan K. Basu
Affiliation:
Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, Alberta, CanadaT6G 2P5
Luis B. Agellon
Affiliation:
Department of Biochemistry, University of Alberta, Edmonton, Alberta, CanadaT6G 2S2
*
*Corresponding author: Dr Sukhinder Kaur Cheema, fax +1 709 737 2422, email [email protected]
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Abstract

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Rhubarb (Rheum rhapontiam) stalk fibre was previously shown to be hypolipidaemic under clinical and experimental conditions. The present study was undertaken to investigate whether rhubarb stalk fibre has a hypolipidaemic effect under diabetic conditions. Two models of diabetic rats were used: streptozotocin-induced diabetic rats, and diabetes-prone BB (BBdp) rats. The plasma cholesterol and triacylglycerol concentrations were elevated after the onset of diabetes in BBdp rats, but not in sterptozotocin-induced diabetic rats. The rhubarb-fibre diet had no effect on the plasma cholesterol or triacylglycerol concentrations of diabetic rats. The hypolipidaemic effect of rhubarb stalk fibre has been suggested to be due to the bile-acid-binding capacity of rhubarb fibre, which in turn up regulates cholesterol 7α-hydroxylase (cyp7a) activity. cyp7a is the first and the rate-limiting enzyme in the breakdown of cholesterol to bile acids. We measured the cyp7a activity and mRNA levels in control and diabetic rats fed rhubarb- and cellulose-fibre diets. The cyp7a activity and mRNA abundance were increased in both diabetic rat models, indicating that bile acid synthesis is enhanced in diabetes. Feeding a diet enriched with rhubarb fibre caused a slight but significant increase (P<0·05) in cyp7a enzyme activity in BBdp rats, but no change in cyp7a mRNA abundance was detected. These results suggest that although a rhubarb-fibre-enriched diet increased cyp7a activity in BBdp rats, there was no apparent therapeutic benefit in terms of lowering plasma cholesterol concentrations.

Keywords

DiabetesRhubarb (Rheum rhapontioum) stalk fibreCholesterol 7α-hydroxylase
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 2 , February 2003 , pp. 201 - 206
Copyright
Copyright © The Nutrition Society 2003

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