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Dietary conjugated linoleic acid mixture affects the activity of intestinal acyl coenzyme A: cholesterol acyltransferase in hamsters

Published online by Cambridge University Press:  09 March 2007

Chi Hang Thomas Yeung ,
Lin Yang ,
Yu Huang ,
Jun Wang  and
Zhen-Yu Chen
Chi Hang Thomas Yeung
Affiliation:
Department of Biochemistry and, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
Lin Yang
Affiliation:
Department of Biochemistry and, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Department of Chemistry, Henan Normal University, Xinxiang, Heanan, The People's Republic of China
Yu Huang
Affiliation:
Physiology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
Jun Wang
Affiliation:
Department of Biochemistry and, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
Zhen-Yu Chen*
Affiliation:
Department of Biochemistry and, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong
*
*Corresponding author: Dr Zhen-Yu Chen, fax +852 2603 5123 email [email protected]
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Abstract

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The present study was designed to study the mechanisms by which dietary conjugated linoleic acids (CLA) decrease serum cholesterol. Hamsters were fed a semi-synthetic diet containing 1 g cholesterol/kg diet with or without supplementation with 20 g linoleic acid (LA) and 20 g CLA/kg diet. After 8 weeks, serum fasting total cholesterol (TC) and triacylglycerol (TG) were significantly lower in the LA-supplemented and CLA-supplemented groups compared with those of the control (CTL) hamsters. In contrast to LA, CLA significantly lowered hepatic cholesterol but it increased the level of adipose tissue cholesterol, suggesting that the hypocholesterolaemic mechanism of CLA is different from that of LA. CLA decreased the activity of intestinal acyl CoA:cholesterol acyltransferase (ACAT) whereas LA had no effect on this enzyme. Consequently, CLA supplementation increased the faecal excretion of total neutral sterols, but it had no or little effect on the faecal acidic sterols. If the ACAT is associated with cholesterol absorption, the part of mechanisms by which CLA decreases serum cholesterol may involve down-regulation of intestinal ACAT activity.

Keywords

CholesterolConjugated linoleic acidsLinoleic acidTriacylglycerol
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 6 , December 2000 , pp. 935 - 941
Copyright
Copyright © The Nutrition Society 2000

References

Belury, MA & Kempa-Steczko, A (1997) Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 32, 199–204.CrossRefGoogle ScholarPubMed
Cawood, P, Wichens, DG, Iversen, SA, Braganza, JM & Dormandy, TL (1983) The nature of diene conjugation in human serum, bile and duodenal juice. FEBS Letters 162, 239–243.CrossRefGoogle ScholarPubMed
Chan, PT, Fong, WP, Cheung, YL, Huang, Y, Ho, WKK & Chen, ZY (1999) Jasmine green tea epicatechins are hypolipidemic in hamsters (Mesocricetus auratus) fed a high-fat diet. Journal of Nutrition 129, 1094–1101.CrossRefGoogle Scholar
Chen, ZY, Chan, PT, Kwan, KY & Zhang, A (1997) Reassessment of the antioxidant activity of conjugated linoleic acids. Journal of American Oil Chemists' Society 74, 749–753.CrossRefGoogle Scholar
Chin, SF, Liu, W, Storkson, JM, Ha, YL & Pariza, MW (1992) Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. Journal of Food Composition and Analysis 5, 185–197.CrossRefGoogle Scholar
de Deckere, EAM, van Amelsvoort, JMM, McNeill GP and Jones, P (1999) Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster. British Journal of Nutrition 82, 309–317.CrossRefGoogle ScholarPubMed
DeLany, JP, Blohm, F, Trutt, AA, Scimeca, JA & West, DB (1999) Conjugated linoleic acid rapidly reduces body fat content in mice without affecting energy intake. American Journal of Physiology 276, R1172–R1179.Google ScholarPubMed
Erickson, SK, Cooper, AD, Matsui, SM & Gould, RG (1977) 7-Ketocholesterol: its effects on hepatic cholesterogenesis and its hepatic metabolism in vivo and. in vitro. Journal of Biological Chemistry 252, 5186–5193.CrossRefGoogle ScholarPubMed
Grundy, SM (1986) Cholesterol and coronary heart disease: A new era. Journal of American Medical Association 256, 2849–2858.CrossRefGoogle ScholarPubMed
Grundy, SM & Denke, MA (1990) Dietary influences on serum lipids and lipoproteins. Journal of Lipid Research 31, 1149–1172.CrossRefGoogle ScholarPubMed
Ha, YL, Grimm, NK & Pariza, MW (1989) Newly recognized anticarcinogenic fatty acids: identification and quantification in natural and processed cheeses. Journal of Agricultural and Food Chemistry 37, 75–81.CrossRefGoogle Scholar
Heider, JG, Pickens, GE & Kelly, LA (1983) Role of acyl CoA: cholesterol acyltransferase in cholesterol absorption and its inhibition by 57-118 in the rabbit. Journal of Lipid Research 24, 1127–1134.CrossRefGoogle ScholarPubMed
Helgerud, P, Saarem, K & Norum, KR (1981) Acyl-CoA: cholesterol acyltransferase in human small intestine: its activity and some properties of the enzymic reaction. Journal of Lipid Research 22, 271–277.CrossRefGoogle ScholarPubMed
Heller, RA & Strewsbury, MA (1976) 3-Hydroxy-3-methylglutaryl coenzyme A reductase from rat liver. Its purification, properties and immunochemical study. Journal of Biological Chemistry 251, 3815–3822.CrossRefGoogle Scholar
Hughes, PE, Hunter, WJ & Tove, SB (1982) Biohydrogenation of unsaturated fatty acids: purification and properties of cis-9, trans-11 octodecadienoic acid reductase. Journal of Biological Chemistry 257, 3643–3649.CrossRefGoogle Scholar
Iversen, SA, Cawood, P, Madigan, MJ, Lawson, AM & Dormandy, TL (1985) Identification of a diene conjugated component of human lipid as octadeca-9,11-dienoic acid. FEBS Letters 171, 320–324.CrossRefGoogle Scholar
Kellog, TF (1974) Steroid balance and tissue cholesterol accumulation in germfree and conventional rats fed diets containing saturated and polyunsaturated fats. Journal of Lipid Research 15, 574–579.CrossRefGoogle Scholar
Kepler, CR & Tove, SB (1967) Biohydrogenation of unsaturated fatty acids. Journal of Biological Chemistry 242, 5606–5612.CrossRefGoogle ScholarPubMed
Largis, EE, Wang, CH, De Vries, VG & Schaffer, SA (1989) CL 277, 082: a novel inhibitor of ACAT-catalyzed cholesterol esterification and cholesterol absorption. Journal of Lipid Research 30, 681–690.CrossRefGoogle ScholarPubMed
Lee, KN, Kritchevsky, D & Pariza, MW (1994) Conjugated linoleic acids and atherosclerosis in rabbits. Atherosclerosis 108, 19–25.CrossRefGoogle ScholarPubMed
Lehmann, R, Bhargava, AS & Günzel, P (1993) Serum lipoprotein in rats, dogs and monkeys, including method comparison and influence of menstrual cycle in monkeys. European Journal of Clinical Chemistry and Clinical Biochemistry 31, 633–637.Google ScholarPubMed
Munday, JS, Thompson, KG & James, KA (1999) Dietary conjugated linoleic acids promote fatty streak formations in the C57BL/6 mouse atherosclerosis model. British Journal of Nutrition 81, 251–255.CrossRefGoogle ScholarPubMed
Murakami, S, Araki, H, Otomo, S, Nara, Y & Yamori, Y (1995) Effect of HL-004, a novel ACAT inhibitor, on cholesterol accumulation and removal in cultured smooth muscle cells from stroke prone spontaneously hypertensive rats (SHRSP). Life Science 56, 509–520.CrossRefGoogle ScholarPubMed
Neaton, JD, Kuller, LH, Wentworth, D & Borhani, NO (1984) Total and cardiovascular mortality in relation to cigarette smoking, serum cholesterol concentration and diastolic blood pressure among black and white males followed up for five years. American Heart Journal 108, 759–769.CrossRefGoogle ScholarPubMed
Nicolosi, RJ, Rogers, EJ, Kritchevsky, D, Scimeca, JA & Huth, PJ (1997) Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery 22, 266–277.Google ScholarPubMed
Nistor, A, Bulla, A, Filip, DA & Radu, A (1987) The hypolipidemic hamsters as a model of experimental atherosclerosis. Atherosclerosis 68, 159–165.CrossRefGoogle Scholar
Park, Y, Albright, KL, Liu, W, Storkson, JM, Cook, ME & Pariza, MW (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32, 853–858.CrossRefGoogle ScholarPubMed
Park, Y, Albright, KL, Storkson, JM, Cook, ME & Pariza, MW (1999) Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid. Lipids 34, 243–248.CrossRefGoogle ScholarPubMed
Sanders, TA & Sandaradura, S (1992) The cholesterol-raising effect of coffee in the Syrian hamster. British Journal of Nutrition 68, 431–434.CrossRefGoogle ScholarPubMed
Sehat, N, Yurawecz, MP, Roach, JAG, Mossoba, MM, Kramer, JKG & Ku, Y (1998) Silver-ion high-performance liquid chromatographic separation and identification of conjugated linoleic acid isomers. Lipids 33, 217–221.CrossRefGoogle ScholarPubMed
Sessions, VA & Salter, AM (1994) The effect of different dietary fats and cholesterol on serum lipoprotein concentrations in hamsters. Biochimica et Biophysica Acta 1211, 207–214.CrossRefGoogle ScholarPubMed
Shapiro, DJ, Imblum, RL & Rodwell, VW (1969) Thin-layer chromatographic assay for HMG-CoA reductase and mevalonic acid. Analytical Biochemistry 31, 383–390.CrossRefGoogle ScholarPubMed
Sugiyama, Y, Odaka, H, Itokawa, S, Ishikawa, E, Tomari, Y & Ikeda, H (1995) TMP-153, a novel ACAT inhibitor, lowers plasma cholesterol through its hepatic action in Golden hamsters. Atherosclerosis 118, 145–151.CrossRefGoogle ScholarPubMed
Vegas, GL, Groszek, E, Wolf, R & Grundy, SM (1982) Influence of polyunsaturated fats on the composition of plasma lipoproteins and apolipoproteins. Journal of Lipid Research 23, 811–822.CrossRefGoogle Scholar
Wrenn, SM, Parks, JS, Immermann, FW & Rudel, LL (1995) ACAT inhibitors CL283,546 and CL283,796 reduce LDL cholesterol without affecting cholesterol absorption in African green monkeys. Journal of Lipid Research 36, 1199–1210.CrossRefGoogle Scholar
Yotsumoto, H, Hara, E, Naka, S, Adlof, RO, Emken, EA & Yanagita, T (1999) Trans-10, cis-12-linoleic acid reduces apolipoprotein B secretion in HepG2 cells. Food Research International 31, 403–409.CrossRefGoogle Scholar