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Dietary β-carotene inhibits mammary carcinogenesis in rats depending on dietary α-linolenic acid content

Published online by Cambridge University Press:  08 March 2007

Virginie Maillard ,
Claude Hoinard ,
Khelifa Arab ,
Marie-Lise Jourdan ,
Philippe Bougnoux  and
Véronique Chajès
Virginie Maillard
Affiliation:
Nutrition Croissance et Cancer, Université François-Rabelais de Tours, Inserm E 0211, IFR 135, 37032 Tours Cedex, France
Claude Hoinard
Affiliation:
Nutrition Croissance et Cancer, Université François-Rabelais de Tours, Inserm E 0211, IFR 135, 37032 Tours Cedex, France
Khelifa Arab
Affiliation:
Hôpital E. Herriot, Fed. Biochimie UF 206, 69437 Lyon cedex 03, France
Marie-Lise Jourdan
Affiliation:
Nutrition Croissance et Cancer, Université François-Rabelais de Tours, Inserm E 0211, IFR 135, 37032 Tours Cedex, France
Philippe Bougnoux*
Affiliation:
Nutrition Croissance et Cancer, Université François-Rabelais de Tours, Inserm E 0211, IFR 135, 37032 Tours Cedex, France
Véronique Chajès
Affiliation:
FRE 2939, Institut Gustave Roussy, 94805 Villejuif, France
*
*Corresponding author: Professor P. Bougnoux, fax +33 2 47 36 62 26, email [email protected]
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Abstract

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To investigate whether dietary α-linolenic acid (ALA) content alters the effect of β-carotene on mammary carcinogenesis, we conducted a chemically induced mammary tumorigenesis experiment in rats randomly assigned to four nutritional groups (15 rats per group) varying in β-carotene supplementation and ALA content. Two oil formula-enriched diets (15%) were used: one with 6g ALA/kg diet in an essential fatty acids (EFA) ratio of linoleic acid:ALA of 5:1 w/w (EFA 5 diet), the other with 24g ALA/kg diet in an EFA ratio of 1:1 w/w (EFA 1 diet), both designed with a similar linoleic acid content. β-Carotene was either added (10mg/kg diet per d) or not added to these diets. β-Carotene supplementation led to decreased tumour incidence and tumour growth when added to the EFA 5 diet, whereas it had no effect when added to the EFA 1 diet. The decreased tumour growth did not result from an involvement of lipoperoxidation (tumour malondialdehyde content being similar between the groups) or from an inhibition of tumour cell proliferation (as there was an unchanged S phase fraction in the tumours). We concluded that an adequate content of ALA in the diet is required to allow a protective effect of β-carotene in mammary carcinogenesis. Whether such an interaction between ALA and β-carotene influences the risk of breast cancer in women needs to be investigated.

Keywords

β-Caroteneα-Linolenic acidn-6:n-3 fatty acid ratioMammary tumours
Type
Short Communication
Information
British Journal of Nutrition , Volume 96 , Issue 1 , January 2006 , pp. 18 - 21
Copyright
Copyright © The Nutrition Society 2006

References

Alpha-Tocopherol Beta Carotene Cancer Prevention Study Group The effect of vitamin E and beta carotene on the incidence of lung cancer and other cancers in male smokers. N Engl J Med (1994) 330, 1029–1035.Google Scholar
Chew, BP & Park, JSCarotenoid action on the immune response. J Nutr (2004) 134, 257S–261S.CrossRefGoogle ScholarPubMed
Chew, BP, Park, JS, Wong, MW & Wong, TSA comparison of the anticancer activities of dietary beta-carotene, canthaxanthin and astaxanthin in mice in vivo. Anticancer Res (1999) 19, 1849–1853.Google ScholarPubMed
Cognault, S, Jourdan, ML, Germain, E, Pitavy, R, Morel, E, Durand, G, Bougnoux, P & Lhuillery, CEffect of an alpha-linolenic acid-rich diet on rat mammary tumor growth depends on the dietary oxidative status. Nutr Cancer (2000) 36, 33–41.Google Scholar
Colas, S, Paon, L, Denis, F, Prat, M, Louisot, P, Hoinard, C, Le Floch, O, Ogilvie, G & Bougnoux, PEnhanced radiosensitivity of rat autochthonous mammary tumors by dietary docosahexaenoic acid. Int J Cancer (2004) 109, 449–454.CrossRefGoogle ScholarPubMed
Hennekens, CH, Buring, JE, Manson, JE et al. Lack of effect of long-term supplementation with beta carotene on the incidence of malignant neoplasms and cardiovascular disease. N Engl J Med (1996) 334, 1145–1149.Google Scholar
IARC Working Group on the Evaluation of Cancer Preventive Agents In IARC Handbooks of Cancer Prevention, pp. 82–87 [IARC, editor]. Lyon: International Agency for Research on Cancer (1998a) Carotenoids.Google Scholar
IARC Working Group on the Evaluation of Cancer Preventive Agents In IARC Handbooks of Cancer Prevention, pp. 137–215 [IARC, editor]. Lyon: International Agency for Research on Cancer. (1998b) Carotenoids.Google Scholar
Lyan, B, Azais-Braesco, V, Cardinault, N, Tyssandier, V, Borel, P, Alexandre-Gouabau, MC & Grolier, PSimple method for clinical determination of 13 carotenoids in human plasma using an isocratic high-performance liquid chromatographic method. J Chromatogr B Biomed Sci Appl (2001) 751, 297–303.CrossRefGoogle ScholarPubMed
Omenn, GS, Goodman, GE, Thornquist, MD, et al. . Effects of a combination of beta carotene and vitamin A on lung cancer and cardiovascular disease. N Engl J Med (1996) 334, 1150–1155.CrossRefGoogle ScholarPubMed
Palozza, PProoxidant actions of carotenoids in biologic systems. Nutr Rev (1998) 56, 257–265.Google Scholar
Palozza, P, Calviello, G, Maggiano, N, Lanza, P, Ranelletti, FO & Bartoli, GMBeta-carotene antagonizes the effects of eicosapentaenoic acid on cell growth and lipid peroxidation in WiDr adenocarcinoma cells. Free Radic Biol Med (2000) 28, 228–234.Google Scholar
Palozza, P, Serini, S, Di Nicuolo, F & Calviello, GModulation of apoptotic signalling by carotenoids in cancer cells. Arch Biochem Biophys (2004) 430, 104–109.CrossRefGoogle ScholarPubMed
Potier de Courcy, G, Durand, G, Abraham, J & Gueguen, LRecommandations sur les conditions d'alimentation des animaux de laboratoire (rats et souris). Sci Aliments (1989) 9, 209–217.Google Scholar
Riboli, E & Norat, TEpidemiologic evidence of the protective effect of fruit and vegetables on cancer risk. Am J Clin Nutr (2003) 78, 559S–569S.CrossRefGoogle ScholarPubMed
Sies, H & Stahl, WVitamins E and C, beta-carotene, and other carotenoids as antioxidants. Am J Clin Nutr (1995) 62, 1315S–1321S.Google Scholar
Stahl, W, Ale-Agha, N & Polidori, MCNon-antioxidant properties of carotenoids. Biol Chem (2002) 383, 553–558.Google Scholar
Steghens, JP, van Kappel, AL, Denis, I & Collombel, CDiaminonaphtalene, a new highly specific reagent for HPLC-UV measurement of total and free malondialdehyde in human plasma or serum. Free Radic Biol Med (2001) 31, 242–249.CrossRefGoogle ScholarPubMed
Steghens, JP, van Kappel, AL, Riboli, E & Collombel, CSimultaneous measurement of seven carotenoids, retinol and alpha-tocopherol in serum by high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl (1997) 694, 71–81.Google Scholar