Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-29T19:41:47.735Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary amino acids: effect of depletion and recovery on synthesis in vitro in rat skeletal muscle and liver

Published online by Cambridge University Press:  07 January 2011

P. T. Omstedt  and
Alexandra Von Der Decken
P. T. Omstedt
Affiliation:
The Wenner-Gren Institute for Experimental Biology, Stockholm, Sweden
Alexandra Von Der Decken
Affiliation:
The Wenner-Gren Institute for Experimental Biology, Stockholm, Sweden
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Rats were given diets containing 200 g/kg of a complete or incomplete amino acid mixture or of high- or low-quality proteins. After 6 d the amino acid-incorporating activity of ribosomes from skeletal muscle and liver was studied.

2. The level of isotope incorporation relative to ribosomal RNA was similar for casein supplemented with methionine and for a complete amino acid mixture with the composition of whole-egg protein. Per wet weight of tissue there was a significant decrease after feeding with the complete amino acid mixture.

3. There was a significant decrease in activity after feeding with amino acid mixtures deficient in lysine, methionine or tryptophan. In skeletal muscle, but not in liver, the ribosomal activity was less than that obtained with wheat gluten. Activity per wet weight of both tissues was less than that obtained with wheat gluten.

4. Refeeding with methionine for 1 d resulted in complete restoration of ribosomal activity and activity per wet weight in skeletal muscle.

5. After lysine deficiency, protein synthesis per unit wet weight of both tissues and ribosomal activity in liver were not restored after 2 d of refeeding. Recovery of ribosomal activity in skeletal muscle was complete after 1 d.

6. Rats receiving the 200 g casein/kg diet supplemented with methionine at daily energy levels of 263, 176, 141 and 106 KJ (62.6, 42.1, 33.7 and 25.3 kcal) showed no changes in ribosomal activity, but there was a significant decrease in activity per wet weight when 106 KJ were given.

Type
Research Article
Information
British Journal of Nutrition , Volume 31 , Issue 1 , January 1974 , pp. 67 - 76
Copyright
Copyright © The Nutrition Society 1974

References

REFERENCES

Enwonwu, C. O. & Sreebny, L. M. (1971). J. Nutr. 101, 501.CrossRefGoogle Scholar
FAO (1970). FAO nutr. Stud. no. 24.Google Scholar
Harper, A. E., Benevenga, N. J. & Wohlhueter, R. M.(1970). Physiol. Rev. 50, 428.CrossRefGoogle Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). J. biol. Chem. 193, 265.CrossRefGoogle Scholar
Mans, R. J. & Novelli, G. D. (1961). Archs Biochem. Biophys. 94, 48.CrossRefGoogle Scholar
Morgan, W. S., von der Decken, A. & Hultin, T. (1961). Exp. Cell Res. 20, 655.CrossRefGoogle Scholar
Ogur, M. & Rosen, G. (1950). Archs Biocha. Biophys. 25, 262.Google Scholar
Omstedt, P. T. & von der Decken, A. (1972). Br. J. Nutr. 27, 467.CrossRefGoogle Scholar
Omstedt, P. T., von der Decken, A., Hedenskog, G. & Mogren, H. (1973). J. Sci. Fd Agric. 24, 1103.CrossRefGoogle Scholar
Peters, R. F., Richardson, M. C., Small, M. & White, A. M. (1970). Biochem. J. 116, 349.CrossRefGoogle Scholar
Sidransky, H., Wagle, D. S. & Verney, E. (1969). Lab. Invest. 20, 364.Google Scholar
Soeiro, R.& Amos, H. (1966). Science, N. Y. 154, 662.CrossRefGoogle Scholar
von der Decken, A. (1967). J. Cell Biol. 33, 657.CrossRefGoogle Scholar
von der Decken, A. (1968). Eur. J. Biochem. 4, 87.CrossRefGoogle Scholar
von der Decken, A. (1969). J. Cell Bid. 43, 138.CrossRefGoogle Scholar
von der Decken, A. & Omstedt, P. T. (1970). J. Nutr. 100, 623.CrossRefGoogle Scholar
von der Decken, A. & Omstedt, P. T. (1972). J. Nutr. 102, 1555.CrossRefGoogle Scholar
Waterlow, J. C.(1969). In Mammalian Protein Metabolism Vol. 3, p. 326 [Munro, H. N., editor]. New York and London: Academic Press.Google Scholar
Waterlow, J. C. & Stephen, J. M. L. (1968). Clin. Sci. 35, 287.Google Scholar
Wettstein, F. O., Staehelin, T. & Noll, H. (1963). Nature, Lond. 197, 430.CrossRefGoogle Scholar
Wretlind, A. (1972). Nutr. Metab. 14, Suppl. p. 1.CrossRefGoogle Scholar
Zimmermann-Nielsen, C. & Schmheyder, F. (1962). Biochim. biophys. Acta 63, 201.CrossRefGoogle Scholar