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Determination of each neutral oligosaccharide in the milk of Japanese women during the course of lactation

Published online by Cambridge University Press:  09 March 2007

Wataru Sumiyoshi ,
Tadasu Urashima ,
Tadashi Nakamura ,
Ikichi Arai ,
Tadao Saito ,
Norihiko Tsumura ,
Bing Wang ,
Janette Brand-Miller ,
Yoko Watanabe  and
Kazumasa Kimura
Wataru Sumiyoshi
Affiliation:
Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080-8555, Japan Course of the Science of Bioresources, The United Graduate School of Agricultural Science, Iwate University, 18-8 Ueda 3-chome, Morioka, Iwate, 020-8550, Japan
Tadasu Urashima*
Affiliation:
Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080-8555, Japan
Tadashi Nakamura
Affiliation:
Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080-8555, Japan
Ikichi Arai
Affiliation:
Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080-8555, Japan
Tadao Saito
Affiliation:
Department of Bioproduction, Graduate School of Agriculture, Tohoku University, Tsutsumidori-Amamiya machi 1-1, Aoba-ku, Sendai, Miyagi, 981-8555, Japan
Norihiko Tsumura
Affiliation:
Department of Obstetrics and Gynecology, Obihiro Kosei General Hospital, Obihiro, Hokkaido, 080-0016, Japan
Bing Wang
Affiliation:
Human Nutrition Unit, Department of Biochemistry, University of Sydney, NSW, 2006, Australia
Janette Brand-Miller
Affiliation:
Human Nutrition Unit, Department of Biochemistry, University of Sydney, NSW, 2006, Australia
Yoko Watanabe
Affiliation:
YAKULT Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo, 186-8650, Japan
Kazumasa Kimura
Affiliation:
YAKULT Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo, 186-8650, Japan
*
*Corresponding Author: Dr T. Urashima, fax +81 155 49 5577, email [email protected]
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Abstract

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Using reverse-phase HPLC after pyridylamination, we quantified the concentrations of major neutral oligosaccharides in the milk of sixteen Japanese women collected at 4, 10, 30 and 100 d postpartum. In colostrum and mature milk (30 d lactation), lacto-N-fucopentaose (LNFP) I was the most abundant oligosaccharide, followed by 2′-fucosyllactose (2′-FL) + lacto-N-difucotetraose (LNDFT), LNFP II + lacto-N-difucohexaose II (LNDFH II), and 3-fucosyllactose (3-FL). Together these accounted for 73 % of the total weight of neutral oligosaccharides in colostrum and mature milk. Changes in concentration occurred during the course of lactation. LNFP I and 2′-FL + LNDFT increased from 4 to 10 d postpartum, and then declined by 100 d. LNFP II + LNDFH II steadily increased during the first 30 d and then declined. In contrast, 3-FL increased steadily throughout the entire 100 d of study. Large differences were observed between our data and previously published data in Italian women, in terms of both the concentration and temporal changes of each oligosaccharide. These differences may be caused by different assay methodology, although racial differences cannot be ruled out.

Keywords

Human milk oligosaccharidesLactation periodPyridylaminationHigh-performance liquid chromatography
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 1 , January 2003 , pp. 61 - 69
Copyright
Copyright © The Nutrition Society 2003

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