Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-26T17:06:02.421Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of caseinophosphopeptides in the distal ileostomy fluid of human subjects

Published online by Cambridge University Press:  09 March 2007

H. Meisel ,
H. Meisel ,
S. Fairweather-Tait ,
R. J. FitzGerald ,
R. Hartmann ,
C. N. Lane ,
D. McDonagh ,
B. Teucher  and
J. M. Wal
H. Meisel*
Affiliation:
Federal Dairy Research Centre, Institute for Dairy Chemistry and Technology, Kiel, Germany
H. Meisel*
Affiliation:
Laboratoire d'Immuno-Allergie Alimentaire, INRA-CEA, SPI, Gif sur Yvette, France
S. Fairweather-Tait
Affiliation:
Institute of Food Research, Norwich Research Park, Norfolk, UK
R. J. FitzGerald
Affiliation:
University of Limerick, Life Sciences Department, Limerick, Republic of Ireland
R. Hartmann
Affiliation:
Federal Dairy Research Centre, Institute for Dairy Chemistry and Technology, Kiel, Germany
C. N. Lane
Affiliation:
Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Republic of Ireland
D. McDonagh
Affiliation:
Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Republic of Ireland
B. Teucher
Affiliation:
Institute of Food Research, Norwich Research Park, Norfolk, UK
J. M. Wal
Affiliation:
Laboratoire d'Immuno-Allergie Alimentaire, INRA-CEA, SPI, Gif sur Yvette, France
*
*Corresponding author: Professor Hans Meisel, fax +49 431 609 2300, email [email protected]
*Corresponding author: Professor Hans Meisel, fax +49 431 609 2300, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Caseinophosphopeptides (CPP) were detected for the first time in ileostomy fluid, collected at 2 h intervals for 10 h post milk and CPP ingestion, from human volunteers with an ileostomy. The level of CPP present in ileostomy fluid obtained from milk-fed volunteers was markedly higher than that from volunteers fed with selected CPP preparations. The findings are based on HPLC analysis in combination with peptide-bound P determination, thin-layer electrophoresis and amino acid analysis, together with ELISA studies using polyclonal antibodies raised against a set of CPP to detect immunoreactive CPP in ileostomy fluid. These procedures allowed the detection of nm concentrations of CPP. CPP, which can be released during intestinal digestion, may function as bioactive constituents and carriers for different minerals, especially Ca, and may be used as ingredients in functional foods or pharmaceutical preparations.

Keywords

CaseinophosphopeptidesMilkHuman ileostomy samplesBioactive peptides
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 3 , March 2003 , pp. 351 - 358
Copyright
Copyright © The Nutrition Society 2003

References

Adamson, NJ & Reynolds, EC (1995) Characterisation of tryptic casein phosphopeptides prepared under industrially relevant conditions. Biotechnology and Bioengineering 45, 196–204.CrossRefGoogle ScholarPubMed
Berrocal, R, Chanton, S, Juillerat, MA, Pavillard, B, Scherz, J-C & Jost, R (1989) Tryptic phosphopeptides from whole casein: II. Physicochemical properties related to the solubilisation of calcium. Journal of Dairy Research 56, 335–341.CrossRefGoogle Scholar
Borgström, B, Dahlqvist, A, Lundh, G & Sjövall, J (1957) Studies of intestinal digestion and absorption in the human. Journal of Clinical Investigation 36, 1521–1536.CrossRefGoogle ScholarPubMed
Bronner, F (1987) Intestinal calcium absorption: Mechanisms and applications. Journal of Nutrition 117, 1347–1352.CrossRefGoogle ScholarPubMed
Brule, G, Roger, L, Fauquant, J & Piot, M (1982) Phosphopeptides from casein-based material. U. S. Patent, No. 4,358,465.Google Scholar
Caruelle, D, Grassi, J, Courty, J, Groux-Muscatelli, B, Pradelles, P, Barritault, D & Caruelle, JP (1988) Development and testing of radio and enzyme immunoassays for acidic fibroblast growth factor. Analytical Biochemistry 173, 328–339.CrossRefGoogle ScholarPubMed
Chabance, B, Marteau, P, Rambaud, JC, Migliore-Samour, D, Boynard, M, Perrotin, P, Guillet, R, Jollès, P & Fiat, AM (1998) Casein peptide release and passage to the blood in humans during digestion of milk or yogurt. Biochimie 80, 155–165.CrossRefGoogle ScholarPubMed
Douglas, FW, Tobias, J, Groves, ML, Farrell, HM & Edmondson, LF (1982) Quantitative determination of total protein, casein, and whey protein of processed dairy products. Journal of Dairy Science 65, 33–45.CrossRefGoogle Scholar
Ellman, GL, Courtney, KD, Andres, V & Featherstone, RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 7, 88–95.CrossRefGoogle ScholarPubMed
Erba, D, Ciappellano, S & Testolin, G (2001) Effect of caseinphos-phopeptides on inhibition of calcium intestinal absorption due to phosphate. Nutrition Research 21, 649–656.CrossRefGoogle Scholar
Ferraretto, A, Signorile, A, Gravaghi, C, Fiorilli, A & Tettamanti, G (2001) Casein phosphopeptides influence calcium uptake by cultured human intestinal HT-29 tumor cells. Journal of Nutrition 131, 1655–1661.CrossRefGoogle ScholarPubMed
FitzGerald, RJ (1998) Potential uses of caseinophosphopeptides. International Dairy Journal 8, 451–457.CrossRefGoogle Scholar
FitzGerald, RJ & Meisel, H (2000) Milk protein-derived peptide inhibitors of angiotensin-I-converting enzyme. British Journal of Nutrition 84, Suppl. 1, 33–37.CrossRefGoogle ScholarPubMed
Hartmann, R, Günther, S, Martin, D, Meisel, H, Pentzien, A-K, Schlimme, E & Scholz, N (2000) Cytochemical model systems for the detection and characterization of potentially bioactive milk components. Kieler Milchwirtschaftliche Forschungsberichte 52, 61–85.Google Scholar
Hartmann, R & Meisel, H (2002) Cytochemical assessment of phosphopeptides derived from casein as potential ingredients for functional food. Nahrun/ood 46, 427–431.3.0.CO;2-P>CrossRefGoogle ScholarPubMed
Hata, I, Higashiyama, S & Otani, H (1998) Identification of a phosphopeptide in bovine αS1-casein digests as a factor influencing proliferation and immunoglobulin production in lymphocyte cultures. Journal of Dairy Research 65, 569–578.CrossRefGoogle Scholar
Hata, I, Ueda, J & Otani, H (1999) Immunostimulatory action of a commercially available casein phosphopeptide preparation, CPP-III, in cell cultures. Milchwissenschaft 54, 3–7.Google Scholar
McDonagh, D & FitzGerald, RJ (1998) Production of caseinophosphopeptides (CPP) from sodium caseinate using a range of commercial protease preparations. International Dairy Journal 8, 39–45.CrossRefGoogle Scholar
Meisel, H (1997) Biochemical properties of regulatory peptides derived from milk proteins. Biopolymers 43, 119–128.3.0.CO;2-Y>CrossRefGoogle ScholarPubMed
Meisel, H (1998) Overview on milk protein-derived peptides. International Dairy Journal 8, 363–373.CrossRefGoogle Scholar
Meisel, H (2001) Bioactive peptides from milk proteins: a perspective for consumers and producers. Australian Journal of Dairy Technology 56, 83–92.Google Scholar
Meisel, H, Bernard, H, Fairweather-Tait, S, FitzGerald, RJ, Hartmann, R, Lane, CN, McDonagh, D, Teucher, B & Wal, J-M (2001) Letter to the Editor: Nutraceutical and functional food ingredients for food and pharmaceutical applications. British Journal of Nutrition 85, 635.CrossRefGoogle Scholar
Meisel, H & FitzGerald, RJ (2000) Opioid peptides encrypted in intact milk protein sequences. British Journal of Nutrition 84 Suppl. 1, 27–31.CrossRefGoogle ScholarPubMed
Meisel, H & Frister, H (1988) Chemical characterization of a caseinophosphopeptide isolated from in vivo digests of a casein diet. Biological Chemistry Hoppe-Seyler 369, 1275–1279.CrossRefGoogle ScholarPubMed
Meisel, H & Olieman, C (1998) Estimation of calcium binding constants of casein phosphopeptides by capillary zone electrophoresis. Acta Chimica Acta 372, 291–297.CrossRefGoogle Scholar
Otani, H, Kihara, Y & Park, M (2000) The immunoenhancing property of a dietary casein phosphopeptide preparation in mice. Food and Agricultural Immunology 12, 165–173.CrossRefGoogle Scholar
Sato, R, Shindo, M, Gunshin, H, Noguchi, T & Naito, H (1991) Characterisation of phosphopeptide derived from bovine β-casein: An inhibitor to intra-intestinal precipitation of calcium phosphate. Biochimica et Biophysica Acta 1077, 413–415.CrossRefGoogle ScholarPubMed