Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-22T23:44:25.316Z Has data issue: false hasContentIssue false

Design and development of a long-term rumen simulation technique (Rusitec)

Published online by Cambridge University Press:  09 March 2007

J. W. Czerkawski
Affiliation:
The Hannah Research Institute, Ayr KA6 5HL, Scotland
Grace Breckenridge
Affiliation:
The Hannah Research Institute, Ayr KA6 5HL, Scotland
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The paper describes the development and construction of an apparatus for maintaining a normal microbial population of the rumen under strictly controlled conditions over long periods of time.

2. The apparatus is simple to construct and operate. It is possible to do four replicate experiments at the same time.

3. The results of three experiments are given. The experiments showed that when the steady-state was reached it could be maintained indefinitely, with the type and quantities of products of fermentation very similar to those in the rumen of donor animals, including the maintenance of normal protozoal populations for up to 49 d.

4. It was found that within wide ranges, the digestibility of rations and the output of products were independent of dilution rate.

5. Except for the lowest ‘level of feeding’, the digestibility was independent of the level of feeding. The output of products was proportional to the amount of food digested and was the same as would be expected in sheep on similar rations.

6. An experiment in which a ration of hay was changed to a mainly concentrate ration showed that the fermentation characteristics were determined mainly by the food given.

Type
Research Article
Copyright
Copyright © The Nutrition Society 1977

References

REFERENCES

Aafjes, J. H. & Nijhof, J. K. (1967). Br. vet. J. 123, 436.CrossRefGoogle Scholar
Abe, M. & Kumeno, F. (1973). J. Anim. Sci. 36, 941.CrossRefGoogle Scholar
Cottyn, B. G. & Boucque, C. F. (1968). J. agric. Fd Chem. 16, 105.CrossRefGoogle Scholar
Czerkawski, J. W. (1974). J. Sci. Fd Agric. 25, 45.CrossRefGoogle Scholar
Czerkawski, J. W. (1976 a). Lab. Prac. 25, 15.Google Scholar
Czerkawski, J. W. (1976 b). Proceedings of the 7th Symposium on Energy Metabolism, Vichy, France, p. 355. Clermont-Ferrand: G. D. Bussac.Google Scholar
Czerkawski, J. W. (1976 c). J. Sci. Fd Agric. 27, 323.CrossRefGoogle Scholar
Czerkawski, J. W. & Breckenridge, G. (1969). Br. J. Nutr. 23, 51.CrossRefGoogle Scholar
Czerkawski, J. W. & Breckenridge, G. (1970). Lab. Prac. 19, 717.Google Scholar
Czerkawski, J. W., Christie, W. W., Breckenridge, G. & Hunter, M. L. (1975). Br. J. Nutr. 34, 25.CrossRefGoogle Scholar
Czerkawski, J. W. & Clapperton, J. L. (1968). Lab. Prac. 17, 994.Google Scholar
Gray, F. V., Weller, A. F., Pilgrim, A. F. & Jones, G. E. (1962). Aust. J. agric. Res. 13, 343.CrossRefGoogle Scholar
Harrison, D. G., Beever, D. E., Thomson, D. J. & Osbourn, D. F. (1975). J. agric. Sci., Camb. 85, 93.CrossRefGoogle Scholar
Hoover, W. H., Crooker, B. A. & Sniffen, C. J. (1976). J. Anim. Sci. 43, 525.Google Scholar
Hoover, W. H., Knowlton, P. H., Stern, M. D. & Sniffen, C. J., (1976). J. Anim. Sci. 43, 535.CrossRefGoogle Scholar
Isaacson, H. R., Hinds, F. C., Bryant, M. P. & Owens, F. N. (1975). J. Dairy Sci. 58, 1645.CrossRefGoogle Scholar
Jacobs, S. (1960). Analyst, Lond. 85, 257.CrossRefGoogle Scholar
Latham, M. J. & Sharpe, M. E. (1975). Proc. Nutr. Soc. 34, 113A.Google Scholar
McDougall, E. I. (1948). Biochem. J. 43, 99.CrossRefGoogle Scholar
Slyter, L. L., Nelson, W. O. & Wolin, M. J. (1964). Appl. Microbiol. 12, 374.CrossRefGoogle Scholar
Spies, J. R. (1952). J. biol. Chem. 195, 65.CrossRefGoogle Scholar
Thomson, D. J., Beever, D. E., Mundell, D. C., Elderfield, M. L. & Harrison, D. G. (1975). Proc. Nutr. Soc. 34, 111A.Google Scholar
Toennies, G. & Feng, F. (1965). Analyt. Biochem. 11, 411.CrossRefGoogle Scholar