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Consumption of diets containing raw soya beans (Glycine max), kidney beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata) or lupin seeds (Lupinus angustifolius) by rats for up to 700 days: effect on body composition and organ weights

Published online by Cambridge University Press:  09 March 2007

Grorge Grant ,
Patrica M. Dorward ,
Wendy C. Buchan ,
Julia C. Armour  and
Arpad Pusztai
Grorge Grant
Affiliation:
Rowettt Research Institute, Bucksburn, Aberdeen AB2 9SB
Patrica M. Dorward
Affiliation:
Rowettt Research Institute, Bucksburn, Aberdeen AB2 9SB
Wendy C. Buchan
Affiliation:
Rowettt Research Institute, Bucksburn, Aberdeen AB2 9SB
Julia C. Armour
Affiliation:
Rowettt Research Institute, Bucksburn, Aberdeen AB2 9SB
Arpad Pusztai
Affiliation:
Rowettt Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

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Feeding trials have been done with rats to assess the effects of long-term (700 d) consumption of diets based on raw cowpeas (Vigna unguiculata; moderate Bowman–Birk inhibitor content, low lectin content), lupin seeds (Lupinus angustifolius; low lectin and protease inhibitor content) or soya beans (Glycine max; high Kunitz inhibitor content, moderate Bowman–Birk inhibitor content, moderate lectin content) or diets containing low levels of raw kidney bean (Phaseolus vulgaris; high lectin content, low Bowman–Birk inhibitor content) on body weight and composition and organ weights. All the legume-based diets reduced feed conversion efficiency and growth rates during the initial 250 d. However, after 250 d the weight gains by rats given legume-based diets were similar to those of controls given the same daily feed intake. Long-term consumption of diets containing low levels of kidney bean significantly altered body composition of rats. The levels of lipid in the body were significantly reduced. As a result, carcasses of these rats contained a higher proportion of muscle/protein than did controls. Small-intestine relative weight was increased by short- and long-term consumption of the kidney-bean-based diet. However, the increase in relative pancreatic weight observed at 30d did not persist long term. None of the other legume-based diets caused any significant changes in body composition. However, long-term exposure to a soya-bean- or cowpea-based diet induced an extensive increase in the relative and absolute weights of the pancreas and caused an increase in the incidence of macroscopic pancreatic nodules and possibly pancreatic neoplasia. Long-term consumption of the cowpea-, kidney-bean-, lupin-seed- or soya-bean-based diets by rats resulted in a significant increase in the relative weight of the caecum and colon.

Keywords

LegumesRatBody compositionGastrointestinal tractPancreas
Type
Dietary effects of bean consuption
Information
British Journal of Nutrition , Volume 73 , Issue 1 , January 1995 , pp. 17 - 29
Copyright
Copyright © The Nutrition Society 1995

References

REFERENCES

Booth, A. N., Robbins, W. E., Ribelin, W. E, de Eds, F., Smith, A. K & Rackis, J. J. (1964) Prolonged pancreatic hypertrophy and reversibility in rats fed raw soybean meal. Proceedings of the Society for Experimental Biology and Medicine 116, 1067–1069.CrossRefGoogle ScholarPubMed
Carvalho, A. de, F., F. U. (1993) Dietary kidney bean lectins affect insulin levels, change gene expression and modulate metabolism. PhD Thesis, University of Aberdeen.Google Scholar
Chase, T. & Shaw, E. (1967). p-Nitrophenyl-p'-guanidinobenzoate HCl: a new active site titrant for trypsin. Biochemical and Biophysical Research Communications 29, 508–514.CrossRefGoogle ScholarPubMed
Davidson, J., Mathieson, J. & Boyne, A.W. (1970) The use of automation in determining nitrogen by the kjeldahl method with final calculations by computer. Analyst 95, 181–193.CrossRefGoogle ScholarPubMed
de Oliveira, J. T. A. (1986) Seed lectins – the effects of dietary (Phaseolus vulgaris) lectins on general metabolism of monogastric animals. PhD Thesis, University of Aberdeen.Google Scholar
de Oliveira, J. T. A., Grant, G. & Pusztai, A. (1988) Changes in organs and tissues induced by feeding of purified kidney bean (Phaseolus vu1garis) lectins. Nutrition Research 8, 943–947.CrossRefGoogle Scholar
Erlanger, F. B., Cooper, A. J. & Bendich, A. J (1964) On the heterogeneity of three-times crystallised?- Chymotrypsin. Biochemistry 3, 1880–1883.CrossRefGoogle ScholarPubMed
Grant, G. (1989) Anti-nutritional effects of soyabean: a review. Progress in Food and Nutrition Science 13, 317–348.Google ScholarPubMed
Grant, G. (1990) Effects of long-term (2 year) feeding of rats with raw soya bean. Proceedings of the Nutrition Society 49, 143A.Google Scholar
Grant, G. (1991) Lectins. In Toxic Substances in Crop Plants, pp. 49–67 [D'Mello, J.P. F., Duffus, C. M. and Duffus, J. H., editors]. Cambridge: Royal Society of Chemistry.CrossRefGoogle Scholar
Grant, G., de Oliveira, J. T. A., Dorward, P. M., Annand, M. G., Waldron, M. & Pusztai, A. (1988) Metabolic and hormonal changes in rats resulting from consumption of kidney bean (Phaseolus vulgaris) or soyabean (Glycine max). Nutrition Reports Internationa 136, 763–772.Google Scholar
Grant, G., Dorward, P. M. & Pusztai, A. (1993) Pancreatic enlargement is evident in rats fed diets containing raw soybeans (Glycine max) or cowpeas (Vigna unguiculata) for 800 days but not in those fed diets based on kidney beans (Phaseolus vulgaris) or lupinseed (Lupinus angustifolius). Journal of Nutrition 123, 2207–2215.CrossRefGoogle Scholar
Grant, G., Ewen, S. W. B., Bardocz, S., Brown, D. S., Dorward, P. M., Watt, W. B., Stewart, J. C & Pusztai, A. (1989) Local (gut) and systemic response of rats to dietary soyabean (Glycine max) proteins. In Recent Advances of Research in Antinutritional Factors in Legume Seeds, pp. 34–38 [Huisman, J.van der Poel, T. F. B. and Liener, I. E. editors]. Wageningen: Pudoc.Google Scholar
Grant, G., McKenzie, N. H., Watt, W. B., Stewart, J. C., Donvard, P. M. & Pusztai, A. (1986) Nutritional evaluation of soya beans (Glycine max): nitrogen balance and fractionation studies. Journal of the Science of Food and Agriculture 37, 1001–1010.CrossRefGoogle Scholar
Grant, G., More, L. J., McKenzie, N. H., Stewart, J. C. & Pusztai, A.(1983) A survey of the nutritional and haemagglutinating properties of legume seeds generally available in the UK. British Journal of Nutrition 50, 207–214.CrossRefGoogle ScholarPubMed
Grant, G., Watt, W. B., Stewart, J. C & Pusztai, A. (1987 a) Effects of dietary soyabean (Glycine max) lectin and trypsin inhibitors upon the pancreas of rats. Medical Science Research 15, 1197–1198.Google Scholar
Grant, G., Watt, W. B., Stewart, J. C & Pusztai, A. (1987 b) Changes in the small intestine and hind-leg muscle of rats induced by dietary soyabean (Glycine mux) proteins. Medical Science Research 15, 1355–1356.Google Scholar
Gumbmann, M. R., Dugan, G. M., Spangler, W. L., Baker, E. C. & Rakis, J. J. (1989) Pancreatic response in rats and mice to trypsin inhibitors from soy and potato after short- and long-term dietary exposure. Journal of Nutrition 119, 1589–1609.CrossRefGoogle Scholar
Gumbmann, M. R., Spangler, W. L., Dugan, G. M., Rackis, J. J. & Liener, I. E. (1985) The USDA trypsin inhibitor study. IV. The chronic eflects of soy flour and soy protein isolate on the pancreas of rats after two years Qualitas Plantarum. Plant Foods for Human Nutrition 35, 275–314.CrossRefGoogle Scholar
Kakade, M. L., Simons, N. & Liener, I. E. (1969) An evaluation of natural vs synthetic substrates for measuring the antitryptic activity of soyabean samples. Cereal Chemistry 46, 518–526.Google Scholar
Kim, C. S. & Madhusudhan, K. T. (1988) Haemagglutinating and trypsin inhibitor activities of lupin seeds (Lupinus angustijolius). Journal of the Science of Food and Agriculture 53, 1234–1235.Google Scholar
Liener, I. E. (editor) (1980) Toxic Constituents of Plant Foodstuffs New York: Academic Press.Google Scholar
Liener, I. E. (1989) Control of antinutritional and toxic factors in oilseeds and legumes. In Food Uses of Whole Oil and Protein Seeds, pp, 344–371 [Lusas, E.W., Erickson, D. R. and Nip, W., editors]. Illinois: American Oil Chemists Society.Google Scholar
Longnecker, D. S. (1989) Experimental pancreatic carcinogenesis. Current Opinion in Gastroenterology 5, 723–727.CrossRefGoogle Scholar
McGuiness, E. E., Morgan, R. G. H., Levison, D. A., Frape, D. L., Hopwood, D. & Wormsley, K. G. (1980) The effects of long-term feeding of soya flour upon the rat pancreas. Scandinavian Journal of Gastroenterology 15, 497–502.CrossRefGoogle Scholar
McGuiness, E. E., Morgan, R. G. H. & Wormsley, K. G. (1984) Effects of soyabean flour upon the pancreas of rats. Environmental Health Perspectives 56, 205–212.CrossRefGoogle Scholar
Mathers, J. C., Kennard, J. & James, O. F. W. (1993) Gastrointestinal responses to oats consumption in young and elderly rats: digestion, Iarge bowel fermentation and crypt cell proliferation rates. British Journal of Mutrition 70, 567–584.CrossRefGoogle Scholar
Norton, G. (1991) Proteinase inhibitors. In Toxic Substances in Crop Plants, pp. 68–106 [D'Mello, J.P. F., Duffus, C. M. and Duffus, J. H., editors]. Cambridge: Royal Society of Chemistry.CrossRefGoogle Scholar
Pusztai, A. (1966) The isolation of two proteins, glycoprotein I and a trypsin inhibitor, from the seeds of kidney bean (Phaseolus vulgaris). Biochemical Journal 101, 379–384.CrossRefGoogle Scholar
Pusztai, A. (1980) Nutritional toxicity of kidney bean (Phaseolus vulgaris). Report of the Rowett Research Institute 36, 110–118.Google Scholar
Pusztai, A. (1989) Biological effects of dietary lectins. In Recent Advances of Research in Antinutritional Factors in Legume Seeds, pp. 17–29 [Huisman, J., van der Poel, T. F.B. & Liener, I. E., editors]. Wageningen: Pudoc.Google Scholar
Pusztai, A. (editor) (1991) Plant Lectirs. Cambridge: Cambridge University Press.Google Scholar
Pusztai, A., Ewen, S. W. B., Carvalho, A. de, F., F. U., Grant, G., Stewart, J. C. & Bardocz, S. (1991 a) Immuneand hormonal effects of dietary lectins. In European Food Toxicology. III. Proceedings of the Interdisciplinary Conference on Efects of Food on the Immune and Hormonal Systems, pp. 20–24. Zurich, Switzerland: University of Zurich.Google Scholar
Pusztai, A., Ewen, S. W. B., Grant, G., Peumans, W. P., van Damme, E. J. M., Rubio, L. & Bardocz, S. (1990) Relationship between survival and binding of plant lectins during small intestinal passage and their effectiveness as growth factors. Digestion 46, Suppl., 308–316.CrossRefGoogle ScholarPubMed
Pusztai, A., Grant, G., Brown, D. J., Stewart, J. C., Bardocz, S., Ewen, S. W. B., Gatehouse, A. M. R. & Hilder, V. (1992) Nutritional evaluation of the trypsin (EC 3.4.21.4) inhibitor from cowpea (Vigna unguiculataWalp) British Journal of Nutrition 68, 783–791.CrossRefGoogle Scholar
Pusztai, A., Grant, G. & de Oliveira, J. T. A. (1986) Local (gut) and systemic responses to dietary lectins. IRCS Medical Science 14, 205–208.Google Scholar
Pusztai, A., Greer, F. & Grant, G. (1989) Specific uptake of dietary lectins into the systemic circulation of rats. Biochemical Society Transactions 17, 481–432.CrossRefGoogle Scholar
Pusztai, A. & Palmer, R. (1977) Nutritional evaluation of the kidney beans (Phaseolus vulgaris): the toxic principle. Journal of the Science of Food and Agriculture 28, 620–623.CrossRefGoogle Scholar
Pusztai, A., Watt, W. B. & Stewart, J. C. (1991 b) A comprehensive scheme for the isolation of trypsin inhibitors and the agglutinin from soyabean seeds. Journal of Agricultural and Food Chemistry 39, 862–866.CrossRefGoogle Scholar
Rackis, J. J. (1974) Biological and physiological factors in soyabeans. Journal of the American Oil Chemists Society 51, 161A–174A.CrossRefGoogle Scholar
Rogers, A. E. (1979) Nutrition. In The Laboratory Rat Vol. 1 Biology and Diseases, pp. 123–152 [Baker, H. J., Linsdsey, J. R., and Weisbroth, S. H., editors]. New York: Academic Press.CrossRefGoogle Scholar
Saxena, H. C., Jensen, L. S. & McGinnis, J. (1963) Influence of age on the utilization of soybean meal by Chickens. Journal of Nutrition 80, 391–396.CrossRefGoogle ScholarPubMed
Schonbaum, G. R., Zerner, B. & Bender, M. L. (1961) The spectrophotometric determination of the operational normality of an α-chymotrypsin solution. Journal of Biological Chemistry 236, 2930–2935.CrossRefGoogle ScholarPubMed
Vellas, B. J., Balas, D., Lafont, C., Senegas-Balas, F., Albarede, J. & Ribet, A. (1990) Adaptive response of pancreatic and intestinal function to nutritional intake in the aged. Journal of the American Geriatric Society 38, 254–258.CrossRefGoogle ScholarPubMed
Zar, J. H. (editor) (1984) Biostatistical Analysis. Englewood Cliffs, NJ: Prentice-Hall Inc.Google Scholar