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Conjugated linoleic acid enhanced the immune function in broiler chicks

Published online by Cambridge University Press:  08 March 2007

Haijun Zhang ,
Yuming Guo  and
Jianmin Yuan
Haijun Zhang
Affiliation:
Department of Animal Nutrition & Feed Science, College of Animal Science & Technology, China Agricultural University, Beijing 100094, P. R.China
Yuming Guo*
Affiliation:
Department of Animal Nutrition & Feed Science, College of Animal Science & Technology, China Agricultural University, Beijing 100094, P. R.China
Jianmin Yuan
Affiliation:
Department of Animal Nutrition & Feed Science, College of Animal Science & Technology, China Agricultural University, Beijing 100094, P. R.China
*
*Corresponding author: Dr Yuming Guo, fax +86 10 62733900, email [email protected]
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Abstract

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This study was undertaken to investigate the growth performance and immune responses of broiler chicks fed diets supplemented with conjugated linoleic acid (CLA). Two hundred and forty day-old Arbor Acre male broiler chicks were randomly allotted into four dietary treatments with different inclusion levels of CLA (0, 2·5, 5·0 or 10·0g pure CLA/kg) for 6 weeks. Growth performance, lysozyme activity, peripheral blood mononuclear cell (PBMC) proliferation, prostaglandin E2 (PGE2) synthesis and antibody production were investigated. There were no significant differences in growth performance among treatments (P>0·05). Chicks fed 10·0g CLA/kg diet produced 40% and 49% more lysozyme activity in serum and spleen than the control group at 21d of age (P<0·05). Dietary CLA enhanced the PBMC proliferation in response to concanavalin A at the age of 21 and 42d (P<0·05). Systemic and peripheral blood lymphocytic synthesis of PGE2 in chicks fed 10·0g CLA/kg diet was significantly decreased by 57% and 42% compared to chicks fed control diet (P<0·05). Antibody production to sheep red blood cell and bovine serum albumin were elevated in either 2·5 or 10·0g CLA/kg dietary treatments (P<0·05). The results indicated dietary CLA could enhance the immune response in broiler chicks, but did not alter the growth performance.

Keywords

Conjugated linoleic acidBroiler chickensGrowthAntibody productionProstaglandin E2
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 5 , November 2005 , pp. 746 - 752
Copyright
Copyright © The Nutrition Society 2005

References

An, BK, Shin, KH, Kobayashi, Y, Tanaka, K & Wang, CW (2003) Excessive dietary conjugated linoleic acid affects hepatic lipid content and muscular fatty acid composition in young chicks. Asian-Aust J Anim Sci 16, 1171–1176.CrossRefGoogle Scholar
Badinga, L, Selberg, KT, Dinges, AC, Comer, CW & Miles, RD (2003) Dietary conjugated linoleic acid alters hepatic lipid content and fatty acid composition in broiler chickens. Poult Sci 82, 111–116.CrossRefGoogle ScholarPubMed
Banni, S, Angioni, E, Casu, V, Melis, MP, Carta, G, Corongiu, FP, Uhompson, H & Ip, C (1999) Decrease in linoleic acid metabolites as a potential mechanism in cancer risk reduction by conjugated linoleic acid. Carcinogenesis 20, 1019–1024.CrossRefGoogle ScholarPubMed
Bassaganya-Riera, J, Hontecillas-Magarzo, Bregendahl RK, Wannemuehler, MJ & Zimmerman, DR (2001) Effects of dietary conjugated linoleic acid in nursery pigs of dirty and clean environments on growth, empty body composition, and immune competence. J Anim Sci 79, 714–721.CrossRefGoogle ScholarPubMed
Belury, MA (2002) Dietary conjugated linoleic acid in health: physiological effects and mechanisms of action. Annu Rev Nutr 22, 505–531.CrossRefGoogle ScholarPubMed
Belury, MA & Kempa-steczko, A (1997) Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 32, 199–204.CrossRefGoogle ScholarPubMed
Betz, M & Fox, BS (1991) Prostaglandin E2 inhibits production of Th1 lymphokines but not of Th2 lymphokines. J Immunol 146, 108–113.CrossRefGoogle Scholar
Chin, SF, Storkson, JM, Albright, YL, Cook, ME & Pariza, MW (1994) Conjugated linoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed effieciency. J Nutr 124, 2344–2349.CrossRefGoogle Scholar
Chew, BP, Wong, TS, Shultz, TD & Magnuson, NS (1997) Effects of conjugated dienoic derivative of linoleic acid and beta-carotene in modulating lymphocyte and macrophage function. Anticancer Res 17, 1099–1106.Google ScholarPubMed
Cook, ME, Miller, CC, Park, Y & Pariza, MW (1993) Immune modulation by altered nutrient metabolism: nutritional control of immune-induced growth depression. Poult Sci 72, 1301–1305.CrossRefGoogle ScholarPubMed
Du, M & Ahn, DU (2002) Effect of dietary linoleic acid on the growth rate of live birds and on the abdominal fat content and quality of broiler meat. Poult Sci 81, 428–433.CrossRefGoogle ScholarPubMed
Dugan, MER, Aalhus, JL, Schaefer, AL & Kramer, JKG (1997) The effects of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Can J Anim Sci 77, 723–725.CrossRefGoogle Scholar
Guo, YM, Chen, SY, Xia, ZG & Yuan, JM (2004) Effects of different types of polyunsaturated fatty acids on immune function and PGE 2 synthesis by peripheral blood leukocytes of laying hens. Anim Feed Sci Technol 116, 249–257.CrossRefGoogle Scholar
Hasler, F, Bluestein, HG, Zvaifler, NJ & Epstein, LB (1983) Analysis of the defects responsible for the impaired regulation of EVB-induced B cell proliferation by rheumatoid arthritis lymphocytes. Role of monocytes and the increased sensitivity of rheumatoid arthritis lymphocytes to prostaglandin E. J Immunol 131, 768–772.CrossRefGoogle ScholarPubMed
Hayek, MG, Han, SN, Wu, D, Watkins, BA, Meydani, M, Dorsey, JL, Smith, DE & Meydani, SN (1999) Dietary conjugated linoleic acid influences the immune response of young and old C57BL/bNCrlBR mice. J Nutr 129, 32–38.CrossRefGoogle Scholar
Hontecillas, R, Wannemeulher, MJ, Zimmerman, DR, Hutto, DL, Wilson, JH, Ahn, DU & Bassaganya-Riera, J (2002) Nutritional regulation of porcine bacterial-induced colitis by conjugated linoleic acid. J Nutr 132, 2019–2027.CrossRefGoogle ScholarPubMed
Ip, C, Chin, F, Scimeca, JA & Pariza, MW (1991) Mammary cancer prevention by conjugated derivatives of linoleic acid. Cancer Res 51, 6118–6124.Google ScholarPubMed
Isakov, N, Feldmann, M & Segal, S (1982) The mechanism of modulation of humoral immune responses after injection of mice with lactic dehydrogenase virus. J Immunol 128, 969–975.CrossRefGoogle Scholar
Kelly, DS, Warren, JM, Simon, VA, Bartolini, G, Mackey, BE & Erickson, KL (2002) Similar effects of c9, t11-CLA and t10, c12-CLA on immune cell functions in mice. Lipids 37, 725–728.CrossRefGoogle Scholar
Kreukniet, MB, Nieuwland, BMG, Van der Zijpp, AJ (1994) Phagocytic activity of two lines of chickens divergently selected for antibody production. Vet Immunol Immunopathol 44, 377–387.CrossRefGoogle Scholar
Lee, K, Kritchevsky, D & Pariza, MW (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 19–25.CrossRefGoogle ScholarPubMed
Li, Y & Watkins, BA (1998) Conjugated linoleic acids alter bone fatty acid composition and reduce ex vivo prostaglandin E2 biosynthesis in rats fed n-6 or n-3 fatty acid. Lipids 33, 417–425.CrossRefGoogle ScholarPubMed
Lin, QH (1999) Lymphocytes transformation test. In Methods of Immune Research, pp. 188–190 [Lin, QH, editor], Wuhan, China: Wuhan University Press (in Chinese).Google Scholar
Liu, KL & Belury, MA (1997) Conjugated linoleic acid modulation of phorbol ester-induced events in murine keratinocyete. Lipids 32, 725–730.CrossRefGoogle Scholar
Melnick, DA, Nauseef, WM, Markowitz, SD, Gardner, JP & Malech, HL (1985) Biochemical analysis and subcellular localization of a neutrophil-specific antigen, PMN-7, involved in the respiratory burst. J Immunol 134, 3346–3355.CrossRefGoogle ScholarPubMed
Michal, JJ, Chew, BP, Schultz, TD, Wong, TS & Magnuson, NS (1992) Interaction of conjugated dienoic derivatives of linoleic acid with β-carotene on cellular host defense. FASEB J 6, A1102.Google Scholar
Miller, CC, Park, Y, Pariza, MW & Cook, ME (1994) Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biochem Biophys Res Commun 198, 1107–1112.CrossRefGoogle ScholarPubMed
Mosmann, T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods 65, 55–63.CrossRefGoogle Scholar
O'Shea, M, Bassaganya-Riera, J & Mohede, ICM (2004) Immunomodulatory properties of conjugated linoleic acid. Am J Clin Nutr 79, 1199S–1206S.CrossRefGoogle ScholarPubMed
Ostrowska, E, Muralitharan, M, Cross, RF, Bauman, DE & Dunshea, FR (1999) Dietary conjugated linoleic acid increase lean tissue and decrease fat deposition in growing pigs. J Nutr 129, 2037–2042.CrossRefGoogle ScholarPubMed
Pariza, MW, Park, Y & Cook, ME (2000) Mechanisms of action of conjugated linoleic acid: evidence and speculation. Proc Soc Exp Biol Med 223, 8–13.Google ScholarPubMed
Pariza, MW, Park, Y & Cook, ME (2001) The biologically active isomers of conjugated linoleic acid. Prog Lipid Res 40, 283–289.CrossRefGoogle ScholarPubMed
Simon, O, Manner, K, Schafer, K, Sagredos, A & Eder, K (2000) Effects of conjugated linoleic acids on protein to fat proportions, fatty acids, and plasma lipids in broiler. Eur J Lipid Sci Technol 102, 402–410.3.0.CO;2-T>CrossRefGoogle Scholar
Sugano, M, Tsujita, A, Yamasaki, M, Noguchi, M & Yamada, K (1998) Conjugated linoleic acid modulates tissue levels of chemical mediators and immunoglobulins in rats. Lipids 33, 521–527.CrossRefGoogle ScholarPubMed
Sugano, M, Tsujita, A, Yamasaki, M, Yamada, K, Ikeda, I & Kritchevsky, D (1997) Lymphatic recovery, tissue distribution, and metabolic effects of conjugated linoleic acid in rats. J Nutr Biochem 8, 38–43.CrossRefGoogle Scholar
Szymczyk, B, Pisulewski, P, Szczurek, W & Hanczakowski, P (2000) The effects of feeding conjugated linoleic acid on rat growth performance, serum lipoproteins and subsequent lipid composition of selected rat tissues. J Sci Food Agric 80, 1553–1558.3.0.CO;2-Z>CrossRefGoogle Scholar
Szymczyk, B, Pisulewski, P, Szczurek, W & Hanczakowski, P (2001) Effects of dietary conjugated linoleic acid on growth performance, feed conversion efficiency, and subsequent carcass quality in broiler chickens. Br J Nutr 85, 465–473.CrossRefGoogle ScholarPubMed
Takahashi, K, Akiba, Y, Iwata, T & Masaaki, K (2003) Effect of a mixture of conjugated linoleic acid isomers on growth performance and antibody production in broiler chicks. Br J Nutr 89, 691–694.CrossRefGoogle ScholarPubMed
Truitt, A, McNeill, G & Vanderhoek, JY (1999) Antiplatelet effects of conjugated linoleic acid isomers. Biochim Biophys Acta Mol Cell Biol Lipids 1438, 239–246.CrossRefGoogle ScholarPubMed
Turek, JJ, Li, Y, Schoenlein, IA, Allen, KGD & Watkins, BA (1998) Modulation of macrophage cytokine production by conjugated linoleic acids is influenced by the dietary n-6:n-3 fatty acid ratio. J Nutr Biochem 9, 258–266.CrossRefGoogle Scholar
Twibell, RG, Watkins, BA, Rogers, L & Brown, PB (2000) Effects of dietary conjugated linoleic acids on hepatic and muscle lipids in hybrid striped bass. Lipids 35, 155–161.CrossRefGoogle ScholarPubMed
Wang, CL (2000) The Guide to Practical Management of Birds in China, pp. 192–198. Shanghai: Shanghai Science and Technology Press, (in Chinese).Google Scholar
Wang, X, Ma, G, Zheng, B & Tian, H (1995) Effects of SL-probiotic preparation on the body weight and phagocytosis of white mice. Wei Sheng Wu Xue Bao 35, 455–459.Google ScholarPubMed
West, DB, Delany, JP, Camet, PM, Blohm, F, Truett, AA & Scimeca, J (1998) Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am J Physiol 275, R667–R672.Google ScholarPubMed
Whigham, LD, Cook, EB, Stahl, JL, Saban, R, Bjorling, DE, Pariza, MW & Cook, ME (2001) CLA reduces antigen-induced histamine and PGE(2) release from sensitized guinea pig tracheae. Am J Physiol Reg 280, R908–R912.Google ScholarPubMed
Whigham, LD, Higbee, A, Bjorling, DE, Park, YH, Pariza, MW & Cook, ME (2002) Decreased antigen-induced eicosanoid release in conjugated linoleic acid-fed guinea pigs. Am J Physiol Regul Integr Comp Physiol 282, R1104–R1112.CrossRefGoogle ScholarPubMed
Wong, MW, Chew, BP, Wong, TS, Hosick, HL, Boylston, TD & Shultz, TD (1997) Effects of dietary conjugated linoleic acid on lymphocyte function and growth of mammary tumors in mice. Anticancer Res 17, 987–993.Google ScholarPubMed
Xia, ZG, Guo, YM, Chen, SY & Yuan, JM (2003) Effects of dietary polyunsaturated fatty acids on antibody production and lymphocyte proliferation of laying hens. Asian-Aust J Anim Sci 16, 1320–1325.CrossRefGoogle Scholar
Yamasaki, M, Ikeda, A, Oji, M, Tanaka, Y, Hirao, A, Kasai, M, Iwata, T, Tachibana, H & Yamada, K (2003) Modulation of body fat and serum leptin levels by dietary conjugated linoleic acid in Sprague Dawley rats fed various fat-level diets. Nutrition 19, 30–35.CrossRefGoogle ScholarPubMed
Yamasaki, M, Kishihara, K, Mansho, K, Ogino, Y, Kasai, M, Sugano, M, Tachibana, H & Yamada, K (2000) Dietary conjugated linoleic acid increases immunoglobulin productivity of Sprague-Dawley rat spleen lymphocytes. Biosci Biotechnol Biochem 64, 2159–2164.CrossRefGoogle ScholarPubMed