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Comparison of the isotope dilution method for determination of the ileal endogenous amino acid losses with labelled diet and labelled pigs

Published online by Cambridge University Press:  09 March 2007

Vincent Hess ,
Philippe Ganier ,
Jean-Noel Thibault  and
Bernard Sève
Vincent Hess
Affiliation:
INRA, Station de Recherches Porcines, 35590 Saint Gilles, France
Philippe Ganier
Affiliation:
INRA, Station de Recherches Porcines, 35590 Saint Gilles, France
Jean-Noel Thibault
Affiliation:
INRA, Station de Recherches Porcines, 35590 Saint Gilles, France
Bernard Sève*
Affiliation:
INRA, Station de Recherches Porcines, 35590 Saint Gilles, France
*
*Corresponding author: Dr Bernard Sève, fax +33 2 99 28 50 80, email [email protected]
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Abstract

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The aims of the present study were first to compare the amino acid dilution method performed using labelled animals with that using labelled diets, and second to determine real digestibilities and total ileal endogenous losses of N and amino acids. Two diets containing pea cultivars (Solara and Amino) and a protein-free diet were compared in a 3 × 3 Latin-square experiment. The three pigs were each prepared with an ileo-rectal anastomosis and were continuously infused with [1-13C]leucine. For each cultivar, 15N-labelled and unlabelled diets were formulated. The real digestibility and endogenous losses of leucine were higher when obtained by labelling the pig than by labelling the foodstuff. This was due either to the inadequate estimation of the endogenous protein enrichment in the first case or to the importance of dietary N recycling in the second case. However, in both cases the ileal endogenous losses of N and amino acids were higher than the basal losses determined with the protein-free diet. There were significant differences between the two pea cultivars in terms of phenylalanine and leucine when measured with labelled diets. It is suggested that, although ileal endogenous losses may be underestimated, using labelled feedstuffs is of great interest due to the direct estimation of the individual amounts of amino acids.

Keywords

Amino acidsIsotope dilution methodEndogenous losses
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 2 , February 2000 , pp. 123 - 130
Copyright
Copyright © The Nutrition Society 2000

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