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Comparison of recoveries in breath carbon dioxide of H13CO-3 and H14CO-3 administered simultaneously by single 6 h constant unprimed intravenous infusion

Published online by Cambridge University Press:  09 March 2007

N. J. Fuller*
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
M. Harding
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
R. McDevitt
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
G. Jennings
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
W. A. Coward
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
M. Elia
Affiliation:
MRC Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
*
*Corresponding author: Dr N. Fuller, present address 3 Cherry Hinton Court, Cherry Hinton Road, Cambridge CB1 7AL, UK.
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Abstract

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The aim of this study was to assess the bioequivalence of H13CO-3 and H14CO-3, by administering both labels simultaneously by single infusion and comparing their recovery in breath CO2 and urinary urea. Six healthy male subjects (age range 24–41 years; weight 76·7 (SD, 18·6) KG; HEIGHT 1·79 (sd 0·05) m) were infused with unprimed solutions of HCO3- (110·0 mmol/kg) labelled with 13C (0·76 mmol 13C/h) and 14C (48 Bq/h) at a constant rate for 6 h, in a whole-body calorimeter (1400 litres) for measurement of CO2 production. Samples of breath were collected hourly in a Douglas bag and all urine was collected into two batches (0–4 h and 4–6 h) for estimating recovery of infused label by measurement of enrichment or specific activity. Recovery in breath CO2 of both labels increased from about 25% for the first hour to 88% and above for hours 3–4 onwards. Mean recovery of 13C in breath CO2 was slightly higher than that of 14C for all periods (mean difference always less than 1 % of infused label) but was significant only for the first 3h (P < 0·05). Recovery of 14C in urea was significantly higher (P < 0·01) than 13C, but was confounded by substantial variability and uncertainties concerning 13CO2 background enrichments. These results suggest that there is no compelling need to alter factors currently used for recovery of 14C in breath when using 13C instead, and vice versa.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2000

References

Beaufrere, B, Horber, FF, Schwenk, WF, Marsh, HM, Matthews, D, Gerich, JE and Haymond, MW (1989) Glucocorticosteroids increase leucine oxidation and impair leucine balance in humans. American Journal of Physiology 257, E712E721.Google ScholarPubMed
Craig, H (1957) Isotopic standards for carbon and oxygen and correction factors for mass-spectrometric analysis of carbon dioxide. Geochimica Cosmochimica Acta 12, 133149.CrossRefGoogle Scholar
Crisp, JA & Murgatroyd, PR (1985) In EURO-NUT Report 5, pp. 4445 [van Es, AJH, editor]. Wageningen: Agricultural University.Google Scholar
Elia, M, Fuller, NJ and Murgatroyd, PR (1992) Measurement of bicarbonate turnover in humans: applicability to estimation of energy expenditure. American Journal of Physiology 263, E676E687.Google ScholarPubMed
Elia, M, Jones, MG, Jennings, G, Poppitt, SD, Fuller, NJ, Murgatroyd, PR and Jebb, SA (1995) Estimating energy expenditure from specific activity of urine urea during lengthy subcutaneous NaH14CO3infusion. American Journal of Physiology 269, E172E182.Google Scholar
Elia, M and Livesey, G (1992) Energy expenditure and fuel selection in biological systems: the theory and practice of calculations based on indirect calorimetry and tracer methods. World Review of Nutrition and Dietetics 70, 68131.CrossRefGoogle ScholarPubMed
Farquhar, GD, Ehleringer, JR and Hubick, KT (1989) Carbon isotope discrimination and photosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology 40, 503537.CrossRefGoogle Scholar
Fuller, NJ and Elia, M (1989) Does mitochondrial compartmentation of CO2exist in man?. Clinical Physiology 9, 345352.CrossRefGoogle ScholarPubMed
Issekutz, B, Pavle, BP, Miller, HI and Bortz, WM (1968) Oxidation of plasma FFA in lean and obese humans. Metabolism 17, 6273.CrossRefGoogle ScholarPubMed
James, WPT, Garlick, PJ, Sender, PM and Waterlow, JC (1976) Studies of amino acids and protein metabolism in normal man with L-[U-13C] tyrosine. Clinical Science and Molecular Medicine 50, 525532.Google Scholar
Jones, PJH and Leatherdale, ST (1991) Stable isotopes in clinical research: safety reaffirmed. Clinical Science 80, 277280.CrossRefGoogle ScholarPubMed
Klein, PD (1991) Nutritional applications of 13C: strategic considerations. In New Techniques in Nutritional Research, pp. 7394 [Whitehead, RG & Prentice, A, editors]. London: Academic Press.Google Scholar
Leijssen, DPC and Elia, M (1996) Recovery of 13CO2 and 14CO2 in human bicarbonate studies: a critical review with original data. Clinical Science 91, 665677.CrossRefGoogle ScholarPubMed
Leijssen, DPC, Saris, WHM, Jeukendrup, AE and Wagenmakers, AJM (1995) Oxidation of exogenous [13C] galactose and [13C]glucose during exercise. Journal of Applied Physiology 79, 720725.CrossRefGoogle ScholarPubMed
O'Leary, MH (1981) Carbon isotope fractionation in plants. Phytochemistry 20, 553567.CrossRefGoogle Scholar
Pacy, PJ, Cheng, KN, Thompson, GN and Halliday, D (1989) Stable isotopes as tracers in clinical research. Annals of Nutrition and Metabolism 33, 6578.CrossRefGoogle ScholarPubMed
Saris, WHM, Goodpaster, BH, Jeukendrup, AE, Brouns, F, Halliday, D and Wagenmakers, AJM (1993) Exogenous carbohydrate oxidation from different carbohydrate sources during exercise. Journal of Applied Physiology 75, 21682172.CrossRefGoogle ScholarPubMed
Schoeller, DA, Klein, PD, Watkins, JB, Heim, T and MacClean, WC (1980) 13C abundances of nutrients and the effect of variations in C isotopic abundances of test meals formulated for 13CO2 breath tests. American Journal of Clinical Nutrition 33, 23752385.CrossRefGoogle Scholar
Schrauwen, P, van Aggel, , Leijssen, DPC, Lichtenbelt, WDV, van Baak, MA, Gijsen, AP and Wagenmakers, AJM (1998) Validation of the [1,2-13C]acetate recovery for correction of [U-13C]palmitate oxidation. Journal of Physiology 513, 215223.CrossRefGoogle ScholarPubMed
Sidossis, LS, Coggan, AR, Gastaldelli, A and Wolfe, RR (1995) A new correction factor for use in tracer estimations of plasma fatty acid oxidation. American Journal of Physiology 269, E649E656.Google ScholarPubMed
Spear, ML, Darmaun, D, Sager, BK, Parsons, WR and Haymond, MW (1995) Use of [13C]bicarbonate infusion for measurement of CO2 production. American Journal of Physiology 268, E1123E1127.Google ScholarPubMed
Wagenmakers, AJM, Rehrer, NJ, Brouns, F, Saris, WHM and Halliday, D (1993) Breath 13CO2 background enrichment during exercise: diet related differences between Europe and America. Journal of Applied Physiology 74, 23532357.CrossRefGoogle ScholarPubMed
Wolfe, RR and Jahoor, F (1990) Recovery of labeled CO2 during the infusion of C-1 vs C-2-labeled acetate: implications for tracer studies of substrate oxidation. American Journal of Clinical Nutrition 51, 248252.CrossRefGoogle ScholarPubMed