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Comparison of oral and intraperitoneal iron supplementation in anaemic rats: a re-evaluation of the mucosal block theory of iron absorption*

Published online by Cambridge University Press:  09 March 2007

Paloma Benito ,
William House  and
Dennis Miller
Paloma Benito
Affiliation:
Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
William House
Affiliation:
USDA ARS, US Plant, Soil and Nutrition Laboratory, Ithaca, NY 14853, USA
Dennis Miller
Affiliation:
Department of Food Science, Cornell University, Ithaca, NY 14853, USA
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Abstract

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To evaluate the extent to which daily oral Fe supplements may block Fe absorption from a subsequent dose, we compared effects of oral and intraperitoneal (i.p.) Fe supplementation on Fe status in anaemic rats (haemoglobin (Hb) 90 g/l). A ligated duodenal loop technique was used to assess the effects of the Fe supplements administered either orally or i.p. at different frequencies on Fe absorption from a subsequent test dose. Anaemic Sprague–Dawley rats were assigned to seven groups of eight rats each and received either oral or i.p. Fe supplements for 3 d as follows: (1) 4 mg oral supplement daily (three doses in 3 d); (2) 4 mg oral supplement once (one dose on day 1, low-Fe dose on days 2 and 3); (3) 12mg oral supplement once (one dose on day 1, low-Fe dose on days 2 and 3); (4) 3.2 mg i.p. supplement daily (three doses in 3d); (5) 3.2 mg i.p. supplement once (one dose on day 1); (6) 9.6 mg i.p. supplement once (one dose on day 1); (7) low-Fe diet (control). The effectiveness of the supplements in treating Fe deficiency on each of the two test-factors, i.e. route of administration and frequency of dose, was assessed by determining Hb-Fe gain and liver-Fe stores after the 3 d test period. Oral supplementation was as effective as i.p. in improving the Fe status of the anaemic animals. However, a 15 min absorption of a radio-Fe test dose from a ligated loop in i.p.-supplemented groups was significantly higher (11.68 (sd 1.70) %, 17.49 (sd 4.59) %, 16.71 (sd 3.39) %) than in orally supplemented groups (3.24 (sd 1.35) %, 2.45 (sd 1.05) %, 1.80 (sd 0.35) %) despite equal body Fe stores. No significant difference in intestinal Fe absorption efficiency was detected within the oral groups but those supplemented only once were more effective than or as effective as the group receiving daily supplements for 3d in improving Fe status as indicated by Hb-regeneration efficiency. We conclude that there is a mucosal block with the administration of oral Fe supplements but the extent of this blocking effect during oral Fe supplementation is not as dramatic as currently thought in the context of the poor efficacy of daily Fe supplementation programmes.

Keywords

IronAnaemiaIntestineMucosal block
Type
General Nutrition
Information
British Journal of Nutrition , Volume 79 , Issue 6 , June 1998 , pp. 533 - 540
Copyright
Copyright © The Nutrition Society 1998

References

Benito, P, House, W & Miller, D (1997) Influence of iron supplementation frequency on absorption efficiency and mucosal ferritin in anaemic rats. British Journal of Nutrition 78, 469–477.CrossRefGoogle ScholarPubMed
Bieri, JG (1980) Second report of the ad hoc committee on standards for nutritional studies. Journal of Nutrition 110, 1726.CrossRefGoogle Scholar
Bieri, JG, Stoewsand, GS, Briggs, GM, Woodard, JC & Knapka, JJ (1977) Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.Google Scholar
Bothwell, TH & Finch, CA (1962) Iron Metabolism. London, England: J & A Churchill Ltd.Google Scholar
Brittin, GM & Raval, D (1970) Duodenal ferritin synthesis during iron absorption in the iron-deficient rat. Journal of Laboratory and Clinical Medicine 75, 811–817.Google ScholarPubMed
Brittin, GM & Raval, D (1971) Duodenal ferritin synthesis in iron-replete and iron-deficient rats: Response to small doses of iron. Journal of Laboratory and Clinical Medicine 77, 54–58.Google Scholar
Brown, EB, Dubach, R & Moore, CV (1958) Studies in iron transportation and metabolism. XI. Critical analysis of mucosal block by large doses of inorganic iron in human subjects. Journal of Laboratory and Clinical Medicine 52, 335–355.Google ScholarPubMed
Conrad, ME & Crosby, WH (1963) Intestinal mucosal mechanisms controlling iron absorption. Blood 22, 406–415.CrossRefGoogle ScholarPubMed
Conrad, ME, Weintraub, LR & Crosby, WH (1964) The role of the intestine in iron kinetics. Journal of Clinical Investigation 43, 963–974.CrossRefGoogle ScholarPubMed
Cook, JD, Lipschitz, DA, Miles, LEM & Finch, CA (1974) Serum ferritin as a measure of iron stores in normal subjects. American Journal of Clinical Nutrition 27, 681–687.CrossRefGoogle ScholarPubMed
Crosby, WH (1963) The control of iron balance by the intestinal mucosa. Blood 22, 441–449.CrossRefGoogle ScholarPubMed
Drysdale, JW & Shafritz, DA (1975) Induction of ferritin by iron. In Proteins of Iron Storage and Transport in Biochemistry and Medicine, pp. 319–326 [Crichton, RR, editor]. Amsterdam, The Netherlands: North-Holland Publishing Company.Google Scholar
Granick, S (1946) Ferritin. IX. Increase of the protein apoferritin in the gastrointestinal mucosa as a direct response to iron feeding. The function of ferritin in the regulation of iron absorption. Journal of Biological Chemistry 164, 737–746.CrossRefGoogle Scholar
Hahn, PF, Bale, WF, Ross, JF, Balfour, WM & Whipple, GH (1943) Radioactive iron absorption by the gastrointestinal tract: Influence of anemia, anoxia, and antecedent feeding. Journal of Experimental Medicine 78, 169–188.CrossRefGoogle ScholarPubMed
Hahn, PF, Carothers, EL, Darby, WJ, Martin, M, Sheppard, CW, Cannon, RO, Beam, AS, Densen, PM, Peterson, JC & McClellan, GS (1951) Iron metabolism in human pregnancy as studied by radioactive isotope, 59Fe. American Journal of Obstetrics and Gynecology 61, 477–486.CrossRefGoogle Scholar
Levine, PH & Weintraub, LR (1970) Preparation of suspensions of small bowel mucosal epithelial cells. Journal of Laboratory and Clinical Medicine 75, 1026–1029.Google ScholarPubMed
McCurdy, PR (1965) Oral and parenteral iron therapy. Journal of the American Medical Association 191, 859–862.CrossRefGoogle ScholarPubMed
Momtazi, S & Herbert, V (1973) Intestinal absorption using vibration-obtained individual small bowel epithelial cells of the rat: Folate absorption. American Journal of Clinical Nutrition 26, 23–29.CrossRefGoogle ScholarPubMed
National Committee for, Clinical Laboratory Standards (1984). Reference Procedure for the Quantitative Determination of Hemoglobin in Blood; Approved Standard. Villanova, PA, USA: National Committee for Clinical Laboratory Standards.Google Scholar
Norrby, A & Sölvell, L (1974) Iron absorption and haemoglobin regeneration in posthaemorrhagic anaemia – Studies on the absorption pattern during oral iron therapy. Scandinavian Journal of Haematology 20, Suppl., 75–125.CrossRefGoogle Scholar
O'Neil-Cutting, MA & Crosby, WH (1987) Blocking of iron absorption by a preliminary oral dose of iron. Archives of Internal Medicine 147, 489–491.CrossRefGoogle Scholar
Pritchard, JA (1961) The response to iron in iron deficiency. Journal of the American Medical Association 175, 478–482.CrossRefGoogle ScholarPubMed
Savin, MA & Cook, JD (1978) Iron transport by isolated rat intestinal mucosal cells. Gastroenterology 75, 688–694.CrossRefGoogle ScholarPubMed
Schültink, W, Gross, R, Gliwitzki, M, Karyadi, D & Matulessi, P (1995) Effect of daily vs twice weekly iron supplementation in Indonesian preschool children with low iron status. American Journal of Clinical Nutrition 61, 111–115.CrossRefGoogle ScholarPubMed
Sch¨mann, K, Elsenhans, B, Ehtechami, C & Forth, W (1990) Increased intestinal iron absorption in rats with normal hepatic iron stores. Kinetic aspects of the adaptive response to parenteral iron repletion in dietary iron deficiency. Biochimica et Biophysica Acta 1033, 277–281.CrossRefGoogle Scholar
Snedecor, GW & Cochran, WG (1989) Statistical Methods. Ames, IA, USA: Iowa State University Press.Google Scholar
Solomons, NW, Pineda, O, Viteri, F & Sandstead, HH (1983) Studies on the bioavailability of zinc in humans: Mechanism of the intestinal interaction of nonheme iron and zinc. Journal of Nutrition 113, 337–349.CrossRefGoogle ScholarPubMed
Torrance, JD & Bothwell, TH (1968) A simple technique for measuring storage iron concentrations in formalinised liver samples. South African Journal of Medical Sciences 33, 9–11.Google ScholarPubMed
Viteri, FE (1996) Weekly compared with daily iron supplementation. American Journal of Clinical Nutrition 63, 610–614.CrossRefGoogle ScholarPubMed
Viteri, FE, Liu, X, Tolomei, K & Martin, A (1995) True absorption and retention of supplemental iron is more efficient when iron is administered every three days rather than daily to iron-normal and iron-deficient rats. Journal of Nutrition 125, 82–91.Google ScholarPubMed
Welch, RM & House, WA (1980) Absorption of radiocadmium and radioselenium by rats fed intrinsically and extrinsically labeled lettuce leaves. Nutrition Reports International 21, 135–145.Google Scholar
Wheby, MS & Crosby, WH (1963) The gastrointestinal tract and iron absorption. Blood 22, 416–428.CrossRefGoogle ScholarPubMed
Whittaker, P, Skikne, BS, Covell, AM, Flowers, C, Cooke, A, Lynch, SR & Cook, JD (1989) Duodenal iron proteins in idiopathic hemochromatosis. Journal of Clinical Investigation 83, 261–267.CrossRefGoogle ScholarPubMed
Wood, JK, Milner, PFA & Pathak, UN (1968) The metabolism of iron-dextran given as a total-dose infusion to iron deficient Jamaican subjects. British Journal of Haematology 14, 119–129.CrossRefGoogle Scholar
Wright, AJA & Southon, S (1990) The effectiveness of various iron-supplementation regimens in improving the Fe status of anaemic rats. British Journal of Nutrition 63, 579–585.CrossRefGoogle ScholarPubMed