Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-23T19:56:13.888Z Has data issue: false hasContentIssue false
  • English
  • Français

Changes in insulin-receptor mRNA levels in skeletal muscle and brown adipose tissue of weanling rats during fasting and refeeding

Published online by Cambridge University Press:  09 March 2007

Rachel M. Knott ,
Paul Trayhurn  and
John E. Hesketh
Rachel M. Knott
Affiliation:
Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Paul Trayhurn
Affiliation:
Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
John E. Hesketh
Affiliation:
Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Tissue-specific alterations in insulin sensitivity occur in response to fasting and refeeding, as part of the integrated adaptive mechanisms employed to adjust to major changes in nutritional status. In the present study the effects of fasting and refeeding on insulin-receptor, actin and myosin mRNA levels in skeletal muscle, and insulin-receptor and uncoupling-protein mRNA in brown adipose tissue of rats have been examined. Insulin-receptor mRNA levels increased markedly in both skeletal muscle and brown adipose tissue after a 40 h fast, the increase being greater in brown fat (8-fold) than in muscle (2-fold). On refeeding for 4 h, the insulin-receptor mRNA level in both tissues declined rapidly to control levels. An increase in insulin-receptor mRNA level was also observed in brown adipose tissue after a 16 h fast, although not in skeletal muscle. In contrast to the insulin-receptor mRNA, the level of the mRNA for the mitochondrial uncoupling protein declined markedly in brown adipose tissue during a 40 h fast. These results indicate that insulin-receptor mRNA levels are modulated in response to the alterations in nutritional status that occur during fasting and refeeding; this may reflect a nutritional influence on transcription of the receptor-protein gene

Keywords

Insulin-receptor mRNASkeletal muscleBrown adipose tissue
Type
Metabolic Effects of Feeding
Information
British Journal of Nutrition , Volume 68 , Issue 3 , November 1992 , pp. 583 - 592
Copyright
Copyright © The Nutrition Society 1992

References

Balage, M., Sornet, C., Manin, M. & Grizard, J. (1989). Insulin receptor binding and tyrosine kinase activity in liver and skeletal muscle from fasted rats. Reproduction, Nutrition and Development 29, 685–687.Google Scholar
Brady, L. J., Goodman, M. N., Kalish, F. N. & Ruderman, N. B. (1981). Insulin binding and sensitivity in rat skeletal muscle: effect of starvation. American Journal of Physiology 240, E184–E190.Google Scholar
Briata, P., Briata, L. & Gherzi, R. (1990). Glucose starvation and glycosylation inhibitors reduce insulin receptor gene expression: characterization and potential mechanism in human cells. Biochemical and Biophysical Research Communications 169, 397–405.CrossRefGoogle ScholarPubMed
Burnol, A-F., Ebner, S., Kande, J. & Girard, J. (1990). Insulin resistance of glucose metabolism in isolated brown adipocytes of lactating rats. Biochemical Journal 265, 511–517.CrossRefGoogle ScholarPubMed
Champigny, O. & Ricquier, D. (1990). Effect of fasting and refeeding on uncoupling protein-mRNA levels in rat brown adipose tissue. Journal of Nutrition 120, 1730–1736.Google Scholar
Charron, M. J. & Kahn, B. B. (1990). Divergent mechanisms for insulin resistant glucose transport in muscle and adipose cells in vivo. Journal of Biological Chemistry 265, 7994–8000.Google Scholar
Chomczynski, P. & Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162, 156–159.CrossRefGoogle ScholarPubMed
Contreras, I., Dohm, G. L., Abdallah, S., Wells, J. A., Mooney, N., Rovira, A. & Caro, J. F. (1990). The effect of fasting on the activation in vivo of the insulin receptor kinase. Biochemical Journal 265, 887–890.Google Scholar
Crettaz, M. & Jeanrenaud, B. (1980). Post receptor alterations in the state of insulin resistance. Metabolism 29, 467–473.CrossRefGoogle Scholar
Crettaz, M., Prentki, M., Zaninetti, D. & Jeanrenaud, B. (1980). Insulin resistance in soleus muscle from obese Zucker rats: involvement of several defective sites. Biochemical Journal 186, 525–534.CrossRefGoogle ScholarPubMed
Ebina, Y., Ellis, L., Jarnagin, M., Edery, M., Graf, L., Clauser, E., Ou, J.-H., Masiarz, F., Kan, Y. W., Goldfine, I. D., Roth, R. A. & Rutter, W. J. (1985). The human insulin receptor cDNA: the structural basis for hormone activated transmembrane signalling. Cell 40, 747–758.Google Scholar
Erickson, J. M., Rushford, C. L., Dorney, D. J., Wilson, G. N. & Schmickel, R. D. (1985). Structure and variation of human ribosomal DNA: molecular analysis of cloned fragments. Gene 16, 1–9.Google Scholar
Ferré, P., Burnol, A.-F., Leturque, A., Terretaz, J., Penicaud, L., Jeanrenaud, B. & Girard, J. (1986). Glucose utilization in vivo and insulin sensitivity of rat brown adipose tissue in various physiological and pathological conditions. Biochemical Journal 233, 249–252.Google Scholar
Gavin, J. R., Roth, J., Neville, D. M., De Meyts, P. & Buell, D. N. (1974). Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proceedings of the National Academy of Sciences U.S.A. 71, 84–88.CrossRefGoogle ScholarPubMed
Goldstein, B. J. & Kahn, C. R. (1989). Analysis of mRNA heterogeneity by ribonuclease H mapping: application to the insulin receptor. Biochemical and Biophysical Research Communications 159, 664–669.CrossRefGoogle ScholarPubMed
Goldstein, B. J., Muller-Wieland, D. & Kahn, C. R. (1987). Variation in insulin receptor messenger ribonucleic acid expression in human and rodent tissues. Molecular Endocrinology 1, 759–766.CrossRefGoogle ScholarPubMed
Gould, G. W. & Bell, G. I. (1990). Facilitative glucose transporters: an expanding family. Trends in Biochemical Sciences 15, 18–23.Google Scholar
Grigorescu, F., Flier, J. S. & Kahn, C. R. (1984). Defect in insulin receptor phosphorylation in erythrocytes and fibroblasts associated with severe insulin resistance. Journal of Biological Chemistry 259, 15003–15006.CrossRefGoogle ScholarPubMed
Isaad, T., Penicaud, L., Ferré, P., Kande, J., Baudon, M.-A. & Girard, J. (1987). Effects of fasting on tissue glucose utilization in conscious resting rats. Biochemical Journal 246, 241–244.Google Scholar
Kadowaki, T., Kasuga, M., Akanuma, Y., Ezaki, O. & Takaku, F. (1984). Decreased autophosphorylation of the insulin receptor kinase in streptozotocin-diabetic rats. Journal of Biological Chemistry 259, 14208–14216.Google Scholar
Kahn, B. B., Simpson, I. A. & Cushman, S. W. (1988). Divergent mechanisms for the insulin resistant and hyper-responsive glucose transport in adipose cells from fasted and re-fed rats. Journal of Clinical Investigation 82, 691–699.CrossRefGoogle Scholar
Knott, R. M., Hesketh, J. E. & Trayhurn, P. (1990) Insulin receptor gene expression in skeletal muscle and brown adipose tissue. Biochemical Society Transactions 18, 1263.CrossRefGoogle ScholarPubMed
Levy, J. R., Krystal, G., Glickman, P. & Dastvan, F. (1991). Effects of media conditions, insulin and dexamethasone on insulin-receptor mRNA and promoter activity in HepG2 cells. Diabetes 40, 58–65.Google Scholar
MacFarlane, F. & Trayhurn, P. (1990). Oligonucleotide probe for the cross-species measurement of the mRNA for uncoupling protein in brown adipose tissue. Biochemical Society Transactions 18, 1261.Google Scholar
Mercer, S. W. & Trayhurn, P. (1984). The development of insulin resistance in brown adipose tissue may impair the acute cold-induced activation of thermogenesis in genetically obese (ob/ob) mice. Bioscience Reports 4, 933–940.CrossRefGoogle ScholarPubMed
Nielson, D. A. & Shapiro, D. J. (1990). Insights into hormonal control of mRNA stability. Molecular Endocrinology 4, 953–957.Google Scholar
Penicaud, L., Kande, J., Le Magnen, J. & Girard, J. (1985). Insulin action during fasting and refeeding in rat, determined by euglycaemic clamp. American Journal of Physiology 249, E514–E518.Google Scholar
Ricquier, D., Bouillaud, F., Toumelin, P., Mory, G., Bazin, R., Arch, J. & Penicaud, L. (1986). Expression of uncoupling protein mRNA in thermogenic or weakly thermogenic brown adipose tissue. Journal of Biological Chemistry 261, 13905–13910.Google Scholar
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. 2nd ed. Cold Spring Harbor N. Y.: Cold Spring Harbor Laboratory Press.Google Scholar
Trayhurn, P. & Jennings, G. (1988). Non-shivering thermogenesis and the thermogenic capacity of brown fat in fasted/refed mice. American Journal of Physiology 254, R11–R16.Google Scholar
Trinder, P. (1969). Determination of glucose in blood using glucose oxidase with an alternate oxygen acceptor. Analytical Clinical Biochemistry 6, 24–27.Google Scholar
Ullrich, A., Bell, J. R., Chen, E. Y., Herrera, R., Petruzzelli, L. M., Dull, T. J., Gray, A., Coussens, L., Liao, Y.-C., Tsubokawa, M., Mason, A., Seeburg, P. H., Grunfeld, C., Rosen, O M. & Ramachandran, J. (1985). Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313, 756–761.Google Scholar