Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-23T03:55:43.222Z Has data issue: false hasContentIssue false
  • English
  • Français

Bone status in an animal model of chronic sub-optimal nutrition: a morphometric, densitometric and mechanical study

Published online by Cambridge University Press:  08 March 2007

Patricia M. Boyer ,
Gabriela E. Compagnucci ,
María I. Olivera ,
Clarisa Bozzini ,
María C. Roig ,
Cecilia V. Compagnucci  and
Rosa M. Alippi
Patricia M. Boyer*
Affiliation:
Department of Physiology, School of Dentistry, University of Buenos Aires, Marcelo T. de Alvear 2142 (C1122 AAH), Buenos Aires, Argentina
Gabriela E. Compagnucci
Affiliation:
Department of Physiology, School of Dentistry, University of Buenos Aires, Marcelo T. de Alvear 2142 (C1122 AAH), Buenos Aires, Argentina
María I. Olivera
Affiliation:
Department of Physiology, School of Dentistry, University of Buenos Aires, Marcelo T. de Alvear 2142 (C1122 AAH), Buenos Aires, Argentina
Clarisa Bozzini
Affiliation:
Department of Physiology, School of Dentistry, University of Buenos Aires, Marcelo T. de Alvear 2142 (C1122 AAH), Buenos Aires, Argentina
María C. Roig
Affiliation:
Department of Physiology, School of Dentistry, University of Buenos Aires, Marcelo T. de Alvear 2142 (C1122 AAH), Buenos Aires, Argentina
Cecilia V. Compagnucci
Affiliation:
Department of Physiology, School of Dentistry, University of Buenos Aires, Marcelo T. de Alvear 2142 (C1122 AAH), Buenos Aires, Argentina
Rosa M. Alippi
Affiliation:
Department of Physiology, School of Dentistry, University of Buenos Aires, Marcelo T. de Alvear 2142 (C1122 AAH), Buenos Aires, Argentina
*
*Corresponding author: Dr Patricia M. Boyer, fax +5411 4508 3958, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In children, inappropriate eating habits can induce a disease known as nutritional dwarfing (ND). Due to the link between nutritional condition and bone growth, the effects induced by a 20 % reduction of food intake on bone competence were assessed in an animal model of ND. Bone status during catch-up growth was also analysed. Male Wistar rats were divided into control (C) and ND groups. C rats were fed ad libitum. ND received 80 % of the diet consumed by C for 4 weeks (T4); thereafter, they were fed ad libitum for 8 weeks. Results, expressed as mean (sem) for ND v. C, were as follows. At T4, body weight (g) and length (cm) and femur weight (g) and length (mm) were 97·35 (sem 5·89) v. 199·07 (sem 9·24), 16·91 (sem 0·41) v. 20·26 (sem 0·31), 0·30 (sem 0·01) v. 0·46 (sem 0·01) and 23·09 (sem 0·29) v. 26·98 (sem 0·26), respectively (P<0·001); bone mineral content (g) and density (g/cm2) were 0·014 (sem 0·002) v. 0·030 (sem 0·002) and 0·061 (sem 0·004) v. 0·080 (sem 0·003), respectively (P<0·001); load-bearing capacity (N), yielding load (N) and elastic stiffness (N/mm) were 25·06 (sem 1·24) v. 50·34 (sem 2·94), 23·72 (sem 1·02) v. 46·97 (sem 1·75) and 65·98 (sem 4·42) v. 115·07 (sem 3·85), respectively (P<0·001); cross-sectional area (mm2) and moment of inertia (mm4) were 2·86 (sem 0·19) v. 4·54 (sem 0·17) and 1·27 (sem 0·08) v. 3·03 (sem 0·16), respectively (P<0·001). Significant effects were not evident in material properties. Parameters assessed normalized during re-feeding. These results suggest that the impaired mechanical femur competence in ND rats could be due to an altered bone mass and architectural distribution rather than to intrinsic quality. Re-feeding caused a reversal of the effects of food restriction on growth and bone parameters in ND rats.

Keywords

Weanling ratFood restrictionDensitometryMechanical performance
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 5 , May 2005 , pp. 663 - 669
Copyright
Copyright © The Nutrition Society 2005

References

Akeson, PK, Axelsson, IEM, Raiha, NCR, Warm, A, Minoli, I & Moro, G (2000) Fat intake and metabolism in Swedish and Italian infants. Acta Paediatr 89, 28–33.CrossRefGoogle ScholarPubMed
Ammann, P, Rizzoli, R, Sloman, D & Bonjour, JP (1992) Sequential and precise in vivo measurement of bone mineral density in rats using dual-energy X-ray absorptiometry. J Bone Miner Res 7, 311–316.CrossRefGoogle ScholarPubMed
Boersma, B & Wit, JM (1997) Catch-up growth. Endocr Rev 18, 646–661.CrossRefGoogle ScholarPubMed
Bonjour, JP, Ammann, P, Chevalley, T & Rizzoli, R (2001) Protein intake and bone growth. Can J Appl Physiol 26, 153–166.CrossRefGoogle ScholarPubMed
Bourrin, S, Ammann, P, Bonjour, JP & Rizzoli, R (2000) Dietary protein restriction lowers plasma insulin-like growth factor I (IGF-I), impairs cortical bone formation, and induces osteoblastic resistance to IGF-I in adult female rats. Endocrinology 141, 3149–3155.CrossRefGoogle ScholarPubMed
Cameron, JL, Helmreich, DL & Schreihofer, DA (1993) Modulation of reproductive hormone secretion by nutritional intake: stress signals versus metabolic signals. Hum Reprod 8, 162–167.CrossRefGoogle ScholarPubMed
Compagnucci, CV, Compagnucci, GE, Lomniczi, A, Mohn, C, Vacas, I, Cebral, E, Elverdin, J, Friedman, S, Rettori, V, Boyer, P (2002–2003) Effect of nutritional stress on the hypothalamo–pituitary–gonadal axis in the growing male rats Neuroimmunomodulation 10, 153–162.CrossRefGoogle Scholar
Cummings, SR, Kelsey, JL, Nevitt, MC, O'Dowd, KJ (1985) Epidemiology of osteoporosis and osteoporotic fractures. Epidemiol Rev 7, 178–208.CrossRefGoogle ScholarPubMed
Eaton-Evans, J (1994) Osteoporosis and the role of diet. Br J Biomed Sci 51, 358–370.Google ScholarPubMed
Ferretti, JL (1997) Biomechanical properties of bone. In Osteoporosis and Bone Densitometry, pp. 143–161 [Gennant, HK, Guglielmi, G, Jergas, M, editors]. Berlin: Springer Verlag.Google Scholar
Ferretti, JL, Capozza, R, Cointry, G, Bozzini, C, Alippi, RM & Bozzini, CE (1991) Additive effects of dietary protein and energy deficiencies on diaphysis and bone tissue of rat femurs as determined by bending tests. Acta Physiol Pharmacol Ther Latinoam 41, 253–262.Google ScholarPubMed
Ferretti, JL, Tessaro, RD, Delgado, CJ, Bozzini, CE, Alippi, RM, Barceló, AC (1988) Biomechanical performance of diaphyseal shafts and bone tissue of femurs from protein-restricted rats. Bone Miner 4, 329–339.Google ScholarPubMed
Friedman, SM, Boyer, PM, Barrio, Rendo, ME, Morasso, MC, Gamba C, Río, ME (1999) Evaluación del crecimiento normal en ratas a través del puntaje Z. Arch Latinoam Nutr 49, 143–148.Google ScholarPubMed
Friedman, SM, Rodriguez, PN, Olivera, MI, Bozzini, C, Norese, F, Gamba, CA & Boyer, PM (1998) Enanismo por desnutrición: cronodinamia de los procesos metabólicos en ratas. Medicina (B Aires) 58, 282–286.Google Scholar
Galler, JR & Propert, KJ (1981) The effect of protein deficiency on weight gain and body composition in the developing rat. Nutr Rep Int 24, 885–892.Google Scholar
Griffin, MG, Kimble, R, Hopper, W & Pacifici, R (1993) Dual-energy X-ray absorptiometry of the rat: accuracy, precision and measurement of bone loss. J Bone Miner Res 7, 795–800.CrossRefGoogle Scholar
Hassager, C & Christiansen, C (1995) Measurement of bone mineral density. Calcif Tissue Int 57, 1–5.CrossRefGoogle ScholarPubMed
Javaid, MK & Cooper, C (2002) Prenatal and childhood influences on osteoporosis. Best Pract Res Clin Endocrinol Metab 16, 349–367.CrossRefGoogle ScholarPubMed
Kaplan, RM & Toshima, MT (1992) Does a reduced fat diet cause retardation in child growth?. Prev Med 21, 33–52.CrossRefGoogle ScholarPubMed
Keller, W & Fillmore, CM (1983) Prevalence of protein–energy malnutrition. World Health Stat Q 36, 129–167.Google ScholarPubMed
Lane, MA, Reznick, AZ, Tilmont, EM, Lanir, A, Ball, SS, Read, V, Ingram, DK, Cutler, RG & Roth, GS (1995) Aging and food restriction alter some indices of bone metabolism in male rhesus monkeys ( Macaca mulatta ). J Nutr 125, 1600–1610.Google ScholarPubMed
Lee, CJ, Panemanglore, M & Wilson, K (1986) Effect of dietary energy restriction on bone mineral content of mature rats. Nutr Res 6, 51–59.CrossRefGoogle Scholar
Leonard, MB & Zemel, BS (2002) Current concepts in pediatric bone disease. Pediatr Clin North Am 49, 143–173.CrossRefGoogle ScholarPubMed
Lifshitz, F & Moses, N (1988) Nutritional dwarfing: growth, dieting and fear of obesity. J Am Coll Nutr 7, 367–376.CrossRefGoogle ScholarPubMed
Lifshitz, F & Moses, N (1989) Growth failure: a complication of dietary treatment of hypercholesterolemia. Am J Dis Child 143, 537–542.CrossRefGoogle ScholarPubMed
Lifshitz, F, Moses, N, Cervantes, C & Ginsberg, L (1987) Nutritional dwarfing in adolescents. Semin Adolesc Med 3, 255–266.Google ScholarPubMed
Ndiaye, B, Cournot, G, Pelissier, M, Debray, OW & Lemonnier, D (1995a) Rat serum osteocalcin is decreased by restriction of energy intake. J Nutr 125, 1283–1290.Google ScholarPubMed
Ndiaye, B, Lemonnier, D, Sall, MG, Prudhon, C, Diaham, B, Zeghoud, F, Guillozo, H, Leite, N & Wade, S (1995b) Serum osteocalcin regulation in protein–energy malnourished children. Pediatr Res 37, 606–610.CrossRefGoogle ScholarPubMed
Nguyen, TV, Sambrook, PN & Eisman, JA (1998) Bone loss, physical activity, and weight change in elderly woman: The Dubbo Osteoporosis Epidemiology Study. J Bone Miner Res 13, 1458–1467.CrossRefGoogle ScholarPubMed
Paniagua, JG, Díaz-Curiel, M, De la, Piedra, Gordo, C, Castilla, Reparaz, C, Torralbo, García, M (1998) Bone mass assessment in rats by dual energy X-ray absorptiometry. Br J Radiol 71, 754–758.CrossRefGoogle Scholar
Pugliese, MT, Weyman-Daum, M, Moses, N & Lifshitz, F (1987) Parental health beliefs as a cause of nonorganic failure to thrive. Pediatrics 80, 175–182.CrossRefGoogle ScholarPubMed
Rhee, Y, Namgung, R, Park, DH, Lee, HC, Huh, GB & Lim, SK (2002) The effects of recombinant human parathyroid hormone, rhPTH(1-84), on bone mass in undernourished rats. J Endocrinol 174, 419–425.CrossRefGoogle Scholar
Sandberg, DE, Smith, MM, Fornari, V, Goldstein, M & Lifshitz, F (1991) Nutritional dwarfing: is it a consequence of disturbed psychosocial functioning?. Pediatrics 88, 926–933.CrossRefGoogle ScholarPubMed
Slosman, DO, Casez, JP, Pichard, C, Rochat, T, Fery, F, Rizzoli, R, Bonjour, JP, Morabia, A & Donath, A (1992) Assessment of whole-body composition with dual-energy X-ray absortiometry. Radiology 185, 593–598.CrossRefGoogle Scholar
Sokal, R & Rohlf, J (1994) Biometry: The Principles and Practice of Statistics in Biological Research. San Francisco, CA: WH Freeman & Co.Google Scholar
Talbott, SM, Cifuentes, M, Dunn, M & Shapses, S (2001) Energy restriction reduces bone density and biomechanical properties in aged female rats. J Nutr 131, 2382–2387.CrossRefGoogle ScholarPubMed
Talbott, SM, Rothkopf, MM & Shapses, S (1998) Dietary restriction of energy and calcium alters bone turnover and density in younger and older female rats. J Nutr 128, 640–645.CrossRefGoogle ScholarPubMed
Turner, CH & Burr, DB (1993) Basic biomechanical measurements of bone: a tutorial. Bone 14, 595–608.CrossRefGoogle Scholar