Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T05:06:25.608Z Has data issue: false hasContentIssue false
  • English
  • Français

The bioavailability and postprandial utilisation of sweet lupin (Lupinus albus)-flour protein is similar to that of purified soyabean protein in human subjects: a study using intrinsically 15N-labelled proteins

Published online by Cambridge University Press:  09 March 2007

François Mariotti ,
Maria E. Pueyo ,
Daniel Tomé  and
Sylvain Mahé
Maria E. Pueyo
Affiliation:
UMR INRA-INAPG de Physiologie de la Nutrition et du Comportement Alimentaire, Institut, National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris cedex 05, France
Daniel Tomé
Affiliation:
UMR INRA-INAPG de Physiologie de la Nutrition et du Comportement Alimentaire, Institut, National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris cedex 05, France
Sylvain Mahé
Affiliation:
UMR INRA-INAPG de Physiologie de la Nutrition et du Comportement Alimentaire, Institut, National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris cedex 05, France
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Sweet lupin (Lupinus albus), a protein-rich legume devoid of anti-nutritional factors, is considered to have a high potential for protein nutrition in man. Results concerning the nutritional value of lupin protein are, however, conflicting in animals and very scarce in human subjects. Furthermore, where fibre-rich protein sources are concerned, the long-term nutritional results are often obscured, particularly since fibre-promoted colonic fermentation may bias the energy supply and redistribute N flux. We therefore studied, during the postprandial phase, the bioavailability and utilisation of lupin-flour protein in nine healthy men who had ingested a mixed meal containing intrinsically 15N-labelled lupin flour as the protein source (Expt 1). The real ileal digestibility (RID) and ileal endogenous N losses (IENL) were assessed using a perfusion technique at the terminal ileum, and the N content and 15N enrichment of ileal samples. Lupin flour exhibited a high RID of 91 (SD 3) % AND LOW IENL (5·4 (sd 1·3) mmol N/h). Postprandial dietary deamination was also assessed from body dietary urea and urinary dietary N excretion, and compared with results in nine healthy men following an isoenergetic meal containing a 15N-soyabean-protein isolate with a similar RID, as a control (Expt 2). Postprandial dietary deamination was similar after lupin and soyabean meals (17 (sd 2) and 18 (sd 4) % ingested N respectively). We therefore conclude that lupin protein is highly bioavailable, even if included in fibre-rich flour, and that it can be used with the same efficiency as soyabean protein to achieve postprandial protein gain in healthy human subjects.

Keywords

Soyabean proteinLupin proteinIleal digestibilityPostprandial retentionNitrogen isotopes
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 4 , April 2002 , pp. 315 - 323
Copyright
Copyright © The Nutrition Society 2002

References

Ballester, DR, Brunser, O, Saitua, MT, Egana, JI, Yanez, EO & Owen, DF (1984) Safety evaluation of sweet lupine (Lupinus albus cv. Multolupa). II. Nine-month feeding and multigeneration study in rats. Food and Chemical Toxicology 22, 45–48.CrossRefGoogle ScholarPubMed
Batterham, ES (1992) Availability and utilization of amino acids for growing pigs. Nutrition Research Reviews 5, 1–18.CrossRefGoogle ScholarPubMed
Corring, T, Juste, C & Lhoste, E (1989) Nutritional regulation of pancreatic and biliary secretions. Nutrition Research Reviews 2, 161–180.CrossRefGoogle ScholarPubMed
Dunaif, G & Schneeman, BO (1981) The effect of dietary fiber on human pancreatic enzyme activity in vitro. American Journal of Clinical Nutrition 34, 1034–1035.CrossRefGoogle ScholarPubMed
Egana, JI, Uauy, R, Cassorla, X, Barrera, G & Yanez, E (1992) Sweet lupin protein quality in young men. Journal of Nutrition 122, 2341–2347.CrossRefGoogle ScholarPubMed
Eggum, BO, Tomes, G, Beames, RM & Datta, FU (1993) Protein and energy evaluation with rats of seed from 11 lupin cultivars. Animal Feed Science and Technology 43, 109–119.CrossRefGoogle Scholar
El-Khoury, AE, Pereira, PC, Borgonha, S, Basile-Filho, A, Beaumier, L, Wang, SY, Metges, CC, Ajami, AM & Young, VR (2000) Twenty-four-hour oral tracer studies with L-[1-13C]lysine at a low (15 mg.kg-1.d-1) and intermediate (29 mg.kg-1.d-1) lysine intake in healthy adults. American Journal of Clinical Nutrition 72, 122–130.CrossRefGoogle Scholar
Fernandez, JA & Batterham, ES (1995) The nutritive value of lupin-seed and dehulled lupin-seed meals as protein sources for growing pigs as evaluated by different techniques. Animal Feed Science and Technology 53, 279–296.CrossRefGoogle Scholar
Food and Agriculture Organization/World Health Organization (1990) Protein quality evaluation. In Joint FAO/WHO Expert Consultation. Rome: FAO/WHO.Google Scholar
Forsum, E, Goranzon, H & Thilen, M (1982) Protein evaluation of mixed diets in young adults, growing pigs, and growing rats. American Journal of Clinical Nutrition 36, 505–513.CrossRefGoogle ScholarPubMed
Fuller, MF & Garlick, PJ (1994) Human amino acid requirements: can the controversy be resolved? Annual Review of Nutrition 14, 217–241.CrossRefGoogle ScholarPubMed
Garlick, PJ, Clugston, GA, Swick, RW & Waterlow, JC (1980) Diurnal pattern of protein and energy metabolism in man. American Journal of Clinical Nutrition 33, 1983–1986.CrossRefGoogle ScholarPubMed
Gausserès, N, Mahé, S, Benamouzig, R, Luengo, C, Drouet, H, Rautureau, J & Tomé, D (1996) The gastro-ileal digestion of 15N-labelled pea nitrogen in adult humans. British Journal of Nutrition 76, 75–85.CrossRefGoogle ScholarPubMed
Gdala, J, Jansman, AJM, van Leeuwen, P, Huisman, J & Verstegen, MWA (1996) Lupins (L. luteus, L. albus, L. angustifolius) as a protein source for young pigs. Animal Feed Science and Technology 62, 239–249.CrossRefGoogle Scholar
Grala, W, Verstegen, MW, Jansman, AJ, Huisman, J & van Leeusen, P (1998) Ileal apparent protein and amino acid digestibilities and endogenous nitrogen losses in pigs fed soybean and rapeseed products. Journal of Animal Science 76, 557–568.CrossRefGoogle ScholarPubMed
Grant, G, Dorward, PM, Buchan, WC, Armour, JC & Pusztai, A (1995) Consumption of diets containing raw soyabeans (Glycine max), kidney beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata) or lupin seeds (Lupinus angustifolius) by rats for up to 700 days: effects on body composition and organ weights. British Journal of Nutrition 73, 17–29.CrossRefGoogle ScholarPubMed
Huyghe, C (1997) White lupin (Lupinus albus L.). Field Crops Research 53, 147–160.CrossRefGoogle Scholar
Isaksson, G, Lilja, P, Lundquist, I & Ihse, I (1983) Influence of dietary fiber on exocrine pancreatic function in the rat. Digestion 27, 57–62.CrossRefGoogle ScholarPubMed
Lenis, NP, Bikker, P, van der Meulen, J, van Diepen, JT, Bakker, JG & Jongbloed, AW (1996) Effect of dietary neutral detergent fiber on ileal digestibility and portal flux of nitrogen and amino acids and on nitrogen utilization in growing pigs. Journal of Animal Science 74, 2687–2699.CrossRefGoogle ScholarPubMed
Lien, KA, McBurney, MI, Beyde, BI, Thomson, AB & Sauer, WC (1996) Ileal recovery of nutrients and mucin in humans fed total enteral formulas supplemented with soy fiber. American Journal of Clinical Nutrition 63, 584–595.CrossRefGoogle ScholarPubMed
McNiven, MA & Castell, AG (1995) Replacement of soybean meal with lupinseed (Lupinus albus) in pigs starter diets. Animal Feed Science and Technology 52, 333–338.CrossRefGoogle Scholar
Mahé, S, Huneau, JF, Marteau, P, Thuillier, F & Tomé, D (1992) Gastroileal nitrogen and electrolyte movements after bovine milk ingestion in humans. American Journal of Clinical Nutrition 56, 410–416.CrossRefGoogle ScholarPubMed
Mahé, S, Roos, N, Benamouzig, R, Davin, L, Luengo, C, Gagnon, L, Gausserès, N, Rautureau, J & Tomé, D (1996) Gastrojejunal kinetics and the digestion of [15N]beta-lactoglobulin and casein in humans: the influence of the nature and quantity of the protein. American Journal of Clinical Nutrition 63, 546–552.CrossRefGoogle ScholarPubMed
Mariotti, F, Mahé, S, Luengo, C, Benamouzig, R & Tomé, D (2000) Postprandial modulation of dietary and whole-body nitrogen utilization by carbohydrates in humans. American Journal of Clinical Nutrition 72, 954–962.CrossRefGoogle ScholarPubMed
Mariotti, F, Pueyo, ME, Tomé, D, Bérot, S, Benamouzig, R & Mahé, S (2001) The influence of the albumin fraction on the bioavail-ability and postprandial utilization of pea protein given selectively to humans. Journal of Nutrition 131, 1706–1713.CrossRefGoogle Scholar
Medhus, AW, Sandstad, O, Bredesen, J & Husebye, E (1999) Delay of gastric emptying by duodenal intubation: sensitive measurement of gastric emptying by the paracetamol absorption test. Alimentary Pharmacology and Therapeutics 13, 609–620.CrossRefGoogle ScholarPubMed
Millward, DJ (1999) The nutritional value of plant-based diets in relation to human amino acid and protein requirements. Proceedings of the Nutrition Society 58, 249–260.CrossRefGoogle ScholarPubMed
Millward, DJ, Fereday, A, Gibson, NR & Pacy, PJ (2000) Human adult amino acid requirements: [1-13C]leucine balance evaluation of the efficiency of utilization and apparent requirements for wheat protein and lysine compared with those for milk protein in healthy adults. American Journal of Clinical Nutrition 72, 112–121.CrossRefGoogle ScholarPubMed
Millward, DJ & Pacy, PJ (1995) Postprandial protein utilization and protein quality assessment in man (editorial). Clinical Science (Colch) 88, 597–606.CrossRefGoogle Scholar
Molvig, L, Tabe, LM, Eggum, BO, Moore, AE, Craig, S, Spencer, D & Higgins, TJ (1997) Enhanced methionine levels and increased nutritive value of seeds of transgenic lupins (Lupinus angustifolius L.) expressing a sunflower seed albumin gene. Proceedings of the National Academy of Sciences, USA 94, 8393–8398.CrossRefGoogle ScholarPubMed
Mossé, J (1990) Nitrogen to protein conversion factor for ten cereals and six legumes or oilseeds. A reappraisal of its definition and determination. Variation according to species and to seed protein content. Journal of Agricultural and Food Chemistry 38, 18–24.CrossRefGoogle Scholar
Muir, HE, Murray, SM, Fahey, GC Jr, Merchen, NR & Reinhart, GA (1996) Nutrient digestion by ileal cannulated dogs as affected by dietary fibers with various fermentation characteristics. Journal of Animal Science 74, 1641–1648.CrossRefGoogle ScholarPubMed
Muller-Lissner, SA, Fimmel, CJ, Will, N, Muller-Duysing, W, Heinzel, F & Blum, AL (1982) Effect of gastric and transpyloric tubes on gastric emptying and duodenogastric reflux. Gastroenterology 83, 1276–1279.CrossRefGoogle ScholarPubMed
Nyachoti, CM, de Lange, CF, McBride, BW, Leeson, S & Gabert, VM (2000) Endogenous gut nitrogen losses in growing pigs are not caused by increased protein synthesis rates in the small intestine. Journal of Nutrition 130, 566–572.CrossRefGoogle Scholar
Preston, T & McMillan, DC (1988) Rapid sample throughput for biomedical stable isotope tracer studies. Biomedical and Environmental Mass Spectrometry 16, 229–235.CrossRefGoogle ScholarPubMed
Rahman, MH, Hossain, MI & Moslehuddin, (1997) Nutritional evaluation of sweet lupin (Lupinus angustifolius): net protein utilization, nitrogen balance and fractionation studies. British Journal of Nutrition 77, 443–457.CrossRefGoogle Scholar
Rubio, LA, Grant, G, Cavallé, C, Martinez-Aragon, A & Pusztai, A (1994) High in-vivo (rat) digestibility of faba bean (Vicia faba), lupin (Lupinus angustifolius) and soya bean (Glycine max) soluble globulins. Journal of the Science of Food and Agriculture 66, 289–292.CrossRefGoogle Scholar
Rubio, LA, Grant, G, Scislowski, PW, Brown, D, Bardocz, S & Pusztai, A (1995) The utilization of lupin (Lupinus angustifolius) and faba bean globulins by rats is poorer than of soybean globulins or lactalbumin but the nutritional value of lupin seed meal is lower only than that of lactalbumin. Journal of Nutrition 125, 2145–2155.CrossRefGoogle ScholarPubMed
Sauer, WC, Mosenthin, R, Ahrens, F & den Hartog, LA (1991) The effect of source of fiber on ileal and fecal amino acid digestibility and bacterial nitrogen excretion in growing pigs. Journal of Animal Science 69, 4070–4077.CrossRefGoogle ScholarPubMed
Schulze, H, van Leeuwen, P, Verstegen, MW, Huisman, J, Souffrant, WB & Ahrens, F (1994) Effect of level of dietary neutral detergent fiber on ileal apparent digestibility and ileal nitrogen losses in pigs. Journal of Animal Science 72, 2362–2368.CrossRefGoogle ScholarPubMed
Sgarbieri, VC & Galeazzi, MAM (1978) Some physicochemical and nutritional properties of a sweet lupin (Lupinus albus var. multolupa) protein. Journal of Agricultural and Food Chemistry 26, 1438–1442.CrossRefGoogle ScholarPubMed
Tetens, I, Livesey, G & Eggum, BO (1996) Effects of the type and level of dietary fibre supplements on nitrogen retention and excretion patterns. British Journal of Nutrition 75, 461–469.CrossRefGoogle ScholarPubMed
Tomé, D & Bos, C (2000) Dietary protein and nitrogen utilization. Journal of Nutrition 130, 1868S–1873S.Google ScholarPubMed
van Barneveld, RJ (1999) Understanding the nutritional chemistry of lupin (Lupinus spp) seed to improve livestock production efficiency. Nutrition Research Reviews 12, 203–230.CrossRefGoogle ScholarPubMed
Younes, H, Garleb, K, Behr, S, Rémésy, C & Demigné, C (1995) Fermentable fibers or oligosaccharides reduce urinary nitrogen excretion by increasing urea disposal in the rat cecum. Journal of Nutrition 125, 1010–1016.Google ScholarPubMed
Young, VR (1991) Nutrient interactions with reference to amino acid and protein metabolism in non-ruminants; particular emphasis on protein-energy relations in man. Zeitschrift fur Ernahrungswissenschaft 30, 239–267.CrossRefGoogle ScholarPubMed
Young, VR & Borgonha, S (2000) Nitrogen and amino acid requirements: the Massachusetts Institute of Technology amino acid requirement pattern. Journal of Nutrition 130, 1841S–1849S.CrossRefGoogle ScholarPubMed
Young, VR, Scrimshaw, NS & Bier, DM (1981) Whole body protein and amino acid metabolism: relation to protein quality evaluation in human nutrition. Journal of Agricultural and Food Chemistry 29, 440–447.CrossRefGoogle ScholarPubMed