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Beef conjugated linoleic acid isomers reduce human cancer cell growth even when associated with other beef fatty acids

Published online by Cambridge University Press:  08 March 2007

Anne De La Torre ,
Eric Debiton ,
Pierre Juanéda ,
Denys Durand ,
Jean-Michel Chardigny ,
Chantal Barthomeuf ,
Dominique Bauchart  and
Dominique Gruffat
Anne De La Torre
Affiliation:
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genés-Champanelle, France
Eric Debiton
Affiliation:
Laboratoire de Pharmacognosie/Biotechnologies, UMR 484 INSERM, 63005 Clermont-Ferrand, France
Pierre Juanéda
Affiliation:
INRA, Unité de Nutrition Lipidique, 21065 Dijon, France
Denys Durand
Affiliation:
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genés-Champanelle, France
Jean-Michel Chardigny
Affiliation:
INRA, Unité de Nutrition Lipidique, 21065 Dijon, France
Chantal Barthomeuf
Affiliation:
Laboratoire de Pharmacognosie/Biotechnologies, UMR 484 INSERM, 63005 Clermont-Ferrand, France
Dominique Bauchart
Affiliation:
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genés-Champanelle, France
Dominique Gruffat*
Affiliation:
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genés-Champanelle, France
*
*corresponding author: fax +33 04 73 62 46 39, email [email protected]
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Abstract

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Although many data are available concerning anticarcinogenic effects of industrial conjugated linoleic acid (CLA), few studies have reported the antitumour properties of CLA mixtures originating from ruminant products. The aim of the present study was to investigate the in vitro antiproliferative effects of beef CLA mixtures on breast, lung, colon, melanoma and ovarian human cancer cell lines. For this purpose, four fatty acid (FA) extracts prepared from beef lipid and varying in their CLA composition, their corresponding purified CLA-enriched fractions, and mixtures of pure synthetic CLA, the composition of which reproduced that of the four selected beef samples, were tested on cancer cell lines. Cancer cells were exposed for 48h to medium containing 100μm-FA and their proliferation was determined by quantifying cellular DNA content (Hoechst 33342 dye). Compared with cells incubated without FA, the number of cancer cells was reduced from 25 to 67% (P<0·0001) following FA treatment. Antiproliferative effects of CLA mixtures varied in magnitude according to the source of FA, the CLA composition and the cell lines. CLA mixtures naturally present in beef inhibited the proliferation of human cancer cell lines, a high content in cis-trans isomers allowing the most important antiproliferative effect. Beef total FA exhibited a greater growth-inhibitory activity than their corresponding CLA-enriched fractions. These results suggested that either beef FA other than beef CLA could possess antiproliferative properties and/or the existence of complementary effects of non-conjugated FA and CLA, which could favour the antiproliferative properties of beef total FA.

Keywords

BeefFatty acidsConjugated linoleic acidHuman cancer cellsGrowth inhibition
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 2 , February 2006 , pp. 346 - 352
Copyright
Copyright © The Nutrition Society 2006

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