Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-22T20:58:24.194Z Has data issue: false hasContentIssue false
  • English
  • Français

Antioxidant vitamin supplements do not reduce reactive oxygen species activity in Helicobacter pylori gastritis in the short term

Published online by Cambridge University Press:  09 March 2007

Simon M. Everett ,
Ian M. Drake ,
Kay L. M. White ,
Nic P. Mapstone ,
Douglas M. Chalmers ,
Chris J. Schorah  and
Anthony T. R. Axon
Simon M. Everett*
Affiliation:
The Centre for Digestive Diseases, The General Infirmary at Leeds, Great George Street, Leeds LS1 3EX, UK
Ian M. Drake
Affiliation:
Chorley and South Ribble District General Hospital, Preston Road, Chorley, Lancashire PR7 1PP, UK
Kay L. M. White
Affiliation:
The Centre for Digestive Diseases, The General Infirmary at Leeds, Great George Street, Leeds LS1 3EX, UK
Nic P. Mapstone
Affiliation:
The Centre for Digestive Diseases, The General Infirmary at Leeds, Great George Street, Leeds LS1 3EX, UK
Douglas M. Chalmers
Affiliation:
The Centre for Digestive Diseases, The General Infirmary at Leeds, Great George Street, Leeds LS1 3EX, UK
Chris J. Schorah
Affiliation:
The Centre for Digestive Diseases, The General Infirmary at Leeds, Great George Street, Leeds LS1 3EX, UK
Anthony T. R. Axon
Affiliation:
The Centre for Digestive Diseases, The General Infirmary at Leeds, Great George Street, Leeds LS1 3EX, UK
*
Corresponding author: Dr Simon M. Everest, fax +44 113 242 9722, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Reactive oxygen species have been implicated in Helicobacter pylori-mediated gastric carcinogenesis, whereas diets high in antioxidant vitamins C and E are protective. We have examined the effect of vitamin C and E supplements in combination with H. pylori eradication on reactive oxygen species activity in H. pylori gastritis. H. pylori-positive patients were randomized into four groups: triple therapy alone (Bismuth chelate, tetracycline, and metronidazole for 2 weeks), vitamins alone (200 mg vitamin C and 50 mg vitamin E, both twice per day for 4 weeks), both treatments or neither. Plasma and mucosal ascorbic acid, malondialdehyde and reactive oxygen species were determined before and after treatment. Compared with normal controls (n 61), H. pylori-positive patients (n 117) had higher mucosal reactive oxygen species and malondialdehyde levels and lower plasma ascorbic acid. Plasma ascorbic acid doubled in both groups of patients receiving vitamins and mucosal levels also increased. Malondialdehyde and reactive oxygen species fell in patients in whom H. pylori was eradicated but vitamin supplements were not effective either alone or in combination with H. pylori eradication. Supplements of vitamins C and E do not significantly reduce mucosal reactive oxygen species damage in H. pylori gastritis.

Keywords

Ascorbic acidVitamin CVitamin EReactive oxygen speciesMalondialdehydeGastric carcinomaHelicobacter pylori
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 1 , January 2002 , pp. 3 - 11
Copyright
Copyright © The Nutrition Society 2002

References

Banerjee, S, Hawksby, C, Miller, S, Dahill, S, Beattie, A & McColl, K (1994) Effect of Helicobacter pylori and its eradication on gastric juice ascorbic acid. Gut 35, 317–322.CrossRefGoogle ScholarPubMed
Block, G (1991) Vitamin C and cancer prevention: the epidemiologic evidence. American Journal of Clinical Nutrition 53, 270S–282S.CrossRefGoogle ScholarPubMed
Brennan, LA, Morris, GM, Wasson, GR, Hannigan, BM & Barnett, YA (2000) The effect of vitamin C or vitamin E supplementation on basal and H2O2 induced DNA damage in human lymphocytes. British Journal of Nutrition 84, 195–202.CrossRefGoogle ScholarPubMed
Cadenas, S, Lertsiri, S, Otsuka, M, Barja, G & Miyazawa, T (1996) Phospholipid hydroperoxides and lipid peroxidation in liver and plasma ODS rats supplemented with alpha-tocopherol and ascorbic acid. Free Radical Research 24, 485–493.CrossRefGoogle ScholarPubMed
Correa, P (1995) The role of antioxidants in gastric carcinogenesis. Critical Reviews in Food Science and Nutrition 35, 59–64.CrossRefGoogle ScholarPubMed
Davies, GR, Simmonds, NJ, Stevens, TRJ, Sheaff, MT, Banatvala, N, Laurenson, IF, Blake, DR & Rampton, DS (1994) Helicobacter pylori stimulates antral mucosal reactive oxygen metabolite production in vivo. Gut 35, 179–185.CrossRefGoogle ScholarPubMed
Dixon, MF, Genta, RM & Yardley, JH (1996) Correa P and the participants of the International Workshop on the Histopathology of Gastritis Classification and grading of gastritis. The updated Sydney system. American Journal of Surgical Pathology 20, 1161–1181.CrossRefGoogle Scholar
Drake, I, Davies, M, Mapstone, N, Dixon, M, Schorah, C, White, K, Chalmers, D & Axon, A (1996) Ascorbic acid may protect against human gastric cancer by scavenging mucosal oxygen radicals. Carcinogenesis 17, 559–562.CrossRefGoogle ScholarPubMed
Drake, I, Mapstone, N, Schorah, C, White, K, Chalmers, D, Dixon, M & Axon, A (1998) Reactive oxygen species activity and lipid peroxidation in Helicobacter pyloriH. pylori eradication. Gut 42, 768–771.CrossRefGoogle Scholar
Dyke, G, Craven, J, Hall, R & Garner, R (1994) Effect of vitamin C supplementation on gastric mucosal DNA damage. Carcinogenesis 15, 291–295.CrossRefGoogle ScholarPubMed
Emma, AM & Fitzgerald, GA (2000) Indices of lipid peroxidation in vivo: strengths and limitations. Free Radical Biology and Medicine 28, 1745–1750.Google Scholar
Everett, SM, Singh, R, Leuratti, C, White, KLM, Neville, P, Greenwood, D, Marnett, LJ, Schorah, CJ, Forman, D, Shuker, D & Axon, ATR (2001) Levels of malondialdehyde-deoxyguanosine in the gastric mucosa - relationship with lipid peroxidation, ascorbic acid and Helicobacter pylori. Cancer Epidemiology and Biomarkers 10, 369–376.Google ScholarPubMed
Farinati, F, Della Libera, G, Cardin, R, Molari, A, Plebani, M, Rugge, M, Di Mario, F & Naccarato, R (1996) Gastric antioxidant, nitrites, and mucosal lipoperoxidation in chronic gastritis and Helicobacter pylori infection. Journal of Clinical Gastroenterology 22, 275–281.CrossRefGoogle ScholarPubMed
Frei, B (1991) Ascorbic acid protects lipids in human plasma and low density lipoprotein against oxidative damage. American Journal of Clinical Nutrition 54, 1113S–1118S.CrossRefGoogle ScholarPubMed
Goodman, K, Correa, P, Tengana Aux, H, DeLany, J & Collazos, T (1997) Nutritional factors and Helicobacter pylori infection in Colombian children. Journal of Pediatric Gastroenterology and Nutrition 25, 507–515.Google ScholarPubMed
Guyton, KZ & Kensler, TW (1993) Oxidative mechanisms in carcinogenesis. British Medical Bulletin 49, 523–544.CrossRefGoogle ScholarPubMed
Halliwell, B & Gutteridge, J (1998) Free Radicals in Biology and Medicine, Oxford: Oxford University Press.Google Scholar
Herbert, V (1994) The antioxidant supplement myth. American Journal of Clinical Nutrition 60, 157–158.CrossRefGoogle ScholarPubMed
International Agency for Research on Cancer (1994) IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Shistosomes, Liver Flukes and Helicobacter pylori. Monograph 61. Lyon: IARC Scientific Publications.Google Scholar
Jackson, JH, Gajewski, E, Schraufstatter, IU, Hyslop, PA, Fuciarelli, AF, Cochrane, CG & Dizdaroglu, M (1989) Damage to the bases in DNA induced by stimulated human neutrophils. Journal of Clinical Investigation 84, 1644–1649.CrossRefGoogle Scholar
Jarosz, M, Dzieniszewski, J, Dabrowska-Ufniarz, E, Wartanowicz, M, Ziemlariski, S & Reed, PI (1998) Effects of high vitamin C doses on Helicobacter pylori infection and total vitamin C concentration in gastric juice. European Journal of Cancer Prevention 7, 449–454.CrossRefGoogle Scholar
Knekt, P, Aromaa, A, Maatela, J, Aaran, R, Nikkari, T, Hakama, M, Hakulinen, T, Peto, R & Teppo, L (1991) Vitamin E and cancer prevention. American Journal of Clinical Nutrition 53, 283S–286S.CrossRefGoogle ScholarPubMed
La Vecchia, C, Ferraroni, M, D'Avanzo, B, Decarli, A & Franceschi, S (1994) Selected micronutrient intake and the risk of gastric cancer. Cancer, Epidemiology, Biomarkers and Prevention 3, 393–398.Google ScholarPubMed
Lee, SH, Oe, T & Blair, IA (2001) Vitamin C-induced decomposition of lipid hydroperoxides to endogenous genotoxins. Science 292, 2083–2086.CrossRefGoogle ScholarPubMed
Levine, M, Conry-Cantilena, C, Wang, Y, Welch, R, Washko, P, Dhariwal, K, Park, J, Laxarev, A, Graumilch, J, King, J & Cantilena, L (1996) Vitamin C pharmacokinetics in healthy volunteers: Evidence for a recommended dietary allowance. Proceedings of the National Academy of Science, USA 93, 3704–3709.CrossRefGoogle ScholarPubMed
Marnett, LJ, (1994) DNA adducts of α, β-unsaturated aldehydes and dicarbonyl compounds. In DNA Adducts: Identification and Biological Significance Vol 125, 151–163 [Hemminki, K, Dipple, A, Shuker, DEG, Kadlubar, FF, Segerback, D and Bartsch, H editors]. Lyon: IARC Scientific Publications.Google Scholar
Mirvish, S, Wallcave, L, Eagen, M & Shubik, P (1972) Ascorbate–Nitrite reaction: Possible means of blocking the formation of carcinogenic N-nitroso compounds. Science 177, 65–67.CrossRefGoogle ScholarPubMed
Omenn, G, Goodman, G, Thornquist, M, Balmes, J, Cullen, MR, Glass, A, Keogh, JP, Meyskens, FL Jr, Valanis, B, Williams, JH Jr, Barnhart, S, Cherniack, MG, Brodkin, CA & Hammar, S (1996) Risk factors for lung cancer and for intervention effects in CARET, the Beta-Carotene and Retinal Efficacy Trial. Journal of the National Cancer Institute 88, 1550–1559.CrossRefGoogle Scholar
Parkin, DM, Pisani, P & Ferlay, J (1993) Estimates of worldwide incidence of eighteen major cancers in 1985. International Journal of Cancer 54, 594–606.CrossRefGoogle ScholarPubMed
Phull, P, Price, A, Thorniley, M, Green, C & Jacyna, M (1998) Plasma free radical activity and antioxidant vitamin levels in dyspeptic patients: correlation with smoking and Helicobacter pylori infection. European Journal of Gastroenterology and Hepatology 10, 573–577.CrossRefGoogle ScholarPubMed
Simmonds, N, Allen, R, Stevens, T, van Someren, N, Blake, D & Rampton, D (1992) Chemiluminescence assay of mucosal reactive oxygen metabolites in inflammatory bowel disease. Gastroenterology 103, 186–196.CrossRefGoogle ScholarPubMed
Sobala, G, Schorah, C, Sanderson, M, Dixon, M, Tompkins, D, Godwin, P & Axon, A (1989) Ascorbic acid in the human stomach. Gastroenterology 97, 357–363.CrossRefGoogle ScholarPubMed
Sobala, GM, Pignatelli, B, Schorah, CJ, Bartsch, H, Sanderson, M, Dixon, MF, Shire, S, King, RFG & Axon, ATR (1991) Levels of nitrite, nitrate, N-nitroso compounds, ascorbic acid and total bile acids in gastric juice of patients with and without precancerous conditions of the stomach. Carcinogenesis 12, 193–198.CrossRefGoogle ScholarPubMed
Srigiridhar, K & Nair, KM (2000) Supplementation with α-tocopherol or a combination of α-tocopherol and ascorbic acid protects the gastrointestinal tract of iron-deficient rats against iron-induced oxidative damage during iron repletion. British Journal of Nutrition 84, 165–173.CrossRefGoogle ScholarPubMed
The Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study Group (1994) The effects of vitamin E and beta carotene on the incidence of lung cancer and other cancers in male smokers. New England Journal of Medicine 12, 289–303.Google Scholar
Waring, A, Drake, I, Schorah, C, White, K, Lynch, D, Axon, A & Dixon, M (1996) Ascorbic acid and total vitamin C concentrations in plasma, gastric juice, and gastrointestinal mucosa: effects of gastritis and oral supplementation. Gut 38, 171–176.CrossRefGoogle ScholarPubMed
Yagi, K (1976) A simple fluorimetric assay for lipoperoxide in blood plasma. Biochemical Medicine 15, 212–216.CrossRefGoogle Scholar