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Antioxidant vitamin status in high exposure to oxidative stress in competitive athletes

Published online by Cambridge University Press:  09 March 2007

Anne-Sophie Rousseau ,
Isabelle Hininger ,
Stéphane Palazzetti ,
Henri Faure ,
Anne-Marie Roussel  and
Irène Margaritis
Anne-Sophie Rousseau
Affiliation:
Laboratoire Nutritiion, Vieillissement et Maladies Cardio-vasculaires, Université Joseph Fourier, Domaine de la Merci, La Tronche, France
Isabelle Hininger
Affiliation:
Laboratoire Nutritiion, Vieillissement et Maladies Cardio-vasculaires, Université Joseph Fourier, Domaine de la Merci, La Tronche, France
Stéphane Palazzetti
Affiliation:
Laboratoire Physiologie des Adaptations, Performance Motrice et Santé, Université de Nice-Sophia-Antipolis, France
Henri Faure
Affiliation:
Laboratoire Nutritiion, Vieillissement et Maladies Cardio-vasculaires, Université Joseph Fourier, Domaine de la Merci, La Tronche, France
Anne-Marie Roussel
Affiliation:
Laboratoire Nutritiion, Vieillissement et Maladies Cardio-vasculaires, Université Joseph Fourier, Domaine de la Merci, La Tronche, France
Irène Margaritis*
Affiliation:
Laboratoire Physiologie des Adaptations, Performance Motrice et Santé, Université de Nice-Sophia-Antipolis, France
*
*Corresponding author: fax +33 4 92 29 65 02, Email [email protected]
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Abstract

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We conducted a cross-sectional study in 118 well-trained athletes to investigate 'high exposure' to sub-deficient antioxidant status, and consequently to oxidative damage, in relation to estimated daily energy expenditure (EE) and dietary antioxidant intake. Subjects completed 7 d food and activity records. Blood samples were obtained on day 8. Of the athletes 81, 60 and 43 % had intakes of vitamins E, C and β-carotene below two-thirds of the French RDA respectively, which is adjusted for EE (FRDAa). The deficit in vitamin E intake was positively correlated with EE (r 0·51, P>0·0001). All the athletes had normal plasma vitamins E and C and 14 % had marginal plasma β-carotene. Plasma thiobarbituric acid-reactive substances (TBARS) did not increase with increased EE. As evidenced by ANOVA, EE-induced vitamin C intakes increased and consequently led to increased plasma ascorbic acid concentrations. In male athletes, plasma total carotenoids were negatively correlated with plasma TBARS concentrations (r –0·31, P>0·006). The relationship between vitamin C intakes and plasma concentrations was logarithmic (r 0·59, P> 0·0001). To summarize, it is not clear whether vitamin E requirements are overestimated with reference to EE in the FRDAa. Daily requirements for vitamin C do not exceed 200 mg. Our present results could be interpreted as meaning that carotenoids play a protective role as exogenous antioxidants. Carotenoid intakes in athletes must be considered carefully.

Keywords

Dietary assessmentAntioxidant vitaminsCarotenoidsOxidative stressPhysical activity
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 3 , September 2004 , pp. 461 - 468
Copyright
Copyright © The Nutrition Society 2004

References

Aguilo, A, Tauler, P, Pilar Guix, M, Villa, G, Cordova, A, Tur, JA & Pons, AEffect of exercise intensity and training on antioxidants and cholesterol profile in cyclists. J Nutr Biochem (2003) 14, 319–325.CrossRefGoogle ScholarPubMed
Aikawa, KM, Quintanilha, AT, de Lumen, BO, Brooks, GA & Packer, LExercise endurance-training alters vitamin E tissue levels and red-blood-cell hemolysis in rodents. Biosci Rep (1984) 4, 253–257.CrossRefGoogle ScholarPubMed
Ainsworth, BE, Haskell, WL & Whitt, MCCompendium of physical activities: an update of activity codes and MET intensities. Med Sci Sports Exerc (2000) 32, S498–S504.CrossRefGoogle ScholarPubMed
Arnaud, J, Fortis, I, Blachier, S, Kia, D & Favier, ASimultaneous determination of retinol, alpha-tocopherol and beta-carotene in serum by isocratic high-performance liquid chromatography. J Chromatogr B (1991) 572, 103–116.CrossRefGoogle ScholarPubMed
Balakrishnan, DS & Anuradha, CVExercise, depletion of antioxidants and antioxidant manipulation. Cell Biochem Funct (1998) 16, 269–275.3.0.CO;2-B>CrossRefGoogle ScholarPubMed
Bergholm, R, Makimattila, S, Valkonen, M, Liu, ML, Lahdenpera, S, Taskinen, MR, Sovijarvi, A, Malmberg, P & Yki-Jarvinen, HIntense physical training decreases circulating antioxidants and endothelium-dependent vasodilatation in vivo. Atherosclerosis (1999) 145, 341–349.CrossRefGoogle ScholarPubMed
Blundell, JE & King, NAPhysical activity and regulation of food intake: current evidence. Med Sci Sports Exerc (1999) 31, S573–S583.CrossRefGoogle ScholarPubMed
Bowles, DK, Torgan, CE, Ebner, S, Kehrer, JP, Ivy, JL & Starnes, JWEffects of acute, submaximal exercise on skeletal muscle vitamin E. Free Radic Res Commun (1991) 14, 139–143.CrossRefGoogle ScholarPubMed
Brites, FD, Evelson, PA, Christiansen, MG, Nicol, MF, Basilico, MJ, Wikinski, RW & Llesuy, SFSoccer players under regular training show oxidative stress but an improved plasma antioxidant status. Clin Sci (1999) 96, 381–385.CrossRefGoogle ScholarPubMed
Cazzola, R, Russo-Volpe, S, Cervato, G & Cestaro, BBiochemical assessments of oxidative stress, erythrocyte membrane fluidity and antioxidant status in professional soccer players and sedentary controls. Eur J Clin Invest (2003) 33, 924–930.CrossRefGoogle ScholarPubMed
Child, RB, Wilkinson, DM & Fallowfield, JLResting serum antioxidant status is positively correlated with peak oxygen uptake in endurance trained runners. J Sports Med Phys Fitness (1999) 39, 282–284.Google ScholarPubMed
Evelson, P, Gambino, G, Travacio, M, Jaita, G, Verona, J, Maroncelli, C, Wikinski, R, Llesuy, S & Brites, FHigher antioxidant defences in plasma and low density lipoproteins from rugby players. Eur J Clin Invest (2002) 32, 818–825.CrossRefGoogle ScholarPubMed
Gohil, K, Rothfuss, L, Lang, J & Packer, LEffect of exercise training on tissue vitamin E and ubiquinone content. J Appl Physiol (1987) 63, 1638–1641.CrossRefGoogle ScholarPubMed
Guilland, JC, Margaritis, I, Melin, B, Pérès, G, Richalet, JP & Sabatier, pp Sportif et sujets á activité physique intense (Sportsmen and subjects with high physical activity). In Apports Nutritionnels Conseillé pour la Population Française (Recommended Dietary Allowances for the French Population), 3rd ed. pp. 337–394. Paris: Tec & Doc. (2001)Google Scholar
Hercberg, S, Preziosi, P, Galan, P, Deheeger, M, Papoz, L & Dupin, HConsommation alimentaire d'un échantillon représentatif de la population du Val de Marne. III. Apports en minéraux et vitamins (Dietary intake of a representative sample of the population of Val-de-Marne; III. Mineral and vitamin intake). Rev Epidemiol Sante Publique (1991) 39, 245–261Google Scholar
Itoh, H, Ohkuwa, Y, Shimoda, T, Wakayama, A, Tamura, S, Yamamoto, T, Sato, Y & Miyamura, MVitamin E supplementation attenuates leakage of enzymes following 6 successive days of running training. Int J Sports Med (1999) 21, 369–374.CrossRefGoogle Scholar
Ji, LLOxidative stress during exercise: implication of antioxidant nutrients. Free Radic Biol Med (1995) 18, 1079–1086.CrossRefGoogle ScholarPubMed
Ji, LLAntioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med (1999) 222, 283–292.CrossRefGoogle ScholarPubMed
Krinsky, NI & Yeum, KCarotenoid-radical interactions. Biochem Biophys Res Commun (2003) 305, 754–760.CrossRefGoogle ScholarPubMed
Le Moullec, N, Deheeger, M, Preziosi, P, Montero, P, Valeix, P, Rolland-Cachera, MF, Potier de Courcy, G, Christides, JP, Galan, P & Hercberg, SValidation du manuel photos utilisé pour l'enquête alimentaire de l'étude SU.VI.MAX. (Validation of the photo manual used for the collection of dietary data in the SU.VI.MAX Study). Cah Nutr Diet (1996) 31, 158–164 (in French).Google Scholar
Levine, M, Conry-Cantilena, C & Wang, YVitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance. Proc Natl Acad Sci USA (1996) 93, 3704–3709.CrossRefGoogle ScholarPubMed
Levine, M, Wang, Y, Padayatty, SJ & Morrow, JA new recommended dietary allowance of vitamin C for healthy young women. Proc Natl Acad Sci USA (2001) 98, 9842–9846.CrossRefGoogle ScholarPubMed
Linseisen, J, Metges, CC & Wolfram, GDietary habits and serum lipids of a group of German amateur body-builders. Z Ernahrungswiss (1993) 32, 289–300.CrossRefGoogle ScholarPubMed
Marzatico, F, Pansarasa, O, Bertorelli, L, Somenzini, L & Della Valle, GBlood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes. J Sports Med Phys Fitness (1997) 37, 235–239.Google ScholarPubMed
Olmedilla, B, Granado, F & Southon, SSerum concentration of carotenoids and vitamins A, E and C in control subjects from five European countries. Br J Nutr (2001) 85, 227–238.CrossRefGoogle Scholar
O'Neill, ME, Carroll, Y & Corridan, BA European carotenoid database to assess carotenoid intakes and its use in a five-country comparative study. Br J Nutr (2001) 85, 499–507.CrossRefGoogle Scholar
Pincemail, J, Lecomte, J, Castiau, JP, Collard, E, Vasankari, T, Cheramy-Bien, JP, Limet, R & Defraigne, JOEvaluation of autoantibodies against oxidized LDL and antioxidant status in top soccer and basketball players after 4 months of competition. Free Radic Biol Med (2000) 28, 559–565.CrossRefGoogle ScholarPubMed
Polidori, MC, Mecocci, P, Cherubini, A & Senin, UPhysical activity and oxidative stress during aging. Int J Sports Med (2000) 21, 154–157.CrossRefGoogle ScholarPubMed
Powers, SK, Criswell, D, Lawler, J, Ji, LL, Martin, D, Herb, RA & Dudley, GInfluence of exercise and fiber type on antioxidant enzyme activity in rat skeletal muscle. Am J Physiol (1994) 266, R375–R380.Google ScholarPubMed
Prior, RLFruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr (2003) 78, 570S–578S.CrossRefGoogle ScholarPubMed
Richard, MJ, Guiraud, P, Meo, J & Favier, AHigh-performance liquid chromatographic separation of malondialdehyde-thiobarbituric acid adduct in biological materials (plasma and human cells) using a commercially available reagent. J Chromatogr (1992 a) 577, 9–18.CrossRefGoogle ScholarPubMed
Richard, MJ, Portal, B, Meo, J, Coudray, C, Hadjian, A & Favier, AMalondialdehyde kit evaluated for determining plasma and lipoprotein fractions that react with thiobarbituric acid. Clin Chem (1992 b) 38, 704–709.CrossRefGoogle ScholarPubMed
Rokitzki, L, Logemann, E, Huber, G, Keck, E & Keul, JAlpha-tocopherol supplementation in racing cyclists during extreme endurance training. Int J Sport Nutr (1994) 4, 253–264.CrossRefGoogle ScholarPubMed
Schröder, H, Navarro, E, Tramullas, A, Mora, J & Galiano, DNutrition antioxidant status and oxidative stress in professional basketball players: effects of a three compound antioxidative supplement. Int J Sports Med (2000) 21, 146–150.CrossRefGoogle ScholarPubMed
Singh, A, Evans, P, Gallagher, KL & Deuster, PADietary intakes and biochemical profiles of nutritional status of ultramarathoners. Med Sci Sports Exerc (1993) 25, 328–334.CrossRefGoogle ScholarPubMed
Speek, AJ, Schrijver, J, Schreus, WHPFluorometric determination of total vitamin C in whole blood by high performance liquid chromatography with pre-column derivatization. J Chromatogr (1984) 305, 53–60.CrossRefGoogle ScholarPubMed
Starnes, JW, Cantu, G, Farrar, RP & Kehrer, JPSkeletal muscle lipid peroxidation in exercised and food-restricted rats during aging. J Appl Physiol (1989) 67, 69–75.CrossRefGoogle ScholarPubMed
Swift, JN, Jr Kehrer, JPSeiler, KS & Starnes, JWVitamin E concentration in rat skeletal muscle and liver after exercise. Int J Sport Nutr (1998) 8, 105–112.CrossRefGoogle ScholarPubMed
Urso, ML & Clarkson, PMOxidative stress, exercise, and antioxidant supplementation. Toxicology (2003) 18, 41–54.CrossRefGoogle Scholar