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Antioxidant supplementation and exercise-induced oxidative stress in the 60-year-old as measured by antipyrine hydroxylates

Published online by Cambridge University Press:  09 March 2007

Erwin P. Meijor ,
Annelies H. C. Goris ,
Joan Senden ,
Joost L. J. van Dongen ,
Aalt Bast  and
Klass R. Westerterp
Erwin P. Meijor*
Affiliation:
Department of Human Biology, Maastricht University,, Maastricht,, The Netherlands
Annelies H. C. Goris
Affiliation:
Department of Human Biology, Maastricht University,, Maastricht,, The Netherlands
Joan Senden
Affiliation:
Department of Human Biology, Maastricht University,, Maastricht,, The Netherlands
Joost L. J. van Dongen
Affiliation:
Laboratory for Macromolecular and Organic Chemistry, Eindhoven University of Technology, Eindhoven, The Netherlands
Aalt Bast
Affiliation:
Department of Pharmacology and Toxicology, Maastricht University, Maastricht, The Netherland
Klass R. Westerterp
Affiliation:
Department of Human Biology, Maastricht University,, Maastricht,, The Netherlands
*
*Corresponding author: Dr Erwin P. Meijer, fax +31 43 3670976, email [email protected]
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Abstract

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The effects of 12 weeks of antioxidant supplementation on exercise-induced oxidative stress were investigated in older adults (60 (SE 1) YEARS; BMI 26 (se 1) kg/m2). Subjects were randomly divided in two groups: supplementation (n 11) with 100 mg dl-α-tocopheryl acetate, 200 mg ascorbic acid, and 2 mg β-carotene, and placebo (n 9). Before and after the 12 week supplementation period, subjects cycled for 45 min at submaximal intensity (50 % maximal workload capacity). Antipyrine was used as marker for oxidative stress. Antipyrine reacts quickly with hydroxyl radicals to form para- and ortho-hydroxyantipyrine. The latter metabolite is not formed in man through the mono-oxygenase pathway of cytochrome P450. Daily supplementation significantly increased plasma concentrations of α-tocopherol and β-carotene in the supplemented group (Δ 14·4 (se 3·2) and 0·4 (se 0·1) μmol/l; P<0·001 and P<0·01). No significant differences, within and between groups, were observed in the exercise-induced increase in the ratios para- and ortho-hydroxyantipyrine to antipyrine. In addition, supplementation did not affect the exercise-induced increase in thiobarbituric acid reactive substances in plasma. In conclusion, in 60-year-old subjects antioxidant supplementation had no effect on the exercise-induced increase in oxidative stress as measured by free radical products of antipyrine.

Keywords

AgeingAntipyrineFree radicalsAntioxidant vitamins
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 5 , November 2001 , pp. 569 - 575
Copyright
Copyright © The Nutrition Society 2001

References

Referenses

Ashton, T, Young, IS, Peters, JR, Jones, E, Jackson, SK, Davies, B & Rowlands, CC (1999) Electron spin resonance spectroscopy, exercise and oxidative stress: an ascorbic acid intervention study. Journal of Applied Physiology 87, 2032–2036.CrossRefGoogle ScholarPubMed
Bast, A, Haenen, GRMM, Van Den Berg, R & Van Den Berg, H (1998) Antioxidant effects of carotenoids. International Journal for Vitamin and Nutrition Research 68, 399–403.Google ScholarPubMed
Beckman, KB & Ames, BN (1998) The free radical theory of aging matures. Physiological Reviews 78, 547–581.CrossRefGoogle ScholarPubMed
Brigelius-Flohé, R & Traber, MG (1999) Vitamin E: function and metabolism. FASEB Journal 13, 1145–1155.CrossRefGoogle Scholar
Clarkson, PM & Thompson, HS (2000) Antioxidants: what role do they play in physical activity and health?. American Journal of Clinical Nutrition 72, 637S–646S.CrossRefGoogle ScholarPubMed
Coolen, SAJ (2000) Antipyrine hydroxylates as indicators for oxidative damage (PhD thesis) Eindhoven University of Technology.Google Scholar
Coolen, SAJ, Everaerts, FM & Huf, FA (1997) Characterization of 60Co γ-radiation induced radical products of antipyrine by means of high-performance liquid chromatography, mass spectrometry, capillary zone electrophoresis, micellar electrokinetic capillary chromatography and nuclear magnetic resonance spectrometry. Journal of Chromatography A 788, 95–103.Google Scholar
Coolen, SAJ, Van Lieshout, M, Reijenga, JC & Huf, FA (1999) Determination of phenolic derivatives of antipyrine in plasma with HPLC-Tandem MS using ESI and Turbo ionspray as interfaces. Journal of Microcolumn Separations 11, 701–707.3.0.CO;2-3>CrossRefGoogle Scholar
Davies, KJ, Quintanilha, AT, Brooks, GA & Packer, L (1982) Free radicals and tissue damage produced by exercise. Biochemical and Biophysical Research Communications 107, 1198–1205.Google Scholar
Forni, LG, Mora-Arellano, VO, Packer, JE & Willson, RL (1988) Aminopyrine and antipyrine free radical-cations: pulse radiolysis studies of one-electron transfer reactions Journal of the Chemical Society-Perkin Transactions II 1579–1584.CrossRefGoogle Scholar
Fotouhi, N, Meydani, M, Santos, MS, Meydani, SN, Hennekens, CH & Gaziano, JM (1996) Carotenoid and tocopherol concentrations in plasma, peripheral blood mononuclear cells, and red blood cells after long-term β-carotene supplementation in men. American Journal of Clinical Nutrition 63, 553–558.Google Scholar
Goris, AH & Westerterp, KR (1999) Underreporting of habitual food intake is explained by undereating in highly motivated lean women. Journal of Nutrition 129, 878–882.CrossRefGoogle ScholarPubMed
Hartleb, M (1991) Drugs and the liver. Part II. The role of the antipyrine test in drug metabolism studies. Biopharmaceutics and Drug Disposition 12, 559–570.CrossRefGoogle ScholarPubMed
Hartmann, A, Niess, AM, Grunert Fuchs, M, Poch, B & Speit, G (1995) Vitamin E prevents exercise-induced DNA damage. Mutation Research 346, 195–202.CrossRefGoogle ScholarPubMed
Hess, D, Keller, HE, Oberlin, B, Bonfanti, R & Schuep, W (1991) Simultaneous determination of retinol, tocopherols, carotenes and lycopene in plasma by means of high-performance liquid chromatography on reversed phase. International Journal for Vitamin and Nutrition Research 61, 232–238.Google Scholar
Hughes, DA (1999) Effects of dietary antioxidants on the immune function of middle-aged adults. Proceedings of the Nutrition Society 58, 79–84.CrossRefGoogle ScholarPubMed
Jacques, PF, Halpner, AD & Blumberg, JB (1995) Influence of combined antioxidant nutrient intakes on their plasma concentrations in an elderly population. American Journal of Clinical Nutrition 62, 1228–1233.CrossRefGoogle Scholar
Ji, LL (1999) Antioxidants and oxidative stress in exercise. Proceedings of the Society for Experimental Biology and Medicine 222, 283–292.CrossRefGoogle ScholarPubMed
Kanter, MM, Nolte, LA & Holloszy, JO (1993) Effects of an antioxidant vitamin mixture on lipid peroxidation at rest and postexercise. Journal of Applied Physiology 74, 965–969.CrossRefGoogle ScholarPubMed
Levine, M, Conry-Cantilena, C, Wang, Y, Welch, RW, Washko, PW, Dhariwal, KR, Park, JB, Lazarev, A, Graumlich, JF, King, J & Cantilena, LR (1996) Vitamin C pharmacokinetics in healthy volunteers: Evidence for a recommended dietary allowance. Proceedings of the National Academy of Sciences, USA 93, 3704–3709.Google Scholar
McCabe, DR, Maher, TJ & Acworth, IA (1997) Improved method for the estimation of hydroxyl free radical levels in vivo based on liquid chromatography with electrochemical detection. Journal of Chromatography B 691, 23–32.Google Scholar
Maxwell, SR, Jakeman, P, Thomason, H, Leguen, C & Thorpe, GH (1993) Changes in plasma antioxidant status during eccentric exercise and the effect of vitamin supplementation. Free Radical Research Communications 19, 191–202.CrossRefGoogle ScholarPubMed
Mecocci, P, Fano, G, Fulle, S, MacGarvey, U, Shinobu, L, Polidori, MC, Cherubini, A, Vecchiet, J, Senin, U & Beal, MF (1999) Age-dependent increases in oxidative damage to DNA, lipids, and proteins in human skeletal muscle. Free Radicals in Biology and Medicine 26, 303–308.CrossRefGoogle ScholarPubMed
Meijer, EP, Coolen, SAJ, Bast, A & Westerterp, KR (2001) Exercise training in the elderly as measured by antipyrine hydroxylation products. Free Radical Research.(In the Press).CrossRefGoogle ScholarPubMed
Meijer, EP, Westerterp, KR & Verstappen, FTJ (1999) Effect of exercise training on total daily physical activity in elderly humans. European Journal of Applied Physiology 80, 16–21.CrossRefGoogle ScholarPubMed
Meydani, M, Evans, WJ, Handelman, G, Biddle, L, Fielding, RA, Meydani, SN, Burrill, J, Fiatarone, MA, Blumberg, JB & Cannon, JG (1993) Protective effect of vitamin E on exercise-induced oxidative damage in young and older adults. American Journal of Physiology 264, R992–R998.Google ScholarPubMed
Meydani, SN, Meydani, M, Rall, LC, Morrow, F & Blumberg, JB (1994) Assessment of the safety of high-dose, short-term supplementation with vitamin E in healthy older adults. American Journal of Clinical Nutrition 60, 704–709.CrossRefGoogle ScholarPubMed
Packer, JE, Slater, TF & Wilson, RL (1979) Direct observation of a free radical interaction between vitamin E and vitamin C. Nature 278, 737–738.CrossRefGoogle ScholarPubMed
Pallast, EG, Schouten, EG, De Waart, FG, Fonk, HF, Doekes, G, Von Blomberg, BM & Kok, FJ (1999) Effect of 50- and 100-mg vitamin E supplements on cellular immune function in noninstitutionalized elderly persons. American Journal of Clinical Nutrition 69, 1273–1281.Google Scholar
Polidori, MC, Mecocci, P, Cherubini, A & Senin, U (2000) Physical activity and oxidative stress during aging. International Journal of Sports Medicine 21, 154–157.CrossRefGoogle ScholarPubMed
Powers, SK, Criswell, D, Lawler, J, Ji, LL, Martin, D, Herb, RA & Dudley, G (1994) Influence of exercise and fiber type on antioxidant enzyme activity in rat skeletal muscle. American Journal of Physiology 266, R375–R380.Google ScholarPubMed
Sastre, J, Asensi, M, Gasco, E, Pallardo, FV, Ferrero, JA, Furukawa, T & Vina, J (1992) Exhaustive physical exercise causes oxidation of glutathione status in blood: prevention by antioxidant administration. American Journal of Physiology 263, R992–R995.Google Scholar
Shigenaga, MK, Hagen, TM & Ames, BN (1994) Oxidative damage and mitochondrial decay in aging. Proceedings of the National Academy of Sciences, USA 91, 10771–10778.Google Scholar
Sies, H & Stahl, W (1995) Vitamins E and C, β-carotene, and other carotenoids as antioxidants. American Journal of Clinical Nutrition 62, 1315S–1321S.CrossRefGoogle Scholar
Siri, WE (1956) The gross composition of the body. Advances in Biological Medicine and Physiology 4, 239–280.CrossRefGoogle ScholarPubMed
Wijnen, MH, Coolen, SAJ, Vader, HL, Reijenga, JC, Huf, FA & Roumen, RM (2001) Antioxidants reduce oxidative stress in claudicants. Journal of Surgical Research 96, 183–187.Google Scholar
Witt, EH, Reznick, AZ, Viguie, CA, Starke Reed, P & Packer, L (1992) Exercise, oxidative damage and effects of antioxidant manipulation. Journal of Nutrition 122, 766–773.CrossRefGoogle ScholarPubMed