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Antioxidant supplementation and exercise-induced oxidative stress in the 60-year-old as measured by antipyrine hydroxylates

Published online by Cambridge University Press:  09 March 2007

Erwin P. Meijor ,
Annelies H. C. Goris ,
Joan Senden ,
Joost L. J. van Dongen ,
Aalt Bast  and
Klass R. Westerterp
Erwin P. Meijor*
Affiliation:
Department of Human Biology, Maastricht University,, Maastricht,, The Netherlands
Annelies H. C. Goris
Affiliation:
Department of Human Biology, Maastricht University,, Maastricht,, The Netherlands
Joan Senden
Affiliation:
Department of Human Biology, Maastricht University,, Maastricht,, The Netherlands
Joost L. J. van Dongen
Affiliation:
Laboratory for Macromolecular and Organic Chemistry, Eindhoven University of Technology, Eindhoven, The Netherlands
Aalt Bast
Affiliation:
Department of Pharmacology and Toxicology, Maastricht University, Maastricht, The Netherland
Klass R. Westerterp
Affiliation:
Department of Human Biology, Maastricht University,, Maastricht,, The Netherlands
*
*Corresponding author: Dr Erwin P. Meijer, fax +31 43 3670976, email [email protected]
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Abstract

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The effects of 12 weeks of antioxidant supplementation on exercise-induced oxidative stress were investigated in older adults (60 (SE 1) YEARS; BMI 26 (se 1) kg/m2). Subjects were randomly divided in two groups: supplementation (n 11) with 100 mg dl-α-tocopheryl acetate, 200 mg ascorbic acid, and 2 mg β-carotene, and placebo (n 9). Before and after the 12 week supplementation period, subjects cycled for 45 min at submaximal intensity (50 % maximal workload capacity). Antipyrine was used as marker for oxidative stress. Antipyrine reacts quickly with hydroxyl radicals to form para- and ortho-hydroxyantipyrine. The latter metabolite is not formed in man through the mono-oxygenase pathway of cytochrome P450. Daily supplementation significantly increased plasma concentrations of α-tocopherol and β-carotene in the supplemented group (Δ 14·4 (se 3·2) and 0·4 (se 0·1) μmol/l; P<0·001 and P<0·01). No significant differences, within and between groups, were observed in the exercise-induced increase in the ratios para- and ortho-hydroxyantipyrine to antipyrine. In addition, supplementation did not affect the exercise-induced increase in thiobarbituric acid reactive substances in plasma. In conclusion, in 60-year-old subjects antioxidant supplementation had no effect on the exercise-induced increase in oxidative stress as measured by free radical products of antipyrine.

Keywords

AgeingAntipyrineFree radicalsAntioxidant vitamins
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 5 , November 2001 , pp. 569 - 575
Copyright
Copyright © The Nutrition Society 2001

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