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An in vitro procedure for studying enzymic dephosphorylation of phytate in maize-soyabean feeds for turkey poults

Published online by Cambridge University Press:  09 March 2007

Krzysztof Zyla ,
David R. Ledoux ,
Angel Garcia  and
Trygvel Veum
Krzysztof Zyla
Affiliation:
Department of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
David R. Ledoux
Affiliation:
Department of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
Angel Garcia
Affiliation:
Department of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
Trygvel Veum
Affiliation:
Department of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
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Abstract

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An in vitro method was developed to predict inorganic P release from maize-soyabean poultry feeds containing supplemental phytase (EC31·3·8), and to quantify the effect of acid phosphatase (EC 3·1·3·2), fungal protease (EC 3·4·23·6) and Aspergillus niger cellulase (EC 3·2·1·4) on phytate dephosphorylation. Pepsin (EC3·4·23·1) and pancreatin digestion periods were preceded by a 30 min pre-incubation at pH 5·25 to simulate digestion in the crop of poultry. Pancreatin digestion was carried out in dialysis tubing, with a ratio of about 1:25 (v/v) between the digesta and dialysing medium, to simulate gradient absorption from the duodenum. The feed:water ratio was kept within physiological limits and a constant proportion of feed weight to digestive enzymes was maintained. There was a linear response to increasing dosages of phytase up to 1000 phytase units (FTU)/kg feed, and to increasing phosphate concentration in feeds. In vivo validation was performed with growing turkeys (1–3 weeks) fed on diets containing 12 g Ca/kg and 0, 500 or 1000 FTU phytase/kg in a factorial arrangement with 0, 1, 2 or 3 g supplemental phosphate/kg (from KH2PO4). After a simple transformation (variable/in vitro P = f (in vitro P)), amounts of P hydrolysed from feed samples by in vitro digestions correlated with 3-week body-weight gain (R 0·986, P < 0·0001), toe ash (R 0·952, P < 0·0001), feed intake (R 0·994, P < 0·0001) and feed efficiency (R 0·992, P < 0·0001). The dephosphorylating ability of phytase in vitro was significantly enhanced (P < 0·05) by the addition of acid phosphatase. Fungal acid protease and Aspergillus niger cellulase also enhanced the dephosphorylation process in vitro.

Keywords

In vitro methodFeed dephosphorylationPhytaseTurkey
Type
In vitro measurement of dephosphorylation
Information
British Journal of Nutrition , Volume 74 , Issue 1 , July 1995 , pp. 3 - 17
Copyright
Copyright © The Nutrition Society 1995

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