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Acetate clearance rate as a potential index of the availability of glucogenic precursors in ruminants fed on roughage-based diets

Published online by Cambridge University Press:  09 March 2007

P. B. Cronjé ,
J. V. Nolan  and
R. A. Leng
P. B. Cronjé
Affiliation:
Department of Biochemistry, Microbiology & Nutrition, University of New England, Armidale, NSW 2351, Australia
J. V. Nolan
Affiliation:
Department of Biochemistry, Microbiology & Nutrition, University of New England, Armidale, NSW 2351, Australia
R. A. Leng
Affiliation:
Department of Biochemistry, Microbiology & Nutrition, University of New England, Armidale, NSW 2351, Australia
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Abstract

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Three experiments were conducted to investigate interactions between acetate and glucose metabolism in sheep fed on roughage-based diets, and to establish whether the clearance rate of an intravenous acetate load would provide a valid index of the dietary acetate:glucogenic precursors ratio. In Expt 1 lambs were fed on a basal diet of wheat straw and supplemented with propionate and protein. Both supplements increased glucose irreversible loss rate (ILR) although not to the same degree. Acetate clearance rates were increased by protein and propionate supplementation and were positively related to glucose ILR irrespective of precursor. In Expt 2 the effects of an increased dietary load of acetate given with or without propionate were investigated. Glucose ILR did not respond to acetate supplementation, but was increased when propionate was fed in addition to acetate. This was reflected in an unchanged ability to clear an intravenous acetate load from the blood when acetate alone was added, but an increased acetate clearance rate when propionate was fed in addition to acetate. In Expt 3 the effects of supplementation with various propionate: acetate ratios were investigated. Acetate clearance was consistently increased by an increased propionate: acetate ratio. These results show that the metabolism of excess acetate is responsive to the dietary supply of glucose precursors, and provide support for the concept that additional glucose precursors are necessary for the efficient utilization of acetate when roughage diets low in protein are fed

Keywords

Acetate clearance rateGluconeogenesisRuminant
Type
Metabolic and Physiological Effects of Fermentation
Information
British Journal of Nutrition , Volume 66 , Issue 2 , September 1991 , pp. 301 - 312
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Annison, E. F., Brown, R. E., Leng, R. A., Lindsay, D. B. & West, C. E. (1967). Rates of entry and oxidation of acetate, glucose, D(–)-β-hydroxybutyrate, palmitate, oleate and stearate, and rates of production and oxidation of propionate and butyrate in fed and starved sheep. Biochemical Journal 104, 135–147.CrossRefGoogle ScholarPubMed
Armstrong, D. G. & Blaxter, K. L. (1957). The heat increment of steam-volatile fatty acids in fasting sheep. British Journal of Nutrition 11, 247–272.CrossRefGoogle ScholarPubMed
Baldwin, R. L., Yang, Y. T. & Grichting, G. (1976). Theoretical model of ruminant adipose tissue metabolism in relation to the whole animal. Federation Proceedings 35, 2314–2318.Google Scholar
Black, J. L., Gill, M. & Thornley, J. H. M. (1984). Efficiency of acetate utilization by ruminants. In Ruminant Physiology – Concepts and Consequences, p. 288 [Baker, S. K.Gawthorn, J. M.Mackintosh, J. B. and Purser, D. B., editors]. Perth: University of Western Australia.Google Scholar
Blaxter, K. L. (1979). Discussion paper: use of energy for maintenance and growth. In Energy Metabolism, pp. 183–187 [Mount, L. E., editor]. London: Butterworths.Google Scholar
Cronjé, P. B. (1983). Protein degradation in the rumen – The effect of basal diet and a comparison of techniques. MSc Thesis, University of Stellenbosch.Google Scholar
Egan, A. R. (1965). Nutritional status and intake regulation in sheep. Australian Journal of Agricultural Research 16, 473–483.CrossRefGoogle Scholar
Egan, A. R. (1977). Nutritional status and intake regulation in sheep. VIII. Relationships between the voluntary intake of herbage by sheep and the protein/energy ratio in the digestion products. Australian Journal of Agricultural Research 28, 907–915.CrossRefGoogle Scholar
Giesecke, D. (1983). Plasma free fatty acids. In Dynamic Biochemistry of Animal Production, pp. 197–214 [Riis, P. M. editor]. Amsterdam: Elsevier.Google Scholar
Hennessy, D. W. (1984). The role of protein in improving production of cattle grazing native pastures in subtropical New South Wales. PhD Thesis, University of New England.Google Scholar
Hovell, F. D. DeB. & Greenhalgh, J. F. D. (1978). The utilization of diets containing acetate, propionate or butyrate salts by growing lambs. British Journal of Nutrition 40, 171–183.CrossRefGoogle ScholarPubMed
Hovell, F. D. DeB., Greenhalgh, J. F. D. & Wainman, F. W. (1976). The utilization of diets containing acetate salts by growing lambs as measured by comparative slaughter and respiration calorimetry, together with rumen fermentation. British Journal of Nutrition 35, 343–363.CrossRefGoogle ScholarPubMed
Jarrett, I. G. & Filsell, O. H. (1960). The effect of diet on acetate tolerance in sheep. Australian Journal of Experimental Biology and Medical Science 38, 347–353.CrossRefGoogle ScholarPubMed
Jarrett, I. G. & Filsell, O. H. (1961). An effect of glucose on acetate metabolism in sheep. Nature 190, 1114–1115.CrossRefGoogle ScholarPubMed
Jarrett, I. G., Potter, B. J. & Filsell, O. H. (1952). Lower fatty acids in the intermediary metabolism of sheep. Australian Journal of Experimental Biology and Medical Science 30, 197–206.CrossRefGoogle ScholarPubMed
Jones, G. B. (1965). Determination of the specific activity of labeled blood glucose by liquid scintillation using glucose pentaacetate. Analytical Biochemistry 12, 249–258.CrossRefGoogle ScholarPubMed
Judson, G. J. & Leng, R. A. (1972). Estimation of the total entry rate and resynthesis of glucose in sheep using glucoses uniformly labelled with 14C and variously labelled with 3H. Australian Journal of Biological Science 25, 1313–1332.CrossRefGoogle Scholar
König, B. A., Oldham, J. D. & Parker, D. S. (1984). The effect of abomasal infusions of casein on acetate, palmitate and glucose kinetics in cows during early lactation. British Journal of Nutrition 52, 319–328.CrossRefGoogle ScholarPubMed
Lawes Agricultural Trust (1980). Genstat V. Mark 4.03, Rothamsted Experimental Station, Harpenden, Herts.Google Scholar
Lee, G. J., Hennessy, D. W., Williamson, P. J., Nolan, J. V., Kempton, T. J. & Leng, R. A. (1985). Responses to protein meal supplements by lactating beef cattle given a low-quality pasture hay. Australian Journal of Agricultural Research 36, 729–741.CrossRefGoogle Scholar
Leng, R. A. & Leonard, G. J. (1965). Measurement of the rates of production of acetic, propionic and butyric acids in the rumen of sheep. British Journal of Nutrition 19, 469–484.CrossRefGoogle ScholarPubMed
MacRae, J. C. & Lobley, G. E. (1982). Some factors which influence thermal energy losses during the metabolism of ruminants. Livestock Production Science 9, 447–456.CrossRefGoogle Scholar
Madsen, A. (1983). The molecular basis of animal production: metabolism in skeletal muscle cells. In Dynamic Biochemistry of Animal Production, pp. 9–28 [Riis, P. M., editor]. Amsterdam: Elsevier.Google Scholar
Ørskov, E. R. & McDonald, I. (1979). Utilization of volatile fatty acids for maintenance and for energy retention. In Energy Metabolism, pp. 147–150 [Mount, L. E., editor]. London: Butterworths.Google Scholar
Pethick, D. W., Lindsay, D. B., Parker, P. J. & Northrop, A. J. (1981). Acetate supply and utilization by the tissues of the sheep in vivo. British Journal of Nutrition 46, 97–110.CrossRefGoogle ScholarPubMed
Ponto, K. H. & Bergen, W. G. (1974). Developmental aspects of glucose and VFA metabolism in the germfree and conventional ruminant. Journal of Animal Science 38, 893–899.CrossRefGoogle ScholarPubMed
Preston, T. R. & Leng, R. A. (1987). Matching Ruminant Production Systems with Available Resources in the Tropics and Sub-Tropics. Armidale, Australia: Penambul Books.Google Scholar
Pugh, P. S. & Scarisbrick, R. (1952). Acetate metabolism in ovine ketosis. Nature 170, 978–979.CrossRefGoogle ScholarPubMed
Reid, R. L. (1958). Studies on the carbohydrate metabolism of sheep. VII. Intravenous glucose and acetate tolerance tests. Australian Journal of Agricultural Research 9, 788–796.CrossRefGoogle Scholar
Shipley, R. A. & Clarke, R. E. (1972). Tracer Methods for in vivo Kinetics. Theory and Applications. London: Academic Press.Google Scholar
Tyrrell, H. F., Reynolds, P. J. & Moe, P. W. (1979). Effect of diet on partial efficiency of acetate use for body tissue synthesis by mature cattle. Journal of Animal Science 48, 598–606.CrossRefGoogle ScholarPubMed
Van der Walt, J. G. (1984). Metabolic interactions of lipogenic precursors in the ruminant. In Herbivore Nutrition in the Subtropics and Tropics, pp. 571–593 [F. M. C. Gilchrist and R. I. Mackie, editors]. Craighall, South Africa: The Science Press.Google Scholar
Vernon, R. G. (1981). Lipid metabolism in the adipose tissue of ruminant animals. In Lipid Metabolism in Ruminant Animals, pp. 279–362 [Christie, W. W., editor]. Oxford: Pergamon Press.CrossRefGoogle Scholar
Vernon, R. G. (1989). Endocrine control of metabolic adaptation during lactation. Proceedings of the Nutrition Society 48, 23–32.CrossRefGoogle ScholarPubMed
Weston, R. H. (1966). The effect of level of feeding on acetate tolerance in the sheep. Australian Journal of Agricultural Research 17, 933–937.CrossRefGoogle Scholar
Yang, Y. T. & Baldwin, R. L. (1973). Preparation and metabolism of isolated cells from bovine adipose tissue. Journal of Dairy Science 56, 350–365.CrossRefGoogle ScholarPubMed