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Ganoderma lucidum dry extract supplementation modulates T lymphocyte function in older women

Published online by Cambridge University Press:  27 May 2024

Patricia Nancy Iser-Bem ,
Tiago Bertola Lobato Open the ORCID record for Tiago Bertola Lobato [Opens in a new window] ,
Amanda Lins Alecrim-Zeza ,
Laiane Cristina dos Santos de Oliveira ,
Maria Elizabeth Pereira Passos ,
Richelieau Manuel ,
Vinícius Leonardo Sousa Diniz ,
Ilana Souza Correa ,
Sarah Poma de Oliveira  and
Eliane Borges da Silva
...Show all authors
Patricia Nancy Iser-Bem
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil National Commercial Learning Service (SENAC), São Paulo, Brazil
Tiago Bertola Lobato*
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Amanda Lins Alecrim-Zeza
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Laiane Cristina dos Santos de Oliveira
Affiliation:
Butantan Institute, Sao Paulo, Brazil
Maria Elizabeth Pereira Passos
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Richelieau Manuel
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Vinícius Leonardo Sousa Diniz
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Ilana Souza Correa
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Sarah Poma de Oliveira
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Eliane Borges da Silva
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Mariana Mendes de Almeida
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Beatriz Belmiro Dias
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Raquel Bragante Gritte
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Adriana Cristina Levada-Pires
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Laureane Nunes Masi
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil Multicenter Graduate Program in Physiological Sciences, Department of Physiological Sciences, Center of Biological Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil
Elaine Hatanaka
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Tania Cristina Pithon-Curi
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
Sandro Massao Hirabara
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
João Paulo Fabi
Affiliation:
Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences – Food Research Center (FoRC) – University of São Paulo, São Paulo, Brazil
Rui Curi
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil Butantan Institute, Sao Paulo, Brazil
Renata Gorjao
Affiliation:
Interdisciplinary Post-graduate Program in Health Sciences, Cruzeiro do Sul University, Sao Paulo, Brazil
*
*Corresponding author: Tiago Bertola Lobato, email [email protected]
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Abstract

Ganoderma lucidum (a mushroom used in traditional Chinese medicine) compounds may attenuate ageing-related physiological changes and restore normal immunity. However, studies on the physiological effects of Ganoderma lucidum dry extract food supplements are few. Therefore, here, we aimed to investigate the effects of Ganoderma lucidum dry extract food supplement on the lymphocyte function of older women. This was a double-blind clinical trial (n 60) with a final 39 older volunteers, divided into two groups Ganoderma lucidum (n 23) and placebo (n 16). The Ganoderma lucidum group received 2000 mg/d of Ganoderma lucidum dry extract for 8 weeks. We used flow cytometry to determine the lymphocyte profile. CD4+ lymphocyte gene expression was evaluated by real-time polymerase chain reaction. We observed that in the Ganoderma lucidum group, concanavalin A stimulation increased lymphocyte proliferation. Further, we observed an increase in expression of Forkhead box P3, transforming growth factor-beta, IL-10, IL-6, retinoic acid receptor-related orphan receptor gamma, GATA-binding protein 3 and interferon gamma genes in the Ganoderma lucidum group. Furthermore, in the Ganoderma lucidum group, ionomycin and phorbol 12-myristate 13-acetate stimulation led to decrease in Th17+ cells and increase in Th2+ cells. Thus, in older women, Ganoderma lucidum regulates T lymphocyte function leading to a predominant anti-inflammatory action but does not induce T lymphocyte proliferation through CD28 signalling pathway.

Keywords

Ling ZhiImmunomodulationCD4+ cellsOlder adultsTh17
Type
Research Article
Information
British Journal of Nutrition , Volume 132 , Issue 2 , 28 July 2024 , pp. 130 - 140
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Copyright
© The Author(s), 2024. Published by Cambridge University Press on behalf of The Nutrition Society

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