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2-Aminoethylphosphonic acid as an indicator of Tetrahymena pyriformis W growth in protein-quality evaluation assay

Published online by Cambridge University Press:  09 March 2007

Julita Maciejewicz-Ryś  and
Anna M. Antoniewicz
Julita Maciejewicz-Ryś
Affiliation:
Department of Animal Nutrition, Institute of Animal Husbandry, Skr. 161, 30-960 Kraków, Poland
Anna M. Antoniewicz
Affiliation:
Department of Animal Nutrition, Institute of Animal Husbandry, Skr. 161, 30-960 Kraków, Poland
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Abstract

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1. The concentration of 2-aminoethylphosphonic acid (AEP) in 96 h cultures of Tetrahymena pyriformis W was studied in order to apply it as an indicator in the assay of the relative nutritive value (RNV; protozoa population with test protein: protozoa population with whole-egg powder) of protein. Foodstuffs and food mixtures of different protein contents and qualities were used as test samples.

2. RNV values based on AEP determination (RNVAEP) were compared with corresponding values calculated from protozoa counts (RNVpc), as well as with biological value (bv) and net protein utilization (npu) of the same proteins assayed on rats.

3. Both for foodstuffs and food mixtures highly significant correlations were found between RNVAEP and RNVpc, RNVAEP and both bv and npu, and RNVpc, and both bv and npu.

4. AEP content in the protozoal suspension was preferred to cell count as a measure of growth response, since it took into account large differences in cell dimensions that were observed between cultures grown with different test proteins.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 40 , Issue 1 , July 1978 , pp. 83 - 90
Copyright
Copyright © The Nutrition Society 1978

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