Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-25T04:58:31.710Z Has data issue: false hasContentIssue false

Simplified polymerase chain reaction (PCR)-based sexing assists conservation of an endangered owl, the Norfolk Island Boobook Ninox novaeseelandiae undulata

Published online by Cambridge University Press:  11 May 2010

Michael Double
Affiliation:
Division of Botany and Zoology, Australian National University, ACT 0200, Australia
Penny Olsen
Affiliation:
Division of Botany and Zoology, Australian National University, ACT 0200, Australia
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In 1986 a single Norfolk Island Boobook Owl Ninox novaeseelandiae undulata remained. As part of a re-establishment programme, two male New Zealand Moreporks N. n. novaeseelandiae were introduced, one of which survived to pair with the female in the wild and breed successfully. By 1995 the population numbered 12 or 13 individuals of which seven were second generation (F2). However, there were only two breeding pairs. As the 11 hybrids could not be sexed using morphometrics we developed a molecular method based on a recently described avian polymerase chain reaction (PCR)-based sexing technique. The population was found to contain six females and five males. A scarcity of mature males was established as the main factor slowing the recovery effort.

Type
Research Article
Copyright
Copyright © Birdlife International 1997

References

Avise, J. C. (1994) Molecular markers, natural history and evolution. New York: Chapman and Hall.CrossRefGoogle Scholar
Avise, J. C. (1996) Three fundamental contributions of molecular genetics to avian ecology and evolution. Ibis 138: 1625.CrossRefGoogle Scholar
Clarke, R.J., D.G., Smith and Kelso, H. (1978) Working bibliography of owls of the world. Washington: Raptor Information Centre, National Wildlife Federation.Google Scholar
Dhondt, A. A. (1996) Molecular techniques in conservation and evolutionary biology: a quantum leap? TREE 11: 147148.Google Scholar
Garnett, S. (1992) Threatened and extinct birds of Australia. RAOU Report 82.Google Scholar
Griffiths, R. and Tiwari, B. (1993) The isolation of genetic markers for the identification of sex. Proc. Natn. Acad. Sci. USA 90: 83248326.CrossRefGoogle ScholarPubMed
Griffiths, R. and Tiwari, B. (1995) Sex of the last wild Spix's Macaw. Nature 375: 454.CrossRefGoogle ScholarPubMed
Griffiths, R., Daan, S. and Dijkstra, C. (1996) Sex identification in birds using two CHD genes. Proc. Roy. Soc. (hand.) B263: 12511256.Google Scholar
Lessells, K. and Mateman, C. (1996) Molecular sexing of birds. Nature 383: 761762.Google Scholar
Olsen, P. D. (1996) Re-establishment of an endangered subspecies: the Norfolk Island Boobook Owl Ninox novaeseelandiae undulata. Bird Conserv. Internatn. 6: 6380.CrossRefGoogle Scholar
Olsen, P. D., Mooney, N. J. and Olsen, J. (1989) Status'and conservation of the Norfolk Island Boobook Ninox novaeseelandiae undulata. Pp. 415422 in Meyberg, B.-U. and Chancellor, R.D., eds. Raptors in the modern world. London: World Working Group on Birds of Prey.Google Scholar