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Proteomic comparison of outer membrane protein patterns of sessile and planktonic Pseudomonas aeruginosa cells

Published online by Cambridge University Press:  07 March 2005

D. Seyer ,
P. Cosette ,
A. Siroy ,
E. Dé ,
C. Lenz ,
H. Vaudry ,
L. Coquet  and
T. Jouenne
D. Seyer
Affiliation:
IBBR Group, Laboratory “Polymères, Biopolymères, Membranes”, UMR 6522 CNRS, University of Rouen, France Institute for Peptide Research (IFRMP23), University of Rouen, France
P. Cosette
Affiliation:
IBBR Group, Laboratory “Polymères, Biopolymères, Membranes”, UMR 6522 CNRS, University of Rouen, France Institute for Peptide Research (IFRMP23), University of Rouen, France
A. Siroy
Affiliation:
IBBR Group, Laboratory “Polymères, Biopolymères, Membranes”, UMR 6522 CNRS, University of Rouen, France Institute for Peptide Research (IFRMP23), University of Rouen, France
E. Dé
Affiliation:
IBBR Group, Laboratory “Polymères, Biopolymères, Membranes”, UMR 6522 CNRS, University of Rouen, France Institute for Peptide Research (IFRMP23), University of Rouen, France
C. Lenz
Affiliation:
Applied Biosystems, Frankfurter Strasse 129 B, 64291 Darmstadt, Germany
H. Vaudry
Affiliation:
Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U-413, UA CNRS, University of Rouen, France Institute for Peptide Research (IFRMP23), University of Rouen, France
L. Coquet
Affiliation:
IBBR Group, Laboratory “Polymères, Biopolymères, Membranes”, UMR 6522 CNRS, University of Rouen, France Institute for Peptide Research (IFRMP23), University of Rouen, France
T. Jouenne
Affiliation:
IBBR Group, Laboratory “Polymères, Biopolymères, Membranes”, UMR 6522 CNRS, University of Rouen, France Institute for Peptide Research (IFRMP23), University of Rouen, France
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Abstract

We have compared the outer membrane protein (OMP) pattern of sessile Pseudomonas aeruginosa cells with that of its stationary-phase free-floating counterparts. Two-dimensional electrophoretic gels were compared using computing scanning densitometry. The level of 103 OMPs (i.e. about 50% of the spots that fulfilled our statistical selection threshold) was significantly and reproducibly different in biofilm organisms as compared with their planktonic counterparts. Some OMPs that specifically either accumulated or decreased were identified by mass spectrometry and the role of these proteins in the biofilm phenotype is discussed.

Type
Research Articles
Information
Biofilms , Volume 2 , Issue 1 , January 2005 , pp. 27 - 36
Copyright
© 2005 Cambridge University Press

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