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EDTA treatment diminishes the antibacterial and anti-adherence effect of calcium hydroxide on Enterococcus faecalis: an in vitro study

Published online by Cambridge University Press:  10 November 2008

S. George  and
A. Kishen
S. George
Affiliation:
Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, 5 Lower Kent Ridge Road, Singapore119074
A. Kishen*
Affiliation:
Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, 5 Lower Kent Ridge Road, Singapore119074
*
*Corresponding author: Dr A. Kishen, Department of Restorative Dentistry, Faculty of Dentistry, National University of Singapore, National University Hospital, 5 Lower Kent Ridge Road, Singapore119074, T65 6516 4624, F65 6774 5701, E[email protected]
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Abstract

This study sought to understand the cell surface characteristics, viability and biofilm-forming potential of Enterococcus faecalis cells sequentially exposed to EDTA and calcium hydroxide, as in endodontic treatment. Bacterial cells exposed to EDTA and calcium hydroxide were assayed for cell viability, membrane integrity, cell surface hydrophobicity and surface charge, while alteration in the surface topography of E. faecalis cells was examined using atomic force microscopy (AFM). The bacterial adherence potential to type I collagen was also examined to assess the biofilm-forming capacity of E. faecalis cells exposed to EDTA and calcium hydroxide. It was found that calcium hydroxide treatment reduced the viability of E. faecalis. However, prior exposure to EDTA significantly reduced the antibacterial effect of calcium hydroxide (P < 0.05). Calcium hydroxide treatment resulted in impaired cell wall morphology, observed as increased surface roughness and pore formation under AFM. However, these topographical changes induced by calcium hydroxide were significantly reduced in EDTA pretreated cells (P < 0.05). Calcium hydroxide treatment caused reduction in hydrophobicity and adherence of E. faecalis to type I collagen. These effects due to calcium hydroxide were also significantly altered in EDTA-pretreated cells (P < 0.001). The findings from this study showed that the antibacterial and anti-adherence effect of calcium hydroxide was diminished by prior exposure of E. faecalis cells to EDTA.

Type
Research Article
Information
Biofilms , First View , pp. 1 - 10
Copyright
Copyright © Cambridge University Press 2008

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